Effect of CIK on multidrug‑resistance reversal and increasing the sensitivity of ADR in K562/ADR cells

Wang,Lei; Deng,Qi; Wang,Jian; Bai,Xue; Xiao,Xia; Lv,Hai‑Rong; Zhao,Ming‑Feng; Liu,Peng‑Jiang

doi:10.3892/ol.2014.2337

October 2014 Volume 8 Issue 4

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October 2014 Volume 8 Issue 4

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Article

Effect of CIK on multidrug‑resistance reversal and increasing the sensitivity of ADR in K562/ADR cells

Authors:
- Lei Wang
- Qi Deng
- Jian Wang
- Xue Bai
- Xia Xiao
- Hai‑Rong Lv
- Ming‑Feng Zhao
- Peng‑Jiang Liu
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Affiliations: International Medical Center, The First Central Hospital of Tianjin, Tianjin 300192, P.R. China, Department of Hematology, The First Central Hospital of Tianjin, Tianjin 300192, P.R. China
Pages: 1778-1782
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Published online on: July 10, 2014

https://doi.org/10.3892/ol.2014.2337
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Abstract

Leukemia is a leading cause of cancer‑related mortality in children worldwide, and multidrug‑resistance (MDR) is a main reason for tumor chemotherapy failure. The present study investigated the effects of ADR following incubation with cytokine‑induced killer (CIK) cells on reversing MDR in K562/ADR cells. Mononuclear cells were isolated from the peripheral blood of healthy individuals and cultured in vitro in the presence of a combination of cytokines to generate CIK for K562/ADR cell treatment. A decreased level of P‑glycoprotein expression and glutathione (GSH), an increased intracellular Rh‑123 content, decreased mRNA and protein expression levels of MDR gene 1, MDR‑associated protein 1, GSH S‑transferase‑π, B-cell lymphoma 2 and Survivin, and the decreased phosphorylation of AKT and the transcriptional activity of nuclear factor‑κB and activator protein 1 were detected following ADR treatment in CIK co‑cultured K562/ADR cells. Additionally, the level of ADR sensitivity and the apoptosis rate were increased in the CIK co‑cultured K562/ADR cells. These results indicate that pre‑treatment with CIK could reverse the MDR of K562/ADR cells, and that patients would be most likely to benefit from the combination of chemotherapy and CIK therapy.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Effect of CIK on multidrug‑resistance reversal and increasing the sensitivity of ADR in K562/ADR cells

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