Effect of CIK on multidrug‑resistance reversal and increasing the sensitivity of ADR in K562/ADR cells
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- Published online on: July 10, 2014 https://doi.org/10.3892/ol.2014.2337
- Pages: 1778-1782
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Abstract
Leukemia is a leading cause of cancer‑related mortality in children worldwide, and multidrug‑resistance (MDR) is a main reason for tumor chemotherapy failure. The present study investigated the effects of ADR following incubation with cytokine‑induced killer (CIK) cells on reversing MDR in K562/ADR cells. Mononuclear cells were isolated from the peripheral blood of healthy individuals and cultured in vitro in the presence of a combination of cytokines to generate CIK for K562/ADR cell treatment. A decreased level of P‑glycoprotein expression and glutathione (GSH), an increased intracellular Rh‑123 content, decreased mRNA and protein expression levels of MDR gene 1, MDR‑associated protein 1, GSH S‑transferase‑π, B-cell lymphoma 2 and Survivin, and the decreased phosphorylation of AKT and the transcriptional activity of nuclear factor‑κB and activator protein 1 were detected following ADR treatment in CIK co‑cultured K562/ADR cells. Additionally, the level of ADR sensitivity and the apoptosis rate were increased in the CIK co‑cultured K562/ADR cells. These results indicate that pre‑treatment with CIK could reverse the MDR of K562/ADR cells, and that patients would be most likely to benefit from the combination of chemotherapy and CIK therapy.
