Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
June-2014 Volume 7 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
June-2014 Volume 7 Issue 6

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Identification of microRNA profiles in salivary adenoid cystic carcinoma cells during metastatic progression

  • Authors:
    • Wei Chen
    • Xiaoge Zhao
    • Zhen Dong
    • Gang Cao
    • Senlin Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Stomatology, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, P.R. China, Key Laboratory of Environment and Genes Related to Diseases, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi 710061, P.R. China, Department of Stomatology, Nanjing Jinling Hospital, Nanjing University School of Medicine, Nanjing, Jiangsu 210002, P.R. China
  • Pages: 2029-2034
    |
    Published online on: March 14, 2014
       https://doi.org/10.3892/ol.2014.1975
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Salivary adenoid cystic carcinoma (SACC) is a common type of salivary gland cancer. The poor long‑term prognosis of patients with SACC is primarily due to local recurrence, distant metastasis and perineural invasion. MicroRNAs (miRNAs) have been identified as important post‑transcriptional regulators, which are involved in various biological processes. The aim of the present study was to identify the miRNA expression profiles that are involved in the metastatic progression of SACC. Therefore, microarray technology was employed to identify miRNA expression profiles in an SACC cell line, ACC‑2, and a highly metastatic SACC cell line, ACC‑M, which was screened from ACC‑2 by a combination of in vivo selection and cloning in vitro. Differences in miRNA expression were assessed by quantitative polymerase chain reaction (qPCR) assay. In addition, the potential target genes that are regulated by selected miRNAs were analyzed by various target prediction tools. The microarray data revealed that the levels of 38 miRNAs significantly differed between the ACC‑M cells and the control ACC‑2 cells. Six miRNAs (miR‑4487, ‑4430, ‑486‑3p, ‑5191, ‑3131 and ‑211‑3p) were selected to validate the microarray data via qPCR. The expression of two miRNAs (miR‑4487 and ‑4430) was significantly upregulated in the ACC‑M cells, while the expression of two other miRNAs (miR‑5191 and ‑3131) was significantly downregulated in the ACC‑M cells. The potential target genes that were identified to be controlled by the six selected miRNAs were divided into four groups according to function, as follows: Apoptosis and proliferation (46 genes), cell cycle (30 genes), DNA damage and repair (24 genes) and signaling pathway (30 genes). The identification of microRNA expression profiles in highly metastatic SACC cells may provide an improved understanding of the mechanisms involved in metastatic progression, which would aid in the development of novel strategies for the treatment of SACC.
View Figures

Figure 1

Figure 2

View References

1 

Renehan A, Gleave EN, Hancock BD, Smith P and McGurk M: Long-term follow-up of over 1000 patients with salivary gland tumours treated in a single centre. Br J Surg. 83:1750–1754. 1996. View Article : Google Scholar : PubMed/NCBI

2 

Anderson JN Jr, Beenken SW, Crowe R, Soong SJ, Peters G, Maddox WA and Urist MM: Prognostic factors in minor salivary gland cancer. Head Neck. 17:480–486. 1995. View Article : Google Scholar : PubMed/NCBI

3 

van der Wal JE, Becking AG, Snow GB and van der Waal I: Distant metastases of adenoid cystic carcinoma of the salivary glands and the value of diagnostic examinations during follow-up. Head Neck. 24:779–783. 2002.PubMed/NCBI

4 

Ramer N, Wu H, Sabo E, Ramer Y, Emanuel P, Orta L and Burstein DE: Prognostic value of quantitative p63 immunostaining in adenoid cystic carcinoma of salivary gland assessed by computerized image analysis. Cancer. 116:77–83. 2010.PubMed/NCBI

5 

Chen W, Zhang HL, Shao XJ, Jiang YG, Zhao XG, Gao X, Li JH, Yang J, Zhang YF, Liu BL and Sun MY: Gene expression profile of salivary adenoid cystic carcinoma associated with perineural invasion. Tohoku J Exp Med. 212:319–334. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Nakashima D, Uzawa K, Kasamatsu A, Koike H, Endo Y, Saito K, Hashitani S, Numata T, Urade M and Tanzawa H: Protein expression profiling identifies maspin and stathmin as potential biomarkers of adenoid cystic carcinoma of the salivary glands. Int J Cancer. 118:704–713. 2006. View Article : Google Scholar

7 

Chen W, Zhang H, Wang J, Cao G, Dong Z, Su H, Zhou X and Zhang S: Lentiviral-mediated gene silencing of Notch-4 inhibits in vitro proliferation and perineural invasion of ACC-M cells. Oncol Rep. 29:1794–1804. 2013.

8 

Ding LC, She L, Zheng DL, Huang QL, Wang JF, Zheng FF and Lu YG: Notch-4 contributes to the metastasis of salivary adenoid cystic carcinoma. Oncol Rep. 24:363–368. 2010.PubMed/NCBI

9 

Yang X, Zhang P, Ma Q, Kong L, Li Y, Liu B and Lei D: EMMPRIN silencing inhibits proliferation and perineural invasion of human salivary adenoid cystic carcinoma cells in vitro and in vivo. Cancer Biol Ther. 13:85–91. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Luukkaa H, Klemi P, Hirsimäki P, Vahlberg T, Kivisaari A, Kähäri VM and Grénman R: Matrix metalloproteinase (MMP)-1, -9 and -13 as prognostic factors in salivary gland cancer. Acta Otolaryngol. 128:482–490. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Hao L, Xiao-lin N, Qi C, Yi-ping Y, Jia-quan L and Yan-ning L: Nerve growth factor and vascular endothelial growth factor: retrospective analysis of 63 patients with salivary adenoid cystic carcinoma. Int J Oral Sci. 2:35–44. 2010. View Article : Google Scholar : PubMed/NCBI

12 

Costa AF, Tasso MG, Mariano FV, Soares AB, Chone CT, Crespo AN, Fresno MF, Llorente JL, Suárez C, de Araújo VC, Hermsen M and Altemani A: Levels and patterns of expression of hypoxia-inducible factor-1α, vascular endothelial growth factor, glucose transporter-1 and CD105 in adenoid cystic carcinomas with high-grade transformation. Histopathology. 60:816–825. 2012.

13 

Chekanova JA and Belostotsky DA: MicroRNAs and messenger RNA turnover. Methods Mol Biol. 342:73–85. 2006.PubMed/NCBI

14 

Lages E, Ipas H, Guttin A, Nesr H, Berger F and Issartel JP: MicroRNAs: molecular features and role in cancer. Front Biosci (Landmark Ed). 17:2508–2540. 2012. View Article : Google Scholar

15 

Li C, Hashimi SM, Good DA, Cao S, Duan W, Plummer PN, Mellick AS and Wei MQ: Apoptosis and microRNA aberrations in cancer. Clin Exp Pharmacol Physiol. 39:739–46. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Nishizawa T and Suzuki H: The role of microRNA in gastric malignancy. Int J Mol Sci. 14:9487–9496. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Wu WK, Lee CW, Cho CH, Fan D, Wu K, Yu J and Sung JJ: MicroRNA dysregulation in gastric cancer: a new player enters the game. Oncogene. 29:5761–5771. 2010. View Article : Google Scholar : PubMed/NCBI

18 

Guan XF, Qiu WL, He RG and Zhou XJ: Selection of adenoid cystic carcinoma cell clone highly metastatic to the lung: an experimental study. Int J Oral Maxillofac Surg. 26:116–119. 1997. View Article : Google Scholar : PubMed/NCBI

19 

Vela J, Vitorica J and Ruano D: Rapid PCR-mediated synthesis of competitor molecules for accurate quantification of beta(2) GABA(A) receptor subunit mRNA. Brain Res Brain Res Protoc. 8:184–190. 2001. View Article : Google Scholar : PubMed/NCBI

20 

Brazma A, Hingamp P, Quackenbush J, Sherlock G, Spellman P, Stoeckert C, Aach J, Ansorge W, Ball CA, Causton HC, et al: Minimum information about a microarray experiment (MIAME)-toward standards for microarray data. Nat Genet. 29:365–371. 2001. View Article : Google Scholar : PubMed/NCBI

21 

Liu SG, Qin XG, Zhao BS, Qi B, Yao WJ, Wang TY, Li HC and Wu XN: Differential expression of miRNAs in esophageal cancer tissue. Oncol Lett. 5:1639–1642. 2013.PubMed/NCBI

22 

Margue C, Philippidou D, Reinsbach SE, Schmitt M, Behrmann I and Kreis S: New target genes of MITF-induced microRNA-211 contribute to melanoma cell invasion. PLoS One. 8:e734732013. View Article : Google Scholar : PubMed/NCBI

23 

Nikitina EG, Urazova LN and Stegny VN: MicroRNAs and human cancer. Exp Oncol. 34:2–8. 2012.

24 

Matsuba HM, Spector GJ, Thawley SE, Simpson JR, Mauney M and Pikul FJ: Adenoid cystic salivary gland carcinoma. A histopathologic review of treatment failure patterns. Cancer. 57:519–524. 1986. View Article : Google Scholar : PubMed/NCBI

25 

Kim KH, Sung MW, Chung PS, Rhee CS, Park CI and Kim WH: Adenoid cystic carcinoma of the head and neck. Arch Otolaryngol Head Neck Surg. 120:721–726. 1994. View Article : Google Scholar : PubMed/NCBI

26 

Wiseman SM, Popat SR, Rigual NR, Hicks WL Jr, Orner JB, Wein RO, McGary CT and Loree TR: Adenoid cystic carcinoma of the paranasal sinuses or nasal cavity: a 40-year review of 35 cases. Ear Nose Throat J. 81:510–517. 2002.PubMed/NCBI

27 

Fordice J, Kershaw C, El-Naggar A and Goepfert H: Adenoid cystic carcinoma of the head and neck: predictors of morbidity and mortality. Arch Otolaryngol Head Neck Surg. 125:149–152. 1999. View Article : Google Scholar : PubMed/NCBI

28 

Sequeiros-Santiago G, García-Carracedo D, Fresno MF, Suarez C, Rodrigo JP and Gonzalez MV: Oncogene amplification pattern in adenoid cystic carcinoma of the salivary glands. Oncol Rep. 21:1215–1222. 2009.PubMed/NCBI

29 

He Q, Zhou X, Li S, Jin Y, Chen Z, Chen D, Cai Y, Liu Z, Zhao T and Wang A: MicroRNA-181a suppresses salivary adenoid cystic carcinoma metastasis by targeting MAPK-Snai2 pathway. Biochim Biophys Acta. 1830:5258–5266. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Mitani Y, Roberts DB, Fatani H, Weber RS, Kies MS, Lippman SM and El-Naggar AK: MicroRNA profiling of salivary adenoid cystic carcinoma: association of miR-17–92 upregulation with poor outcome. PLoS One. 8:e667782013.PubMed/NCBI

31 

Liu L, Hu Y, Fu J, Yang X and Zhang Z: MicroRNA155 in the growth and invasion of salivary adenoid cystic carcinoma. J Oral Pathol Med. 42:140–147. 2013. View Article : Google Scholar : PubMed/NCBI

32 

Pinós T, Barbosa-Desongles A, Hurtado A, Santamaria-Martínez A, de Torres I, Reventós J and Munell F: Human SHBG mRNA translation is modulated by alternative 5′-non-coding exons 1A and 1B. PLoS One. 5:e138442010.PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Chen W, Zhao X, Dong Z, Cao G and Zhang S: Identification of microRNA profiles in salivary adenoid cystic carcinoma cells during metastatic progression. Oncol Lett 7: 2029-2034, 2014.
APA
Chen, W., Zhao, X., Dong, Z., Cao, G., & Zhang, S. (2014). Identification of microRNA profiles in salivary adenoid cystic carcinoma cells during metastatic progression. Oncology Letters, 7, 2029-2034. https://doi.org/10.3892/ol.2014.1975
MLA
Chen, W., Zhao, X., Dong, Z., Cao, G., Zhang, S."Identification of microRNA profiles in salivary adenoid cystic carcinoma cells during metastatic progression". Oncology Letters 7.6 (2014): 2029-2034.
Chicago
Chen, W., Zhao, X., Dong, Z., Cao, G., Zhang, S."Identification of microRNA profiles in salivary adenoid cystic carcinoma cells during metastatic progression". Oncology Letters 7, no. 6 (2014): 2029-2034. https://doi.org/10.3892/ol.2014.1975
Copy and paste a formatted citation
x
Spandidos Publications style
Chen W, Zhao X, Dong Z, Cao G and Zhang S: Identification of microRNA profiles in salivary adenoid cystic carcinoma cells during metastatic progression. Oncol Lett 7: 2029-2034, 2014.
APA
Chen, W., Zhao, X., Dong, Z., Cao, G., & Zhang, S. (2014). Identification of microRNA profiles in salivary adenoid cystic carcinoma cells during metastatic progression. Oncology Letters, 7, 2029-2034. https://doi.org/10.3892/ol.2014.1975
MLA
Chen, W., Zhao, X., Dong, Z., Cao, G., Zhang, S."Identification of microRNA profiles in salivary adenoid cystic carcinoma cells during metastatic progression". Oncology Letters 7.6 (2014): 2029-2034.
Chicago
Chen, W., Zhao, X., Dong, Z., Cao, G., Zhang, S."Identification of microRNA profiles in salivary adenoid cystic carcinoma cells during metastatic progression". Oncology Letters 7, no. 6 (2014): 2029-2034. https://doi.org/10.3892/ol.2014.1975
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team