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Matrigel induces L‑plastin expression and promotes L‑plastin‑dependent invasion in human cholangiocarcinoma cells

  • Authors:
    • Suthidarak Chaijan
    • Sittiruk Roytrakul
    • Apiwat Mutirangura
    • Kawin Leelawat
  • View Affiliations / Copyright

    Affiliations: Inter‑Departmental Program in Biomedical Sciences, Faculty of the Graduate School, Chulalongkorn University, Bangkok 10330, Thailand, The National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Pathumtani 12120, Thailand, Center for Excellence in the Molecular Genetics of Cancer and Human Diseases, Department of Anatomy, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand, Department of Surgery, Rajavithi Hospital, Bangkok 10400, Thailand
  • Pages: 993-1000
    |
    Published online on: June 11, 2014
       https://doi.org/10.3892/ol.2014.2239
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Abstract

The function of the extracellular matrix (ECM) in the tumor microenvironment is not limited to forming a barrier against tumor invasion. As demonstrated in pathological specimens, cholangiocarcinoma samples exhibit an enrichment of the ECM surrounding the tumor cells. In this study, we examined involvement of the ECM in the regulation of the invasiveness of cholangiocarcinoma cells. The RMCCA1 cholangiocarcinoma cell line was cultured in culture plates either with or without a coating of reconstituted ECM basement membrane preparation (BD Matrigel matrix). In vitro invasion assays were then performed. In addition, the protein expression profile of the cell line was examined using two‑dimensional gel electrophoresis and liquid chromatography‑tandem mass spectrometry. The proteins expressed and their functional associations with cancer progression were determined. Culturing the RMCCA1 cell line in the BD Matrigel matrix induced cell invasion. Numerous proteins were induced by culturing the RMCCA1 cells in the matrix gel. The expression of L‑plastin, an actin‑binding protein, was significantly upregulated. The knockdown of L‑plastin expression by siRNA silencing significantly suppressed the cellular response to matrix gel‑stimulated cancer cell invasion. The ECM promotes the invasiveness of cholangiocarcinoma cells by upregulating L‑plastin. These findings suggest the potential exploitation of this mechanism as a means of inhibiting the invasiveness of cholangiocarcinoma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Chaijan S, Roytrakul S, Mutirangura A and Leelawat K: Matrigel induces L‑plastin expression and promotes L‑plastin‑dependent invasion in human cholangiocarcinoma cells. Oncol Lett 8: 993-1000, 2014.
APA
Chaijan, S., Roytrakul, S., Mutirangura, A., & Leelawat, K. (2014). Matrigel induces L‑plastin expression and promotes L‑plastin‑dependent invasion in human cholangiocarcinoma cells. Oncology Letters, 8, 993-1000. https://doi.org/10.3892/ol.2014.2239
MLA
Chaijan, S., Roytrakul, S., Mutirangura, A., Leelawat, K."Matrigel induces L‑plastin expression and promotes L‑plastin‑dependent invasion in human cholangiocarcinoma cells". Oncology Letters 8.3 (2014): 993-1000.
Chicago
Chaijan, S., Roytrakul, S., Mutirangura, A., Leelawat, K."Matrigel induces L‑plastin expression and promotes L‑plastin‑dependent invasion in human cholangiocarcinoma cells". Oncology Letters 8, no. 3 (2014): 993-1000. https://doi.org/10.3892/ol.2014.2239
Copy and paste a formatted citation
x
Spandidos Publications style
Chaijan S, Roytrakul S, Mutirangura A and Leelawat K: Matrigel induces L‑plastin expression and promotes L‑plastin‑dependent invasion in human cholangiocarcinoma cells. Oncol Lett 8: 993-1000, 2014.
APA
Chaijan, S., Roytrakul, S., Mutirangura, A., & Leelawat, K. (2014). Matrigel induces L‑plastin expression and promotes L‑plastin‑dependent invasion in human cholangiocarcinoma cells. Oncology Letters, 8, 993-1000. https://doi.org/10.3892/ol.2014.2239
MLA
Chaijan, S., Roytrakul, S., Mutirangura, A., Leelawat, K."Matrigel induces L‑plastin expression and promotes L‑plastin‑dependent invasion in human cholangiocarcinoma cells". Oncology Letters 8.3 (2014): 993-1000.
Chicago
Chaijan, S., Roytrakul, S., Mutirangura, A., Leelawat, K."Matrigel induces L‑plastin expression and promotes L‑plastin‑dependent invasion in human cholangiocarcinoma cells". Oncology Letters 8, no. 3 (2014): 993-1000. https://doi.org/10.3892/ol.2014.2239
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