Suppressive effects of simvastatin on the human acute promyelocytic leukemia NB4 cell line through the regulation of the nuclear factor‑κB signaling pathway

Qiu,Guoqiang; Xie,Xiaobao; Wang,Zhilin; Zeng,Mei; Jiang,Tingxiu; Zou,Zhilan; Dai,Li; Hua,Xiaoying; Gu,Weiying

doi:10.3892/ol.2014.2204

August-2014 Volume 8 Issue 2

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August-2014 Volume 8 Issue 2

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Article

Suppressive effects of simvastatin on the human acute promyelocytic leukemia NB4 cell line through the regulation of the nuclear factor‑κB signaling pathway

Authors:
- Guoqiang Qiu
- Xiaobao Xie
- Zhilin Wang
- Mei Zeng
- Tingxiu Jiang
- Zhilan Zou
- Li Dai
- Xiaoying Hua
- Weiying Gu
View Affiliations / Copyright

Affiliations: Laboratory of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213003, P.R. China, Department of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213003, P.R. China, Laboratory of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213003, P.R. China, Department of Hematology, The First People's Hospital of Kunshan, Kunshan, Jiangsu 215300, P.R. China, Department of Hematology, Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou, Guangxi 545005, P.R. China, Department of Hematology, The First People's Hospital of Changzhou, Third Affiliated Hospital of Suzhou University, Changzhou, Jiangsu 213003, P.R. China
Pages: 693-698
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Published online on: May 30, 2014

https://doi.org/10.3892/ol.2014.2204
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Abstract

The present study examined the effects of simvastatin on the proliferation, apoptosis and gene expression levels involved in the nuclear factor‑κB (NF‑κB) signaling pathway in the human acute promyelocytic leukemia NB4 cell line by methyl thiazolyl tetrazolium assay, flow cytometry and the Human NF‑κB Signaling Pathway RT² Profiler™ PCR Array profiles. The results showed that simvastatin significantly inhibited proliferation and induced apoptosis of the NB4 cells in a time‑ and dose‑dependent manner. Changes were noted in the expression levels of 56 genes involved in the NF‑κB signaling pathways in the NB4 cells treated with 15 µm simvastatin at 48 h post‑incubation, among which, 47 genes were downregulated and 9 were upregulated. In conclusion, simvastatin potentially inhibits the proliferation and induces the apoptosis of NB4 cells through the regulation of the expression levels of genes involved in the NF‑κB signaling pathway.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Suppressive effects of simvastatin on the human acute promyelocytic leukemia NB4 cell line through the regulation of the nuclear factor‑κB signaling pathway

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