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Article

Hsa‑microRNA‑101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells

  • Authors:
    • Chenghai Wang
    • Sijia Lu
    • Jixin Jiang
    • Xiaoqin Jia
    • Xiaoyun Dong
    • Ping Bu
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Medical College of Yangzhou University, Yangzhou, Jiangshu 225001, P.R. China, Department of Pathology, The Affiliated Jiangsu Subei Hospital, Yangzhou University, Yangzhou, Jiangshu 225001, P.R. China, Department of Chinese and Western Integrative Medicine, Medical College of Yangzhou University, Yangzhou, Jiangshu 225001, P.R. China
  • Pages: 1815-1821
    |
    Published online on: July 18, 2014
       https://doi.org/10.3892/ol.2014.2361
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Abstract

MicroRNAs (miRNAs) are 22‑ to 25‑nucleotide non‑coding RNA molecules that function as negative regulators of gene expression. In previous years, increasing evidence has arisen implicating the involvement of miRNAs in carcinogenesis. In previous studies, the role of miRNA‑101 (miR‑101) in tumors has been identified as a tumor suppressor and, until now, the role of miR‑101 and Rac1 in thyroid cancer has remained undefined. This study revealed that miR‑101 is significantly downregulated in papillary thyroid carcinoma (PTC) tissue and thyroid cancer cell lines, and that the downregulated miR‑101 is associated with lymph node metastasis. Infection with the miR‑101 murine stem cell virus may markedly inhibit cell migration and invasion in TPC‑1 and HTH83 thyroid cancer cell lines. Rac1 was demonstrated to be negatively regulated by miR‑101 at the post‑transcriptional level, via a specific target site within the 3' untranslated region by dual‑luciferase reporter assay. The expression of Rac1 was also observed to inversely correlate with miR‑101 expression in PTC tissues; knockdown of Rac1 by shRNA inhibited thyroid cancer cell migration and invasion, resembling that of miR‑101 overexpression. Thus, these findings suggested that miR‑101 acts as a novel suppressor by targeting the Rac1 gene and inhibiting thyroid cancer cell migration and invasion.
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Copy and paste a formatted citation
Spandidos Publications style
Wang C, Lu S, Jiang J, Jia X, Dong X and Bu P: Hsa‑microRNA‑101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells. Oncol Lett 8: 1815-1821, 2014.
APA
Wang, C., Lu, S., Jiang, J., Jia, X., Dong, X., & Bu, P. (2014). Hsa‑microRNA‑101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells. Oncology Letters, 8, 1815-1821. https://doi.org/10.3892/ol.2014.2361
MLA
Wang, C., Lu, S., Jiang, J., Jia, X., Dong, X., Bu, P."Hsa‑microRNA‑101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells". Oncology Letters 8.4 (2014): 1815-1821.
Chicago
Wang, C., Lu, S., Jiang, J., Jia, X., Dong, X., Bu, P."Hsa‑microRNA‑101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells". Oncology Letters 8, no. 4 (2014): 1815-1821. https://doi.org/10.3892/ol.2014.2361
Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Lu S, Jiang J, Jia X, Dong X and Bu P: Hsa‑microRNA‑101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells. Oncol Lett 8: 1815-1821, 2014.
APA
Wang, C., Lu, S., Jiang, J., Jia, X., Dong, X., & Bu, P. (2014). Hsa‑microRNA‑101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells. Oncology Letters, 8, 1815-1821. https://doi.org/10.3892/ol.2014.2361
MLA
Wang, C., Lu, S., Jiang, J., Jia, X., Dong, X., Bu, P."Hsa‑microRNA‑101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells". Oncology Letters 8.4 (2014): 1815-1821.
Chicago
Wang, C., Lu, S., Jiang, J., Jia, X., Dong, X., Bu, P."Hsa‑microRNA‑101 suppresses migration and invasion by targeting Rac1 in thyroid cancer cells". Oncology Letters 8, no. 4 (2014): 1815-1821. https://doi.org/10.3892/ol.2014.2361
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