Association between four common microRNA polymorphisms and the risk of hepatocellular carcinoma and HBV infection

Kou,Jian‑Tao; Fan,Hua; Han,Dongdong; Li,Lixin; Li,Ping; Zhu,Jiqiao; Ma,Jun; Zhang,Zhi‑Hua; He,Qiang

doi:10.3892/ol.2014.2257

Association between four common microRNA polymorphisms and the risk of hepatocellular carcinoma and HBV infection

Authors:
- Jian‑Tao Kou
- Hua Fan
- Dongdong Han
- Lixin Li
- Ping Li
- Jiqiao Zhu
- Jun Ma
- Zhi‑Hua Zhang
- Qiang He
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Affiliations: Department of Hepatobiliary Surgery, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, P.R. China
Published online on: June 16, 2014 https://doi.org/10.3892/ol.2014.2257
Pages: 1255-1260

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Abstract

microRNAs (miR/miRNAs) have been demonstrated to function as tumor suppressors and oncogenes, and miRNA polymorphisms may have a role in cancer development. The present study aimed to investigate the association between the miR‑146aG>C, miR‑149C>T, miR‑196a2C>T and miR‑499A>G polymorphisms and the risk of hepatocellular carcinoma (HCC) and hepatitis B virus (HBV) infection. A total of 271 patients with HCC and 532 healthy control participants were enrolled in the present study. miR‑146aG>C, miR‑149C>T, miR‑196a2C>T and miR‑499A>G polymorphisms were genotyped using the polymerase chain reaction‑restriction fragment length polymorphism method. A significant difference was identified in the genotype frequency of miR‑196a2C>T in the patients in the case group compared with the control group (χ2=6.88; P=0.032). Compared with the CC genotype, the miR‑196a2 TT genotype was associated with a significantly reduced risk of HCC [odds ratio (OR), 0.62; 95% confidence interval (CI), 0.38‑0.99], and a significantly reduced risk was also found in the dominant (OR, 0.69; 95% CI, 0.49‑0.98) and recessive (OR, 0.70; 95% CI, 0.46‑1.02) models. Moreover, individuals with HBV who were carrying the miR‑196a2 CT and TT genotypes had a significantly reduced risk of HCC (OR, 0.62; 95% CI, 0.41‑0.95; and OR, 0.39; 95% CI, 0.20‑0.73, respectively). In conclusion, the present study found that the miR‑196a2C>T polymorphism has a protective effect in patients with HCC, particularly in those with HBV infection.

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