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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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October 2014 Volume 8 Issue 4

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International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line‑derived exosomes

  • Authors:
    • Zhuoyuan Zhang
    • Chenxing Wang
    • Tang Li
    • Zhe Liu
    • Longjiang Li
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan University, Chengdu, Sichuan 610041, P.R. China
  • Pages: 1701-1706
    |
    Published online on: July 23, 2014
       https://doi.org/10.3892/ol.2014.2373
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Abstract

The aim of the present study was to compare the method of ultracentrifugation and density gradient separation for isolating Tca8113 human tongue squamous cell carcinoma cell line‑derived exosomes. The exosomes were obtained from the culture supernatant of cultured Tca8113 cells, respectively, followed by identification with transmission electron microscopy observation and western blot analysis. The two different methods were then compared by the morphology, the distribution range of the particle size and the concentration of proteins of the extracted exosomes. In vitro, Tca8113 cells can secrete a large amount of vesicle‑like structures, which are identified as exosomes by the presence of the surface markers, Hsp‑70 and Alix. The protein profile of the two products are almost the same, however the particle size distribution of the exosomes extracted with density gradient centrifugation are more limited, between 40‑120 nm, and these have a higher protein concentration. The results indicate that Tca8113 cells can secrete exosomes in vitro, and the density gradient separation methods for purifying exosomes is improved, which is helpful for future research and application of exosomes.
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1 

Johnstone RM, Adam M, Hammond JR, et al: Vesicle formation during reticulocyte maturation. Association of plasma membrane activities with released vesicles (exosomes). J Biol Chem. 262:9412–9420. 1987.

2 

Cocucci E, Racchetti G and Meldolesi J: Shedding microvesicles: artefacts no more. Trends Cell Biol. 19:43–51. 2009.

3 

Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, et al: Large-scale proteomics and phosphoproteomics of urinary exosomes. J Am Soc Nephrol. 20:363–379. 2009.

4 

Nilsson J, Skog J, Nordstrand A, Baranov V, Mincheva-Nilsson L, Breakefield XO and Widmark A: Prostate cancer-derived urine exosomes: a novel approach to biomarkers for prostate cancer. Br J Cancer. 100:1603–1607. 2009.

5 

Michael A, Bajracharya SD, Yuen PS, Zhou H, Star RA, Illei GG and Alevizos I: Exosomes from human saliva as a source of microRNA biomarkers. Oral Dis. 16:34–38. 2010.

6 

Skog J, Würdinger T, van Rijn S, et al: Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol. 10:1470–1476. 2008.

7 

Hong BS, Cho JH, Kim H, et al: Colorectal cancer cell-derived microvesicles are enriched in cell cycle-related mRNAs that promote proliferation of endothelial cells. BMC Genomics. 10:5562009.

8 

Zhang HG and Grizzle WE: Exosomes and cancer: a newly described pathway of immune suppression. Clin Cancer Res. 17:959–964. 2011.

9 

Keller S, Sanderson MP, Stoeck A and Altevogt P: Exosomes: from biogenesis and secretion to biological function. Immunol Lett. 107:102–108. 2006.

10 

Simons M and Raposo G: Exosomes - vesicular carriers for intercellular communication. Curr Opp Cell Biol. 21:575–581. 2009.

11 

Mathivanan S, Ji H and Simpson RJ: Exosomes: extracellular organelles important in intercellular communication. J Proteomics. 73:1907–1920. 2010.

12 

Raposo G, Nijman HW, Stoorvogel W, Liejendekker R, Harding CV, Melief CJ and Geuze HJ: B lymphocytes secrete antigen-presenting vesicles. J Exp Med. 183:1161–1172. 1996.

13 

Ostrowski M, Carmo NB, Krumeich S, et al: Rab27a and Rab27b control different steps of the exosome secretion pathway. Nat Cell Biol. 12:19–30. 2010.

14 

Rabinowits G, Gerçel-Taylor C, Day JM, Taylor DD and Kloecker GH: Exosomal microRNA: a diagnostic marker for lung cancer. Clin Lung Cancer. 10:42–46. 2009.

15 

Taylor DD and Gercel-Taylor C: MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecol Oncol. 110:13–21. 2008.

16 

Mathivanan S, Lim JW, Tauro BJ, Ji H, Moritz RL and Simpson RJ: Proteomics analysis of A33 immunoaffinity-purified exosomes released from the human colon tumor cell line LIM1215 reveals a tissue-specific protein signature. Mol Cell Proteomics. 9:197–208. 2010.

17 

Lamparski HG, Metha-Damani A, Yao JY, Patel S, Hsu DH, Ruegg C and Le Pecq JB: Production and characterization of clinical grade exosomes derived from dendritic cells. J Immunol Methods. 270:211–226. 2002.

18 

Chairoungdua A, Smith DL, Pochard P, Hull M and Caplan MJ: Exosome release of beta-catenin: a novel mechanism that antagonizes Wnt signalling. J Cell Biol. 190:1079–1091. 2010.

19 

Verweij FJ, van Eijndhoven MA, Hopmans ES, et al: LMP1 association with CD63 in endosomes and secretion via exosomes limits constitutive NF-kappaB activation. EMBO J. 30:2115–2129. 2011.

20 

Ohshima K, Inoue K, Fujiwara A, et al: Let-7 microRNA family is selectively secreted into the extracellular environment via exosomes in a metastatic gastric cancer cell line. PloS One. 5:e132472010.

21 

Clayton A, Al-Taei S, Webber J, Mason MD and Tabi Z: Cancer exosomes express CD39 and CD73, which suppress T cells through adenosine production. J Immunol. 187:676–683. 2011.

22 

Xiang X, Poliakov A, Liu C, et al: Induction of myeloid-derived suppressor cells by tumor exosomes. Int J Cancer. 124:2621–2633. 2009.

23 

Castellana D, Zobairi F, Martinez MC, et al: Membrane microvesicles as actors in the establishment of a favorable prostatic tumoral niche: a role for activated fibroblasts and CX3CL1-CX3CR1 axis. Cancer Res. 69:785–793. 2009.

24 

Xiao WH, Sanren GW, Zhu JH, et al: Effect of 5-aza-2′-deoxycytidine on immune-associated proteins in exosomes from hepatoma. World J Gastroenterol. 16:2371–2377. 2010.

25 

Regev-Rudzki N, Wilson DW, Carvalho TG, et al: Cell-cell communication between malaria-infected red blood cells via exosome-like vesicles. Cell. 153:1120–1133. 2013.

26 

Lamparski HG, Metha-Damani A, Yao JY, Patel S, Hsu DU, Ruegg C and LePecq JB: Production and characterization of clinical grade exosomes derived from dendritic cells. J Immunol Methods. 270:211–226. 2002.

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Copy and paste a formatted citation
Spandidos Publications style
Zhang Z, Wang C, Li T, Liu Z and Li L: Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line‑derived exosomes. Oncol Lett 8: 1701-1706, 2014.
APA
Zhang, Z., Wang, C., Li, T., Liu, Z., & Li, L. (2014). Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line‑derived exosomes. Oncology Letters, 8, 1701-1706. https://doi.org/10.3892/ol.2014.2373
MLA
Zhang, Z., Wang, C., Li, T., Liu, Z., Li, L."Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line‑derived exosomes". Oncology Letters 8.4 (2014): 1701-1706.
Chicago
Zhang, Z., Wang, C., Li, T., Liu, Z., Li, L."Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line‑derived exosomes". Oncology Letters 8, no. 4 (2014): 1701-1706. https://doi.org/10.3892/ol.2014.2373
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Z, Wang C, Li T, Liu Z and Li L: Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line‑derived exosomes. Oncol Lett 8: 1701-1706, 2014.
APA
Zhang, Z., Wang, C., Li, T., Liu, Z., & Li, L. (2014). Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line‑derived exosomes. Oncology Letters, 8, 1701-1706. https://doi.org/10.3892/ol.2014.2373
MLA
Zhang, Z., Wang, C., Li, T., Liu, Z., Li, L."Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line‑derived exosomes". Oncology Letters 8.4 (2014): 1701-1706.
Chicago
Zhang, Z., Wang, C., Li, T., Liu, Z., Li, L."Comparison of ultracentrifugation and density gradient separation methods for isolating Tca8113 human tongue cancer cell line‑derived exosomes". Oncology Letters 8, no. 4 (2014): 1701-1706. https://doi.org/10.3892/ol.2014.2373
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