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Elevated p53 expression levels correlate with tumor progression and poor prognosis in patients exhibiting esophageal squamous cell carcinoma

  • Authors:
    • Kate Huang
    • Lin Chen
    • Jiliang Zhang
    • Zhi Wu
    • Linhua  Lan
    • Lu Wang
    • Bin Lu
    • Yongzhang Liu
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, Department of Biochemistry and Molecular Biology, Attardi Institute of Mitochondrial Biomedicine, Zhejiang Provincial Key Laboratory of Medical Genetics, School of Laboratory Medicine and Life Sciences, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China
  • Pages: 1441-1446
    |
    Published online on: July 11, 2014
       https://doi.org/10.3892/ol.2014.2343
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Abstract

Esophageal squamous cell carcinoma (ESCC) is the most common histological subtype of esophageal cancer and one of the most aggressive types of malignancy, with a high rate of mortality. Early diagnosis and treatment may improve the prognosis of ESCC and, thus, survival rates. As a significant tumor suppressor, p53 is closely associated with apoptosis and the differentiation of cancer cells. The present study evaluated the expression levels of the p53 protein and the clinical significance in patients presenting with ESCC. The p53 protein expression level of 64 paired ESCC and tumor‑adjacent normal tissues was evaluated using western blot analysis. In addition, immunohistochemistry (IHC) was performed to detect the p53 expression level in specimens from 118 paraffin‑embedded cancerous tissues. The correlation of the p53 expression level with the clinicopathological parameters and prognosis of the ESCC patients was also analyzed. The p53 protein was identified to be highly expressed in the ESCC tissue, with western blot analysis demonstrating that the expression level of p53 in the cancerous tissue was 1.89 times that of the tumor‑adjacent normal tissue (P<0.001); furthermore, IHC indicated that there was a marked positive expression of p53 in the ESCC tissue (49.15%). The expression level of p53 protein was identified to be significantly correlated with the tumor grade (P<0.001), N stage (P=0.010). Additionally, the higher level of p53 expression was found to be associated with a poor survival rate in the ESCC patients (P=0.0404). The univariate analysis showed that the survival time of patients was significantly correlated with the T stage (RR=3.886, P<0.001), N stage (lymph node metastasis; RR=3.620, P<0.001) and TNM stage (RR=3.576, P<0.001). Furthermore, the multivariate analysis revealed that the T stage (RR=3.988, P<0.001) and N stage (RR=4.240, P=0.004) significantly influenced the overall survival of the ESCC patients.
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Copy and paste a formatted citation
Spandidos Publications style
Huang K, Chen L, Zhang J, Wu Z, Lan L, Wang L, Lu B and Liu Y: Elevated p53 expression levels correlate with tumor progression and poor prognosis in patients exhibiting esophageal squamous cell carcinoma. Oncol Lett 8: 1441-1446, 2014.
APA
Huang, K., Chen, L., Zhang, J., Wu, Z., Lan, L., Wang, L. ... Liu, Y. (2014). Elevated p53 expression levels correlate with tumor progression and poor prognosis in patients exhibiting esophageal squamous cell carcinoma. Oncology Letters, 8, 1441-1446. https://doi.org/10.3892/ol.2014.2343
MLA
Huang, K., Chen, L., Zhang, J., Wu, Z., Lan, L., Wang, L., Lu, B., Liu, Y."Elevated p53 expression levels correlate with tumor progression and poor prognosis in patients exhibiting esophageal squamous cell carcinoma". Oncology Letters 8.4 (2014): 1441-1446.
Chicago
Huang, K., Chen, L., Zhang, J., Wu, Z., Lan, L., Wang, L., Lu, B., Liu, Y."Elevated p53 expression levels correlate with tumor progression and poor prognosis in patients exhibiting esophageal squamous cell carcinoma". Oncology Letters 8, no. 4 (2014): 1441-1446. https://doi.org/10.3892/ol.2014.2343
Copy and paste a formatted citation
x
Spandidos Publications style
Huang K, Chen L, Zhang J, Wu Z, Lan L, Wang L, Lu B and Liu Y: Elevated p53 expression levels correlate with tumor progression and poor prognosis in patients exhibiting esophageal squamous cell carcinoma. Oncol Lett 8: 1441-1446, 2014.
APA
Huang, K., Chen, L., Zhang, J., Wu, Z., Lan, L., Wang, L. ... Liu, Y. (2014). Elevated p53 expression levels correlate with tumor progression and poor prognosis in patients exhibiting esophageal squamous cell carcinoma. Oncology Letters, 8, 1441-1446. https://doi.org/10.3892/ol.2014.2343
MLA
Huang, K., Chen, L., Zhang, J., Wu, Z., Lan, L., Wang, L., Lu, B., Liu, Y."Elevated p53 expression levels correlate with tumor progression and poor prognosis in patients exhibiting esophageal squamous cell carcinoma". Oncology Letters 8.4 (2014): 1441-1446.
Chicago
Huang, K., Chen, L., Zhang, J., Wu, Z., Lan, L., Wang, L., Lu, B., Liu, Y."Elevated p53 expression levels correlate with tumor progression and poor prognosis in patients exhibiting esophageal squamous cell carcinoma". Oncology Letters 8, no. 4 (2014): 1441-1446. https://doi.org/10.3892/ol.2014.2343
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