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August-2014 Volume 8 Issue 2

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

MACC1 induces metastasis in ovarian carcinoma by upregulating hepatocyte growth factor receptor c‑MET

  • Authors:
    • Xiu‑Jie Sheng
    • Zhen Li
    • Man Sun
    • Zhi‑Hui Wang
    • Dong‑Mei Zhou
    • Jian‑Qi Li
    • Qin Zhao
    • Xiao‑Fang Sun
    • Qi‑Cai Liu
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong 510150, P.R. China, Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, Guangzhou, Guangdong 510150, P.R. China, Experimental Medical Research Center of Guangzhou Medical University, Guangzhou, Guangdong 510182, P.R. China
  • Pages: 891-897
    |
    Published online on: May 27, 2014
       https://doi.org/10.3892/ol.2014.2184
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Abstract

Metastasis‑associated in colon cancer 1 (MACC1) is a newly identified gene that has been shown to promote tumor cell invasion and metastasis. The present study investigated the effect of MACC1 downregulation on the biological characteristics of the ovarian cancer OVCAR3 cell line. In this study, MACC1 expression was blocked using the RNA interference technique. The downregulation of MACC1 mRNA and protein expression was confirmed using quantitative polymerase chain reaction and western blot analysis. The proliferative activity and adhesion rate of the cells were detected using cell counting kit‑8 and a cell adhesion assay, while cell invasion was determined using a Matrigel invasion assay and migration capacity was observed using migration and wound‑healing assays. A tube formation assay was also used to examine the angiogenic capacity of cells, and a luciferase assay was performed to assess whether MACC1 binds to the c‑MET gene. The MACC1 mRNA and protein expression levels were significantly downregulated using sequence‑specific small interfering RNA (siRNA). The inhibition of MACC1 expression markedly decreased the invasive, metastatic and angiogenic capacities of the cells, but only slightly inhibited growth and adhesion. In addition, a putative MACC1‑binding site was identified in the 3'‑untranslated region of c‑MET. MACC1‑siRNA was also found to significantly reduce the expression of the c‑MET protein and a luciferase reporter assay confirmed that c‑MET was the target gene of MACC1. These results demonstrated that the attenuation of MACC1 suppresses cell invasion and migration and that MACC1 may regulate cell metastasis through targeting the expression of c‑MET. Inhibition of the function of MACC1 may represent a new strategy for treating ovarian cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Sheng XJ, Li Z, Sun M, Wang ZH, Zhou DM, Li JQ, Zhao Q, Sun XF and Liu QC: MACC1 induces metastasis in ovarian carcinoma by upregulating hepatocyte growth factor receptor c‑MET. Oncol Lett 8: 891-897, 2014.
APA
Sheng, X., Li, Z., Sun, M., Wang, Z., Zhou, D., Li, J. ... Liu, Q. (2014). MACC1 induces metastasis in ovarian carcinoma by upregulating hepatocyte growth factor receptor c‑MET. Oncology Letters, 8, 891-897. https://doi.org/10.3892/ol.2014.2184
MLA
Sheng, X., Li, Z., Sun, M., Wang, Z., Zhou, D., Li, J., Zhao, Q., Sun, X., Liu, Q."MACC1 induces metastasis in ovarian carcinoma by upregulating hepatocyte growth factor receptor c‑MET". Oncology Letters 8.2 (2014): 891-897.
Chicago
Sheng, X., Li, Z., Sun, M., Wang, Z., Zhou, D., Li, J., Zhao, Q., Sun, X., Liu, Q."MACC1 induces metastasis in ovarian carcinoma by upregulating hepatocyte growth factor receptor c‑MET". Oncology Letters 8, no. 2 (2014): 891-897. https://doi.org/10.3892/ol.2014.2184
Copy and paste a formatted citation
x
Spandidos Publications style
Sheng XJ, Li Z, Sun M, Wang ZH, Zhou DM, Li JQ, Zhao Q, Sun XF and Liu QC: MACC1 induces metastasis in ovarian carcinoma by upregulating hepatocyte growth factor receptor c‑MET. Oncol Lett 8: 891-897, 2014.
APA
Sheng, X., Li, Z., Sun, M., Wang, Z., Zhou, D., Li, J. ... Liu, Q. (2014). MACC1 induces metastasis in ovarian carcinoma by upregulating hepatocyte growth factor receptor c‑MET. Oncology Letters, 8, 891-897. https://doi.org/10.3892/ol.2014.2184
MLA
Sheng, X., Li, Z., Sun, M., Wang, Z., Zhou, D., Li, J., Zhao, Q., Sun, X., Liu, Q."MACC1 induces metastasis in ovarian carcinoma by upregulating hepatocyte growth factor receptor c‑MET". Oncology Letters 8.2 (2014): 891-897.
Chicago
Sheng, X., Li, Z., Sun, M., Wang, Z., Zhou, D., Li, J., Zhao, Q., Sun, X., Liu, Q."MACC1 induces metastasis in ovarian carcinoma by upregulating hepatocyte growth factor receptor c‑MET". Oncology Letters 8, no. 2 (2014): 891-897. https://doi.org/10.3892/ol.2014.2184
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