Effect of daphnoretin on the proliferation and apoptosis of A549 lung cancer cells in vitro

Jiang,Hong‑Fang; Wu,Zhuo; Bai,Xue; Zhang,Yi; He,Ping

doi:10.3892/ol.2014.2296

Effect of daphnoretin on the proliferation and apoptosis of A549 lung cancer cells in vitro

Authors:
- Hong‑Fang Jiang
- Zhuo Wu
- Xue Bai
- Yi Zhang
- Ping He
View Affiliations

Affiliations: Department of Geriatrics, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China, Department of Thoracic Surgery, The Fourth Affiliated Hospital of China Medical University, Shenyang, Liaoning 110004, P.R. China
Published online on: June 30, 2014 https://doi.org/10.3892/ol.2014.2296
Pages: 1139-1142

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Daphnoretin is an active constituent of Wikstroemia indica C.A. Mey., which is widely distributed in the northwest and southwest regions of China. Previous studies have shown that daphnoretin has anticancer effects on leukemia, osteosarcoma and uterine cervix cancer cells. However, the effect of daphnoretin on human lung cancer cells has yet to be elucidated. In the present study, daphnoretin was observed to inhibit A549 lung cancer cell proliferation in a concentration‑ and time‑dependent manner. Fluorescent microscopy and flow cytometric analysis showed that daphnoretin induced A549 cell apoptosis in a concentration‑dependent manner. Western blot analysis also revealed that daphnoretin induced apoptosis through the regulation of the B‑cell lymphoma‑2 gene family in A549 cells. These findings indicate that daphnoretin may have potential as a therapeutic agent for the management of lung cancer.

View Figures

View References

1	Bray F, Jemal A, Grey N, Ferlay J and Forman D: Global cancer transitions according to the Human Development Index (2008–2030): a population-based study. Lancet Oncol. 13:790–801. 2012.
2	Erridge SC, Møller H, Price A and Brewster D: International comparisons of survival from lung cancer: pitfalls and warnings. Nat Clin Pract Oncol. 4:570–577. 2007.
3	Li S, Bao P, Li Z, et al: Inhibition of proliferation and apoptosis induced by a Na⁺/H⁺ exchanger-1 (NHE-1) antisense gene on drug-resistant human small cell lung cancer cells. Oncol Rep. 21:1243–1249. 2009.
4	Hsu SC, Ou CC, Chuang TC, et al: Ganoderma tsugae extract inhibits expression of epidermal growth factor receptor and angiogenesis in human epidermoid carcinoma cells: In vitro and in vivo. Cancer Lett. 281:108–116. 2009.
5	Su CC and Lin YH: Tanshinone IIA down-regulates the protein expression of ErbB-2 and up-regulates TNF-alpha in colon cancer cells in vitro and in vivo. Int J Mol Med. 22:847–851. 2008.
6	Carter BZ, Mak DH, Schober WD, et al: Triptolide sensitizes AML cells to TRAIL-induced apoptosis via decrease of XIAP and p53-mediated increase of DR5. Blood. 111:3742–3750. 2008.
7	Ko FN, Chang YL, Kuo YH, Lin YL and Teng CM: Daphnoretin, a new protein kinase C activator isolated from Wikstroemia indica C.A. Mey. Biochem J. 295:321–327. 1993.
8	Hu K, Kobayashi H, Dong A, Iwasaki S and Yao X: Antifungal, antimitotic and anti-HIV-1 agents from the roots of Wikstroemia indica. Planta Med. 66:564–567. 2000.
9	Hall IH, Tagahara K and Lee KH: Antitumor agents LIII: The effects of daphnoretin on nucleic acid and protein synthesis of Ehrlich ascites tumor cells. J Pharm Sci. 71:741–744. 1982.
10	Wang JP, Raung SL, Kuo YH and Teng CM: Daphnoretin-induced respiratory burst in rat neutrophils is, probably, mainly through protein kinase C activation. Eur J Pharmacol. 288:341–348. 1995.
11	Chen HC, Chou CK, Kuo YH and Yeh SF: Identification of a protein kinase C (PKC) activator, daphnoretin, that suppresses hepatitis B virus gene expression in human hepatoma cells. Biochem Pharmacol. 52:1025–1032. 1996.
12	Ho WS, Xue JY, Sun SS, Ooi VE and Li YL: Antiviral activity of daphnoretin isolated from Wikstroemia indica. Phytother Res. 24:657–661. 2010.
13	Lee KH, Tagahara K, Suzuki H, et al: Antitumor agents. 49 tricin, kaempferol-3-O-beta-D-glucopyranoside and (+)-nortrachelogenin, antileukemic principles from Wikstroemia indica. J Nat Prod. 44:530–535. 1981.
14	Gu S and He J: Daphnoretin induces cell cycle arrest and apoptosis in human osteosarcoma (HOS) cells. Molecules. 17:598–612. 2012.
15	Yang ZY, Kan JT, Cheng ZY, et al: Daphnoretin-induced apoptosis in HeLa cells: a possible mitochondria-dependent pathway. Cytotechnology. 66:51–61. 2014.
16	Chen CC, Lin YC, Chen YP and Hsu HYJ: A study on the constituents of Wikstroemia indica C.A. Mey. Taiwan Pharm Assoc. 33:28–29. 1981.
17	Majumder PL and Sengupta GCJ: Chemical Investigation of Daphne cannabina Wall. J Indian Chem Soc. 45:1058–1062. 1968.
18	Xiao R, Ferry AL and Dupont-Versteegden EE: Cell death-resistance of differentiated myotubes is associated with enhanced anti-apoptotic mechanisms compared to myoblasts. Apoptosis. 16:221–234. 2011.
19	Hanahan D and Weinberg RA: Hallmarks of cancer: the next generation. Cell. 144:646–674. 2011.
20	Jendrossek V: The intrinsic apoptosis pathways as a target in anticancer therapy. Curr Pharm Biotechnol. 13:1426–1438. 2012.
21	Ott M, Norberg E, Zhivotovsky B and Orrenius S: Mitochondrial targeting of tBid/Bax: a role for the TOM complex? Cell Death Differ. 16:1075–1082. 2009.
22	Robertson JD and Orrenius S: Molecular mechanisms of apoptosis induced by cytotoxic chemicals. Crit Rev Toxicol. 30:609–627. 2000.
23	Tamm I, Schriever F and Dörken B: Apoptosis: implications of basic research for clinical oncology. Lancet Oncol. 2:33–42. 2001.
24	Li SG, Wang YY, Ye ZY, et al: Proliferative and apoptotic effects of andrographolide on the BGC-823 human gastric cancer cell line. Chin Med J (Engl). 126:3739–3744. 2013.
25	Yu J, Zhou X, He X, Dai M and Zhang Q: Curcumin induces apoptosis involving bax/bcl-2 in human hepatoma SMMC-7721 cells. Asian Pac J Cancer Prev. 12:1925–1929. 2011.