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Reduced expression of autophagy markers correlates with high‑risk human papillomavirus infection in human cervical squamous cell carcinoma

  • Authors:
    • Hua‑Yi Wang
    • Gui‑Fang Yang
    • Yan‑Hua Huang
    • Qi‑Wen Huang
    • Jun Gao
    • Xian‑Da Zhao
    • Li‑Ming Huang
    • Hong‑Lei Chen
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Jingzhou Second People's Hospital, Jingzhou, Hubei 434000, P.R. China, Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Molecular Pathology, Wuhan Nano Tumor Diagnosis Engineering Research Center, Wuhan, Hubei 430075, P.R. China, Department of Pathology, School of Basic Medical Science, Wuhan University, P.R. China, Department of Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
  • Pages: 1492-1498
    |
    Published online on: August 5, 2014
       https://doi.org/10.3892/ol.2014.2417
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Abstract

Infection by an oncogenic human papillomavirus (HPV), in particular HPV16 and 18, is a high risk factor for developing cervical cancer; however, viral infection alone is not sufficient for cancer progression. Autophagy is hypothesized to be an important process during carcinogenesis. The aim of the present study was to investigate the association between autophagy and high‑risk HPV (hrHPV) infection in human cervical squamous cell carcinomas (SCCs), and to analyze the clinical significance of this association. Quantum dot (QD)‑based immunofluorescence histochemistry was used to detect the expression of autophagy markers, Beclin‑1 and microtubule‑associated proteins 1A/1B light chain 3B (LC3B) proteins, in 104 cases of cervical cancer (including 80 SCCs and 24 adenocarcinomas) and 20 normal cervical tissues. hrHPV (HPV16/18) infection was detected by QDs based fluorescence in situ hybridization in cervical cancers. The results revealed that the expression levels of Beclin‑1 and LC3B were significantly lower in cervical cancer cells when compared with those of normal cervical squamous epithelial cells, and were found to negatively correlate with hrHPV infection. The expression levels of Beclin‑1 and LC3B were not associated with age, tumor grade, tumor stage, tumor node metastasis stage or lymph node metastasis. However, a positive correlation was identified between Beclin‑1 and LC3B protein expression. In addition, the absence of autophagy in combination with hrHPV infection may accelerate the progression of cervical SCC. In conclusion, decreased expression of Beclin‑1 and LC3B may be important in cervical carcinogenesis. The hrHPV‑host cell interaction may inhibit autophagy, which may aid virus duplication and infection, as well as cervical cancer development.
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1 

Pandey S, Mishra M and Chandrawati: Human papillomavirus screening in north Indian women. Asian Pac J Cancer Prev. 13:2643–2646. 2012.

2 

Walboomers JM, Jacobs MV, Manos MM, et al: Human papillomavirus is a necessary cause of invasive cervical cancer worldwide. J Pathol. 189:12–19. 1999.

3 

Li J, Kang LN and Qiao YL: Review of the cervical cancer disease burden in mainland China. Asian Pac J Cancer Prev. 12:1149–1153. 2011.

4 

Muñoz N: Human papillomavirus and cancer: the epidemiological evidence. J Clin Virol. 19:1–5. 2000.

5 

zur Hausen H: Papillomaviruses and cancer: from basic studies to clinical application. Nat Rev Cancer. 2:342–350. 2002.

6 

Nakatogawa H, Suzuki K, Kamada Y and Ohsumi Y: Dynamics and diversity in autophagy mechanisms: lessons from yeast. Nat Rev Mol Cell Biol. 10:458–467. 2009.

7 

Liu JJ, Lin M, Yu JY, Liu B and Bao JK: Targeting apoptotic and autophagic pathways for cancer therapeutics. Cancer Lett. 300:105–114. 2011.

8 

Janku F, McConkey DJ, Hong DS and Kurzrock R: Autophagy as a target for anticancer therapy. Nat Rev Clin Oncol. 8:528–539. 2011.

9 

Mathew R and White E: Autophagy in tumorigenesis and energy metabolism: friend by day, foe by night. Curr Opin Genet Dev. 21:113–119. 2011.

10 

Mizushima N: The pleiotropic role of autophagy: from protein metabolism to bactericide. Cell Death Differ. 12(Suppl 2): S1535–S1541. 2005.

11 

Chen HL, Peng J, Zhu XB, et al: Detection of EBV in nasopharyngeal carcinoma by quantum dot fluorescent in situ hybridization. Exp Mol Pathol. 89:367–371. 2010.

12 

Eskelinen EL and Saftig P: Autophagy: a lysosomal degradation pathway with a central role in health and disease. Biochim Biophys Acta. 1793:664–673. 2009.

13 

Miracco C, Cevenini G, Franchi A, et al: Beclin 1 and LC3 autophagic gene expression in cutaneous melanocytic lesions. Hum Pathol. 41:503–512. 2010.

14 

Zhang YH, Wu YL, Tashiro S, Onodera S and Ikejima T: Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells. Acta Pharmacol Sin. 32:1266–1275. 2011.

15 

Wang ZH, Xu L, Wang Y, Cao MQ, Li L and Bai T: Clinicopathologic correlations between human papillomavirus 16 infection and Beclin 1 expression in human cervical cancer. Int J Gynecol Pathol. 30:400–406. 2011.

16 

Pecorelli S: Revised FIGO staging for carcinoma of the vulva, cervix, and endometrium. Int J Gynaecol Obstet. 105:103–104. 2009.

17 

Li M, Chen H, Diao L, Zhang Y, Xia C and Yang F: Caveolin-1 and VEGF-C promote lymph node metastasis in the absence of intratumoral lymphangiogenesis in non-small cell lung cancer. Tumori. 96:734–743. 2010.

18 

Chen H, Xue J, Zhang Y, Zhu X, Gao J and Yu B: Comparison of quantum dots immunofluorescence histochemistry and conventional immunohistochemistry for the detection of caveolin-1 and PCNA in the lung cancer tissue microarray. J Mol Histol. 40:261–268. 2009.

19 

He Y, Zhao X, Gao J, et al: Quantum dots-based immunofluorescent imaging of stromal fibroblasts caveolin-1 and light chain 3B expression and identification of their clinical significance in human gastric cancer. Int J Mol Sci. 13:13764–13780. 2012.

20 

Li ZH, Peng J and Chen HL: Bioconjugated quantum dots as fluorescent probes for biomedical imaging. J Nanosci Nanotechnol. 11:7521–7536. 2011.

21 

Karpathiou G, Sivridis E, Koukourakis MI, et al: Light-chain 3A autophagic activity and prognostic significance in non-small cell lung carcinomas. Chest. 140:127–134. 2011.

22 

Sivridis E, Koukourakis MI, Zois CE, et al: LC3A-positive light microscopy detected patterns of autophagy and prognosis in operable breast carcinomas. Am J Pathol. 176:2477–2489. 2010.

23 

Yoshioka A, Miyata H, Doki Y, et al: LC3, an autophagosome marker, is highly expressed in gastrointestinal cancers. Int J Oncol. 33:461–468. 2008.

24 

Liang XH, Jackson S, Seaman M, et al: Induction of autophagy and inhibition of tumorigenesis by beclin 1. Nature. 402:672–676. 1999.

25 

Jiang ZF, Shao LJ, Wang WM, Yan XB and Liu RY: Decreased expression of Beclin-1 and LC3 in human lung cancer. Mol Biol Rep. 39:259–267. 2012.

26 

Nicotra G, Mercalli F, Peracchio C, et al: Autophagy-active beclin-1 correlates with favourable clinical outcome in non-Hodgkin lymphomas. Mod Pathol. 23:937–950. 2010.

27 

Geng QR, Xu DZ, He LJ, et al: Beclin-1 expression is a significant predictor of survival in patients with lymph node-positive gastric cancer. PLoS One. 7:e459682012.

28 

Zhu W, Pan X, Li F, Zhang Y and Lu X: Expression of Beclin 1 and LC3 in FIGO stage I–II cervical squamous cell carcinoma and relationship to survival. Tumour Biol. 33:1653–1659. 2012.

29 

Fujii S, Mitsunaga S, Yamazaki M, et al: Autophagy is activated in pancreatic cancer cells and correlates with poor patient outcome. Cancer Sci. 99:1813–1819. 2008.

30 

Deretic V and Levine B: Autophagy, immunity, and microbial adaptations. Cell Host Microbe. 5:527–549. 2009.

31 

Jordan TX and Randall G: Manipulation or capitulation: virus interactions with autophagy. Microbes Infect. 14:126–139. 2012.

32 

Griffin LM, Cicchini L and Pyeon D: Human papillomavirus infection is inhibited by host autophagy in primary human keratinocytes. Virology. 437:12–19. 2013.

33 

Ishii Y: Electron microscopic visualization of autophagosomes induced by infection of human papillomavirus pseudovirions. Biochem Biophys Res Commun. 433:385–389. 2013.

34 

Spangle JM and Münger K: The human papillomavirus type 16 E6 oncoprotein activates mTORC1 signaling and increases protein synthesis. J Virol. 84:9398–9407. 2010.

35 

Clarke MA, Wentzensen N, Mirabello L, et al: Human papillomavirus DNA methylation as a potential biomarker for cervical cancer. Cancer Epidemiol Biomarkers Prev. 21:2125–2137. 2012.

36 

Surviladze Z, Sterk RT, DeHaro SA and Ozbun MA: Cellular entry of human papillomavirus type 16 involves activation of the phosphatidylinositol 3-kinase/Akt/mTOR pathway and inhibition of autophagy. J Virol. 87:2508–2517. 2013.

37 

Chen TC, Hung YC, Lin TY, et al: Human papillomavirus infection and expression of ATPase family AAA domain containing 3A, a novel anti-autophagy factor, in uterine cervical cancer. Int J Mol Med. 28:689–696. 2011.

38 

Feng W, Duan X, Liu J, Xiao J and Brown RE: Morphoproteomic evidence of constitutively activated and overexpressed mTOR pathway in cervical squamous carcinoma and high grade squamous intraepithelial lesions. Int J Clin Exp Pathol. 2:249–260. 2009.

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Copy and paste a formatted citation
Spandidos Publications style
Wang HY, Yang GF, Huang YH, Huang QW, Gao J, Zhao XD, Huang LM and Chen HL: Reduced expression of autophagy markers correlates with high‑risk human papillomavirus infection in human cervical squamous cell carcinoma. Oncol Lett 8: 1492-1498, 2014.
APA
Wang, H., Yang, G., Huang, Y., Huang, Q., Gao, J., Zhao, X. ... Chen, H. (2014). Reduced expression of autophagy markers correlates with high‑risk human papillomavirus infection in human cervical squamous cell carcinoma. Oncology Letters, 8, 1492-1498. https://doi.org/10.3892/ol.2014.2417
MLA
Wang, H., Yang, G., Huang, Y., Huang, Q., Gao, J., Zhao, X., Huang, L., Chen, H."Reduced expression of autophagy markers correlates with high‑risk human papillomavirus infection in human cervical squamous cell carcinoma". Oncology Letters 8.4 (2014): 1492-1498.
Chicago
Wang, H., Yang, G., Huang, Y., Huang, Q., Gao, J., Zhao, X., Huang, L., Chen, H."Reduced expression of autophagy markers correlates with high‑risk human papillomavirus infection in human cervical squamous cell carcinoma". Oncology Letters 8, no. 4 (2014): 1492-1498. https://doi.org/10.3892/ol.2014.2417
Copy and paste a formatted citation
x
Spandidos Publications style
Wang HY, Yang GF, Huang YH, Huang QW, Gao J, Zhao XD, Huang LM and Chen HL: Reduced expression of autophagy markers correlates with high‑risk human papillomavirus infection in human cervical squamous cell carcinoma. Oncol Lett 8: 1492-1498, 2014.
APA
Wang, H., Yang, G., Huang, Y., Huang, Q., Gao, J., Zhao, X. ... Chen, H. (2014). Reduced expression of autophagy markers correlates with high‑risk human papillomavirus infection in human cervical squamous cell carcinoma. Oncology Letters, 8, 1492-1498. https://doi.org/10.3892/ol.2014.2417
MLA
Wang, H., Yang, G., Huang, Y., Huang, Q., Gao, J., Zhao, X., Huang, L., Chen, H."Reduced expression of autophagy markers correlates with high‑risk human papillomavirus infection in human cervical squamous cell carcinoma". Oncology Letters 8.4 (2014): 1492-1498.
Chicago
Wang, H., Yang, G., Huang, Y., Huang, Q., Gao, J., Zhao, X., Huang, L., Chen, H."Reduced expression of autophagy markers correlates with high‑risk human papillomavirus infection in human cervical squamous cell carcinoma". Oncology Letters 8, no. 4 (2014): 1492-1498. https://doi.org/10.3892/ol.2014.2417
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