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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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September-2014 Volume 8 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Inflammatory cytokines induce vascular endothelial growth factor‑C expression in melanoma‑associated macrophages and stimulate melanoma lymph node metastasis

  • Authors:
    • Silvia Peppicelli
    • Francesca Bianchini
    • Lido Calorini
  • View Affiliations / Copyright

    Affiliations: Department of Experimental and Clinical Biomedical Sciences, University of Florence, Florence 50134, Italy
  • Pages: 1133-1138
    |
    Published online on: June 30, 2014
       https://doi.org/10.3892/ol.2014.2297
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Abstract

Lymph node colonization by tumor cells is one of the key determinants of melanoma staging and prognosis, and tumor‑associated macrophages (TAMs) are the predominant type of inflammatory cell in the tumor environment which secretes vascular endothelial growth factor (VEGF)‑C, the most potent lymphangiogenic growth factor. In the present study, to elucidate the mechanism involved in VEGF‑C expression in TAMs, murine peritoneal macrophages were co‑cultivated with syngeneic B16 melanoma cells to mimic the reciprocal interactions between tumor cells and macrophages found in spontaneous tumors. In the present study, upon contact with tumor cells, macrophages were found to express a higher level of VEGF‑C which was associated with an increase in the expression of IL‑1β and TNF‑α and their receptors. Antibodies against the IL‑1β and TNF‑α receptors were added to media that had been conditioned by the macrophage‑tumor cell co‑cultures and inhibition of VEGF-C was observed in macrophages co‑cultivated with the tumor cells. Furthermore, when IL‑1β and TNF‑α were used at a non‑toxic level, they enhanced peritoneal lymph node colonization by melanoma cells. Thus, in the present study, macrophagic IL‑1β and TNF‑α were observed to promote VEGF‑C expression in TAMs, as well as melanoma lymph node metastasis, suggesting that inhibiting the signaling between tumor cells and TAMs may be required to inhibit lymphangiogenesis and lymph node metastasis.
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Copy and paste a formatted citation
Spandidos Publications style
Peppicelli S, Bianchini F and Calorini L: Inflammatory cytokines induce vascular endothelial growth factor‑C expression in melanoma‑associated macrophages and stimulate melanoma lymph node metastasis. Oncol Lett 8: 1133-1138, 2014.
APA
Peppicelli, S., Bianchini, F., & Calorini, L. (2014). Inflammatory cytokines induce vascular endothelial growth factor‑C expression in melanoma‑associated macrophages and stimulate melanoma lymph node metastasis. Oncology Letters, 8, 1133-1138. https://doi.org/10.3892/ol.2014.2297
MLA
Peppicelli, S., Bianchini, F., Calorini, L."Inflammatory cytokines induce vascular endothelial growth factor‑C expression in melanoma‑associated macrophages and stimulate melanoma lymph node metastasis". Oncology Letters 8.3 (2014): 1133-1138.
Chicago
Peppicelli, S., Bianchini, F., Calorini, L."Inflammatory cytokines induce vascular endothelial growth factor‑C expression in melanoma‑associated macrophages and stimulate melanoma lymph node metastasis". Oncology Letters 8, no. 3 (2014): 1133-1138. https://doi.org/10.3892/ol.2014.2297
Copy and paste a formatted citation
x
Spandidos Publications style
Peppicelli S, Bianchini F and Calorini L: Inflammatory cytokines induce vascular endothelial growth factor‑C expression in melanoma‑associated macrophages and stimulate melanoma lymph node metastasis. Oncol Lett 8: 1133-1138, 2014.
APA
Peppicelli, S., Bianchini, F., & Calorini, L. (2014). Inflammatory cytokines induce vascular endothelial growth factor‑C expression in melanoma‑associated macrophages and stimulate melanoma lymph node metastasis. Oncology Letters, 8, 1133-1138. https://doi.org/10.3892/ol.2014.2297
MLA
Peppicelli, S., Bianchini, F., Calorini, L."Inflammatory cytokines induce vascular endothelial growth factor‑C expression in melanoma‑associated macrophages and stimulate melanoma lymph node metastasis". Oncology Letters 8.3 (2014): 1133-1138.
Chicago
Peppicelli, S., Bianchini, F., Calorini, L."Inflammatory cytokines induce vascular endothelial growth factor‑C expression in melanoma‑associated macrophages and stimulate melanoma lymph node metastasis". Oncology Letters 8, no. 3 (2014): 1133-1138. https://doi.org/10.3892/ol.2014.2297
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