Dihydromyricetin induces apoptosis and inhibits proliferation in hepatocellular carcinoma cells

Liu,Jie; Shu,Yang; Zhang,Qingyu; Liu,Bin; Xia,Juan; Qiu,Mingning; Miao,Huilai; Li,Mingyi; Zhu,Runzhi

doi:10.3892/ol.2014.2330

October 2014 Volume 8 Issue 4

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October 2014 Volume 8 Issue 4

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Dihydromyricetin induces apoptosis and inhibits proliferation in hepatocellular carcinoma cells

Authors:
- Jie Liu
- Yang Shu
- Qingyu Zhang
- Bin Liu
- Juan Xia
- Mingning Qiu
- Huilai Miao
- Mingyi Li
- Runzhi Zhu
View Affiliations / Copyright

Affiliations: Zhanjiang Key Laboratory of Hepatobiliary Diseases, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Gunagdong 524001, P.R. China, Department of Urology Surgery, Affiliated Hospital of Guangdong Medical College, Zhanjiang, Gunagdong 524001, P.R. China
Pages: 1645-1651
|
Published online on: July 9, 2014

https://doi.org/10.3892/ol.2014.2330
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Abstract

Hepatocellular carcinoma (HCC) is a life‑threatening disease that is known to exhibit a poor prognosis. Therefore, it is important to identify an effective drug therapy for the treatment of HCC. Dihydromyricetin (DHM) is a flavonoid compound, isolated from the classical Chinese herb Ampelopsis grossedentata, which exhibits multiple pharmacological activities, including anticancer effects. In this study, the anticancer effect of DHM was investigated in nine different types of HCC cell lines via cell proliferation and immunoassays, as well as apoptosis detection. Two immortalized normal human liver cell lines were utilized to determine hepatotoxicity. The results revealed that DHM significantly inhibited cell proliferation and induced cell apoptosis in the HCC cell lines. However, DHM exhibited no cytotoxicity to normal human hepatic cell lines. Furthermore, it was found that DHM induced cell apoptosis in a p53‑dependent manner. DHM upregulated p53 expression, and the upregulation of p53 increased the levels of the cleaved caspase‑3 protein, directly inducing cell apoptosis. These results indicate that DHM is a promising candidate for the treatment of HCC.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Dihydromyricetin induces apoptosis and inhibits proliferation in hepatocellular carcinoma cells

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