Reduced expression of autophagy markers correlates with high‑risk human papillomavirus infection in human cervical squamous cell carcinoma

Wang,Hua‑Yi; Yang,Gui‑Fang; Huang,Yan‑Hua; Huang,Qi‑Wen; Gao,Jun; Zhao,Xian‑Da; Huang,Li‑Ming; Chen,Hong‑Lei

doi:10.3892/ol.2014.2417

Reduced expression of autophagy markers correlates with high‑risk human papillomavirus infection in human cervical squamous cell carcinoma

Authors:
- Hua‑Yi Wang
- Gui‑Fang Yang
- Yan‑Hua Huang
- Qi‑Wen Huang
- Jun Gao
- Xian‑Da Zhao
- Li‑Ming Huang
- Hong‑Lei Chen
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Affiliations: Department of Pathology, Jingzhou Second People's Hospital, Jingzhou, Hubei 434000, P.R. China, Department of Pathology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China, Department of Molecular Pathology, Wuhan Nano Tumor Diagnosis Engineering Research Center, Wuhan, Hubei 430075, P.R. China, Department of Pathology, School of Basic Medical Science, Wuhan University, P.R. China, Department of Oncology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei 430071, P.R. China
Published online on: August 5, 2014 https://doi.org/10.3892/ol.2014.2417
Pages: 1492-1498

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Abstract

Infection by an oncogenic human papillomavirus (HPV), in particular HPV16 and 18, is a high risk factor for developing cervical cancer; however, viral infection alone is not sufficient for cancer progression. Autophagy is hypothesized to be an important process during carcinogenesis. The aim of the present study was to investigate the association between autophagy and high‑risk HPV (hrHPV) infection in human cervical squamous cell carcinomas (SCCs), and to analyze the clinical significance of this association. Quantum dot (QD)‑based immunofluorescence histochemistry was used to detect the expression of autophagy markers, Beclin‑1 and microtubule‑associated proteins 1A/1B light chain 3B (LC3B) proteins, in 104 cases of cervical cancer (including 80 SCCs and 24 adenocarcinomas) and 20 normal cervical tissues. hrHPV (HPV16/18) infection was detected by QDs based fluorescence in situ hybridization in cervical cancers. The results revealed that the expression levels of Beclin‑1 and LC3B were significantly lower in cervical cancer cells when compared with those of normal cervical squamous epithelial cells, and were found to negatively correlate with hrHPV infection. The expression levels of Beclin‑1 and LC3B were not associated with age, tumor grade, tumor stage, tumor node metastasis stage or lymph node metastasis. However, a positive correlation was identified between Beclin‑1 and LC3B protein expression. In addition, the absence of autophagy in combination with hrHPV infection may accelerate the progression of cervical SCC. In conclusion, decreased expression of Beclin‑1 and LC3B may be important in cervical carcinogenesis. The hrHPV‑host cell interaction may inhibit autophagy, which may aid virus duplication and infection, as well as cervical cancer development.

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