Ptaquiloside‑induced cytotoxicity in Crandall feline kidney and HGC‑27 cells

Yurdakok,Begum; Kismali,Gorkem; Ozen,Dogukan

doi:10.3892/ol.2014.2378

Ptaquiloside‑induced cytotoxicity in Crandall feline kidney and HGC‑27 cells

Authors:
- Begum Yurdakok
- Gorkem Kismali
- Dogukan Ozen
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Affiliations: Department of Pharmacology and Toxicology, Ankara University Faculty of Veterinary Medicine, Ankara 06110, Turkey, Department of Biochemistry, Ankara University Faculty of Veterinary Medicine, Ankara 06110, Turkey, Department of Biostatistics, Ankara University Faculty of Veterinary Medicine, Ankara 06110, Turkey
Published online on: July 24, 2014 https://doi.org/10.3892/ol.2014.2378
Pages: 1839-1843

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Abstract

Ptaquiloside (PTA) is a potent genotoxic carcinogenic compound, which is found in bracken ferns and predominantly causes gastric tumors in humans, as well as bladder tumors and chronic enzootic hematuria in cattle. The underlying molecular mechanisms of PTA remain a topic for interdisciplinary investigation. The aim of the present study was to determine the possible cytotoxic effect of 24 h of PTA exposure in Crandall feline kidney (CrFK) and human gastric cells (the HGC‑27 cell line) using the 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide (MTT) assay and lactose dehydrogenase (LDH) analysis. The cytotoxic effects of PTA (0.0005‑500 µg/ml) were found to increase in a dose‑dependent manner, whereby the half maximal inhibitory concentration values were 11.17 and 11.86 µg/ml in the CrFK cells, and 2.03 and 2.56 µg/ml in the HGC‑27 cells, by LDH and MTT assay, respectively. The results of the present study are consistent with those of previous studies associated with the cytotoxicity of PTA; however, cytotoxicity was identified to occur at significantly lower doses. This cytotoxic effect in vitro at particularly high doses may be linked to the initiation of carcinogenesis as a result of oxidative stress.

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