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Expression and significance of MMP2 and HIF‑1α in hepatocellular carcinoma

  • Authors:
    • Bin Wang
    • You‑Ming Ding
    • Ping Fan
    • Bing Wang
    • Jun‑Hui Xu
    • Wei‑Xing Wang
  • View Affiliations / Copyright

    Affiliations: Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 539-546
    |
    Published online on: May 28, 2014
       https://doi.org/10.3892/ol.2014.2189
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Abstract

Hepatocellular carcinoma (HCC) is a serious threat to human health. HCC is a malignant tumor and its invasion and metastasis are the result of multigene interactions. Matrix metalloproteinase‑2 (MMP-2) is capable of degrading the majority of components of the extracellular matrix and is regarded to closely correlate with tumor invasion and metastasis. Furthermore, the hypoxia‑inducible factor 1α (HIF‑1α) is an important transcription factor, which is closely associated with the process of tumor growth. The aim of the present study was to investigate the expression of MMP2 and HIF‑1α) in HCC, and the relationship between MMP2/HIF‑1α protein expression and the clinical/pathological characteristics of HCC. The mRNA levels of MMP2 and HIF‑1α were detected in 32 cases of HCC and the corresponding normal adjacent tissues with fluorescence‑based quantitative polymerase chain reaction (qPCR). The protein expression of MMP2 and HIF‑1α was assessed in 45 HCC cases and 33 cases of corresponding normal adjacent tissue, using immunohistochemical methods. The association between MMP2/HIF‑1α and pathological features of HCC, and the correlation between MMP2 and HIF‑1α were analyzed. The Kaplan‑Meier method was used to assess the impact of MMP2 and HIF‑1α expression on survival. The fluorescence‑based qPCR demonstrated that MMP2 and HIF‑1α mRNA expression levels in the HCC tissues were 0.84±0.17 and 0.87±0.11, respectively, which were significantly higher than those in the adjacent normal tissues (0.70±0.13 and 0.68±0.13, respectively; P<0.05). Immunohistochemical analysis revealed that MMP2 and HIF‑1α protein expression in the HCC tissues was 63.1 and 70.8%, respectively, which was also higher than that in the adjacent normal tissues (34.2 and 36.8%, respectively). There was no significant correlation between the expression of MMP2 or HIF‑1α protein and the age or gender of patients with HCC (P>0.05). However, there was significant correlation between MMP2 or HIF‑1α protein expression and tumor size, metastasis, presence of a capsule and clinical TNM staging of HCC. Their expression also had a significant effect on patient survival time. In conclusion, MMP2 and HIF‑1α are overexpressed in HCC, and the MMP2 signaling pathway may promote the development of HCC together with HIF‑1α.
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Copy and paste a formatted citation
Spandidos Publications style
Wang B, Ding YM, Fan P, Wang B, Xu JH and Wang WX: Expression and significance of MMP2 and HIF‑1α in hepatocellular carcinoma. Oncol Lett 8: 539-546, 2014.
APA
Wang, B., Ding, Y., Fan, P., Wang, B., Xu, J., & Wang, W. (2014). Expression and significance of MMP2 and HIF‑1α in hepatocellular carcinoma. Oncology Letters, 8, 539-546. https://doi.org/10.3892/ol.2014.2189
MLA
Wang, B., Ding, Y., Fan, P., Wang, B., Xu, J., Wang, W."Expression and significance of MMP2 and HIF‑1α in hepatocellular carcinoma". Oncology Letters 8.2 (2014): 539-546.
Chicago
Wang, B., Ding, Y., Fan, P., Wang, B., Xu, J., Wang, W."Expression and significance of MMP2 and HIF‑1α in hepatocellular carcinoma". Oncology Letters 8, no. 2 (2014): 539-546. https://doi.org/10.3892/ol.2014.2189
Copy and paste a formatted citation
x
Spandidos Publications style
Wang B, Ding YM, Fan P, Wang B, Xu JH and Wang WX: Expression and significance of MMP2 and HIF‑1α in hepatocellular carcinoma. Oncol Lett 8: 539-546, 2014.
APA
Wang, B., Ding, Y., Fan, P., Wang, B., Xu, J., & Wang, W. (2014). Expression and significance of MMP2 and HIF‑1α in hepatocellular carcinoma. Oncology Letters, 8, 539-546. https://doi.org/10.3892/ol.2014.2189
MLA
Wang, B., Ding, Y., Fan, P., Wang, B., Xu, J., Wang, W."Expression and significance of MMP2 and HIF‑1α in hepatocellular carcinoma". Oncology Letters 8.2 (2014): 539-546.
Chicago
Wang, B., Ding, Y., Fan, P., Wang, B., Xu, J., Wang, W."Expression and significance of MMP2 and HIF‑1α in hepatocellular carcinoma". Oncology Letters 8, no. 2 (2014): 539-546. https://doi.org/10.3892/ol.2014.2189
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