Regulatory effects of miRNA on gastric cancer cells

Yang,Ouyang; Jianhua,Huang; Lin,Sun

doi:10.3892/ol.2014.2232

Regulatory effects of miRNA on gastric cancer cells

Authors:
- Ouyang Yang
- Huang Jianhua
- Sun Lin
View Affiliations

Affiliations: Department of General Surgery, Xiangya Hospital of Central‑South University, Changsha, Hunan 410008, P.R. China
Published online on: June 5, 2014 https://doi.org/10.3892/ol.2014.2232
Pages: 651-656

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The present study aimed to screen the regulatory mechanism of microRNA (miR/miRNA) typing on gastric cancer cells in gastric cancer tissues. In total, 46 patients who underwent gastric resection in Xiangya Hospital of Central‑South University (Changsha, Hunan, China) between January and December 2012 were selected. Gastric cancer cells were obtained for RNA extraction, and miRNAs were detected by quantitative polymerase chain reaction. Compared with the expression levels in the normal gastric mucosa, the expression levels of miR‑9, miR‑433, miR‑19b and miR‑370 were downregulated; the differences was statistically significant, except for miR‑19b (P<0.05). miRNAs play a regulative role in the occurrence and development of gastric cancer, and changes in their expression provide a basis for the diagnosis of this disease.

View Figures

View References

1	Tavazoie SF, Alarcón C, Oskarsson T, et al: Endogenous human microRNAs that suppress breast cancer metastasis. Nature. 451:147–152. 2008.
2	Waldman SA and Terzic A: MicroRNA signature as diagnostic and therapeutic targets. Clin Chem. 54:943–944. 2008.
3	Ito T, Shimada Y, Kan T, et al: Pituitary tumor-transforming 1 increases cell motility and promotes lymph node metastasis in esophageal squamous cell carcinoma. Cancer Res. 68:3214–3224. 2008.
4	Inose H, Ochi H, Kimura A, et al: A microRNA regulatory mechanism of osteoblast differentiation. Proc Natl Acad Sci USA. 106:20794–20799. 2009.
5	Harquail J, Benzina S and Robichaud GA: MicroRNAs and breast cancer malignancy: an overview of miRNA-regulated cancer processes leading to metastasis. Cancer Biomark. 11:269–280. 2012.
6	Huang XA and Lin H: The miRNA regulation of stem cells. Wiley Interdiscip Rev Membr Transp Signal. 1:83–95. 2012.
7	Maccani MA and Marsit CJ: Exposure and fetal growth-associated miRNA alterations in the human placenta. Clin Epigenetics. 2:401–404. 2011.
8	Jethwa K, Wei J, McEnery K and Heimberger AB: miRNA-mediated immune regulation and immunotherapeutic potential in glioblastoma. Clin Investig (Lond). 1:1637–1650. 2011.
9	Lodewijk L, Prins AM, Kist JW, Valk GD, Kranenburg O, Rinkes IH and Vriens MR: The value of miRNA in diagnosing thyroid cancer: a systematic review. Cancer Biomark. 11:229–238. 2012.
10	Sanchez N, Lao N, Gallagher C, Clynes M and Barron N: Engineering CHO cell growth by stable manipulation of miRNA expression. BMC Proc. 5(Suppl 8): P222011.
11	Wang L, Liu W, Jiang W, Lin J, Jiang Y, Li B and Pang D: A miRNA binding site single-nucleotide polymorphism in the 3′-UTR region of the IL23R gene is associated with breast cancer. PLoS One. 7:e498232012.
12	Hirata H, Ueno K, Shahryari V, Tanaka Y, Tabatabai ZL, Hinoda Y and Dahiya R: Oncogenic miRNA-182-5p targets Smad4 and RECK in human bladder cancer. PLoS One. 7:e510562012.
13	Srivastava K and Srivastava A: Comprehensive review of genetic association studies and meta-analyses on miRNA polymorphisms and cancer risk. PLoS One. 7:e509662012.
14	Agirre X, Martínez-Climent JÁ, Odero MD and Prósper F: Epigenetic regulation of miRNA genes in acute leukemia. Leukemia. 26:395–403. 2012.
15	Yue SB, Trujillo RD, Tang Y, O’Gorman WE and Chen CZ: Loop nucleotides control primary and mature miRNA function in target recognition and repression. RNA Biol. 8:1115–1123. 2011.
16	Guo Y, Chen Y, Carreon S and Qiang M: Chronic intermittent ethanol exposure and its removal induce a different miRNA expression pattern in primary cortical neuronal cultures. Alcohol Clin Exp Res. 36:1058–1066. 2012.
17	Martin EC, Bratton MR, Zhu Y, Rhodes LV, Tilghman SL, Collins-Burow BM and Burow ME: Insulin-like growth factor-1 signaling regulates miRNA expression in MCF-7 breast cancer cell line. PLoS One. 7:e490672012.
18	Devaraj S and Natarajan J: miRNA-mRNA network detects hub mRNAs and cancer specific miRNAs in lung cancer. In Silico Biol. 11:281–295. 2012.
19	Alshalalfa M: MicroRNA response elements-mediated miRNA-miRNA interactions in prostate cancer. Adv Bioinformatics. 2012:8398372012.
20	Arão TC, Guimarães AL, de Paula AM, Gomes CC and Gomez RS: Increased miRNA-146a and miRNA-155 expressions in oral lichen planus. Arch Dermatol Res. 304:371–375. 2012.
21	Staton AA and Giraldez AJ: Use of target protector morpholinos to analyze the physiological roles of specific miRNA-mRNA pairs in vivo. Nat Protoc. 6:2035–2049. 2011.
22	Fabbri E, Brognara E, Borgatti M, et al: miRNA therapeutics: delivery and biological activity of peptide nucleic acids targeting miRNAs. Epigenomics. 3:733–745. 2011.
23	Li SC, Liao YL, Ho MR, Tsai KW, Lai CH and Lin WC: miRNA arm selection and isomiR distribution in gastric cancer. BMC Genomics. 13(Suppl 1): S132012.
24	He M and Wang ZW: Current status and development of miRNA and siRNA research on gastric cancer. Yi Chuan. 33:925–930. 2011.(In Chinese).
25	Li C, Li JF, Cai Q, Qiu QQ, Yan M, Liu BY and Zhu ZG: miRNA-199a-3p in plasma as a potential diagnostic biomarker for gastric cancer. Ann Surg Oncol. 20(Suppl 3): S397–S405. 2013.
26	Budak H and Akpinar A: Dehydration stress-responsive miRNA in Brachypodium distachyon: evident by genome-wide screening of microRNAs expression. OMICS. 15:791–799. 2011.
27	Rao E, Jiang C, Ji M, et al: The miRNA-17 ~92 cluster mediates chemoresistance and enhances tumor growth in mantle cell lymphoma via PI3K/AKT pathway activation. Leukemia. 26:1064–1072. 2012.
28	Chen W, Tang Z, Sun Y, et al: miRNA expression profile in primary gastric cancers and paired lymph node metastases indicates that miR-10a plays a role in metastasis from primary gastric cancer to lymph nodes. Exp Ther Med. 3:351–356. 2012.
29	Tonevitsky AG, Maltseva DV, Abbasi A, Samatov TR, Sakharov DA, Shkurnikov MU, et al: Dynamically regulated miRNA-mRNA networks revealed by exercise. BMC Physiol. 13:92013.
30	Cao W, Fan R, Wang L, et al: Expression and regulatory function of miRNA-34a in targeting survivin in gastric cancer cells. Tumour Biol. 34:963–971. 2013.
31	Schnitger AK, Machova A, Mueller RU, et al: Listeria monocytogenes infection in macrophages induces vacuolar-dependent host miRNA response. PLoS One. 6:e274352011.