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October 2014 Volume 8 Issue 4

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Article

Regulatory roles of microRNA‑708 and microRNA‑31 in proliferation, apoptosis and invasion of colorectal cancer cells

  • Authors:
    • San-Lin Lei
    • Hua Zhao
    • Hong-Liang Yao
    • Yong Chen
    • Zhen-Dong Lei
    • Kui-Jie Liu
    • Qun Yang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China, Department of Oncology, The Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, P.R. China
  • Pages: 1768-1774
    |
    Published online on: July 9, 2014
       https://doi.org/10.3892/ol.2014.2328
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Abstract

MicroRNAs (miRs) function as key regulators of gene expression and their deregulation is associated with the carcinogenesis of various cancers. In the present study, the aim was to validate the potential roles and regulatory mechanisms of miR‑708 and miR‑31 in colorectal cancer (CRC) cells. miR‑708 and miR‑31 were found to be highly expressed in five CRC tissue samples. Functional studies showed that the inhibition of miR‑708 and miR‑31 inhibited cell proliferation and invasion, however, promoted apoptosis in vitro. Subsequently, it was identified that miR‑708 and miR‑31 directly target cyclin‑dependent kinase inhibitor 2B (CDKN2B) by binding to the 3' untranslated region, which suppresses the CDKN2B protein levels. In addition, the CDKN2B protein levels were significantly reduced when there was high miR‑708 and miR‑31 expression in the CRC tissue samples. The results indicate that miR‑31 and miR‑708 function in an oncogenic manner in CRC development, and inhibition of the two miRs may be used as a therapeutic strategy for patients with CRC.
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Copy and paste a formatted citation
Spandidos Publications style
Lei S, Zhao H, Yao H, Chen Y, Lei Z, Liu K and Yang Q: Regulatory roles of microRNA‑708 and microRNA‑31 in proliferation, apoptosis and invasion of colorectal cancer cells. Oncol Lett 8: 1768-1774, 2014.
APA
Lei, S., Zhao, H., Yao, H., Chen, Y., Lei, Z., Liu, K., & Yang, Q. (2014). Regulatory roles of microRNA‑708 and microRNA‑31 in proliferation, apoptosis and invasion of colorectal cancer cells. Oncology Letters, 8, 1768-1774. https://doi.org/10.3892/ol.2014.2328
MLA
Lei, S., Zhao, H., Yao, H., Chen, Y., Lei, Z., Liu, K., Yang, Q."Regulatory roles of microRNA‑708 and microRNA‑31 in proliferation, apoptosis and invasion of colorectal cancer cells". Oncology Letters 8.4 (2014): 1768-1774.
Chicago
Lei, S., Zhao, H., Yao, H., Chen, Y., Lei, Z., Liu, K., Yang, Q."Regulatory roles of microRNA‑708 and microRNA‑31 in proliferation, apoptosis and invasion of colorectal cancer cells". Oncology Letters 8, no. 4 (2014): 1768-1774. https://doi.org/10.3892/ol.2014.2328
Copy and paste a formatted citation
x
Spandidos Publications style
Lei S, Zhao H, Yao H, Chen Y, Lei Z, Liu K and Yang Q: Regulatory roles of microRNA‑708 and microRNA‑31 in proliferation, apoptosis and invasion of colorectal cancer cells. Oncol Lett 8: 1768-1774, 2014.
APA
Lei, S., Zhao, H., Yao, H., Chen, Y., Lei, Z., Liu, K., & Yang, Q. (2014). Regulatory roles of microRNA‑708 and microRNA‑31 in proliferation, apoptosis and invasion of colorectal cancer cells. Oncology Letters, 8, 1768-1774. https://doi.org/10.3892/ol.2014.2328
MLA
Lei, S., Zhao, H., Yao, H., Chen, Y., Lei, Z., Liu, K., Yang, Q."Regulatory roles of microRNA‑708 and microRNA‑31 in proliferation, apoptosis and invasion of colorectal cancer cells". Oncology Letters 8.4 (2014): 1768-1774.
Chicago
Lei, S., Zhao, H., Yao, H., Chen, Y., Lei, Z., Liu, K., Yang, Q."Regulatory roles of microRNA‑708 and microRNA‑31 in proliferation, apoptosis and invasion of colorectal cancer cells". Oncology Letters 8, no. 4 (2014): 1768-1774. https://doi.org/10.3892/ol.2014.2328
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