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Article

Interleukin-8 does not influence proliferation of the SGC7901 gastric cancer cell line

  • Authors:
    • Jun Shi
    • Pin-Kang Wei
  • View Affiliations / Copyright

    Affiliations: Department of Traditional Chinese Medicine, Shanghai Changzheng Hospital, The Second Military Medical University, Shanghai 200003, P.R. China
  • Pages: 2475-2480
    |
    Published online on: September 12, 2014
       https://doi.org/10.3892/ol.2014.2531
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Abstract

Interleukin-8 (IL-8), an important inflammatory factor, is induced by Helicobacter pylori infection and is clearly associated with gastric cancer. IL-8 levels have been revealed to correlate significantly with the adhesion, migration and invasion of gastric cancer cells. However, whether IL-8 influences cell proliferation in gastric cancer remains unclear. In the present study, the effect of IL-8 on the proliferation of the SGC7901 human gastric cancer cell line was investigated. SGC7901 cells were exposed to various concentrations of IL-8 (0, 0.2, 0.5, 0.8 and 1 ng/ml) for one to seven days. Cell proliferation was detected by Cell Counting Kit-8 assay. In addition, proliferating cell nuclear antigen (PCNA) protein and mRNA levels were measured by immunofluorescence, western blotting and quantitative polymerase chain reaction. Following exposure of SGC7901 cells to the various concentrations of IL‑8, no significant changes in terms of cell proliferation were identified. However, IL-8 was shown to regulate PCNA protein and mRNA expression levels; at a concentration of 0.8 ng/ml, IL-8 significantly elevated the PCNA protein and mRNA expression levels, whereas IL‑8 significantly inhibited these levels at other concentrations, compared with no treatment. In conclusion, IL-8 does not affect the proliferation of SGC7901 cells. However, IL-8 dosage was associated with PCNA protein and mRNA expression levels.
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1 

Ferro A, Peleteiro B, Malvezzi M, et al: Worldwide trends in gastric cancer mortality (1980–2011), with predictions to 2015, and incidence by subtype. Eur J Cancer. 50:1330–1344. 2014.

2 

Ferlay J, Soerjomataram I, Ervik M, Forman D, Bray F, Dikshit R, Elser S, Mathers C, Rebelo M and Parkin DM: GLOBOCAN 2012 v10, Cancer Incidence and Mortality Worldwide. IARC CancerBase No. 11 Lyon, France: International Agency for Research on Cancer; 2013, Available from: http://globocan.iarc.fr. accessed on August 30, 2014

3 

Harada A, Sekido N, Akahoshi T, et al: Essential involvement of interleukin-8 (IL-8) in acute inflammation. J Leukoc Biol. 56:559–564. 1994.

4 

Raman D, Baugher PJ, Thu YM and Richmond A: Role of chemokines in tumor growth. Cancer Lett. 256:137–165. 2007.

5 

Xie K: Interleukin-8 and human cancer biology. Cytokine Growth Factor Rev. 12:375–391. 2001.

6 

Xu L and Fidler IJ: Interleukin-8: an autocrine growth factor for human ovarian cancer. Oncol Res. 12:97–106. 2000.

7 

Huang S, Mills L, Mian B, et al: Fully humanized neutralizing antibodies to interleukin-8 (ABX-IL8) inhibit angiogenesis, tumor growth, and metastasis of human melanoma. Am J Pathol. 161:125–134. 2002.

8 

Kitadai Y, Haruma K, Mukaida N, et al: Regulation of disease-progression genes in human gastric carcinoma cells by interleukin-8. Clin Cancer Res. 6:2735–2740. 2000.

9 

Kuai WX, Wang Q, Yang XZ, et al: Interleukin-8 associates with adhesion, migration, invasion and chemosensitivity of human gastric cancer cells. World J Gastroenterol. 18:979–985. 2012.

10 

Peek RM Jr and Blaser MJ: Helicobacter pylori and gastrointestinal tract adenocarcinomas. Nat Rev Cancer. 2:28–37. 2002.

11 

Ren Z, Pang G, Clancy R, et al: Shift of the gastric T-cell response in gastric carcinoma. J Gastroenterol Hepatol. 16:142–148. 2001.

12 

Sheh A, Chaturvedi R, Merrell DS, et al: Phylogeographic origin of Helicobacter pylori determines host-adaptive responses upon coculture with gastric epithelial cells. Infect Immun. 81:2468–2477. 2013.

13 

Wang YC and Huang KM: In vitro anti-inflammatory effect of apigenin in the Helicobacter pylori-infected gastric adenocarcinoma cells. Food Chem Toxicol. 53:376–383. 2013.

14 

Yamaoka Y, Kita M, Kodama T, et al: Induction of various cytokines and development of severe mucosal inflammation by cagA gene positive Helicobacter pylori strains. Gut. 41:442–451. 1997.

15 

D’Elios MM and Andersen LP: Helicobacter pylori inflammation, immunity, and vaccines. Helicobacter. 12(Suppl 1): 15–19. 2007.

16 

D’Elios MM and Andersen LP: Inflammation, immunity, and vaccines for Helicobacter pylori. Helicobacter. 14(Suppl 1): 21–28. 2009.

17 

Montecucco C and Rappuoli R: Living dangerously; How Helicobacter pylori survives in the human stomach. Nat Rev Mol Cell Biol. 2:457–466. 2001.

18 

Naito Y and Yoshikawa T: Molecular and cellular mechanisms involved in Helicobacter pylori-induced inflammation and oxidative stress. Free Radic Biol Med. 33:323–336. 2002.

19 

Peek RM Jr, Fiske C and Wilson KT: Role of innate immunity in Helicobacter pylori-induced gastric malignancy. Physiol Rev. 90:831–858. 2010.

20 

Chiba T, Marusawa H, Seno H and Watanabe N: Mechanism for gastric cancer development by Helicobacter pylori infection. J Gastroenterol Hepatol. 23:1175–1181. 2008.

21 

Nozawa Y, Nishihara K, Peek RM, et al: Identification of a signaling cascade for interleukin-8 production by Helicobacter pylori in human gastric epithelial cells. Biochem Pharmacol. 64:21–30. 2002.

22 

Sharma SA, Tummuru MK, Blaser MJ and Kerr LD: Activation of IL-8 gene expression by Helicobacter pylori is regulated by transcription factor nuclear factor-kappa B in gastric epithelial cells. J Immunol. 160:2401–2407. 1998.

23 

Keates S, Keates AC, Warny M, et al: Differential activation of mitogen-activated protein kinases in AGS gastric epithelial cells by cag+ and cag− Helicobacter pylori. J Immunol. 163:5552–5559. 1999.

24 

Eftang LL, Esbensen Y, Tannæs TM, Bukholm IR and Bukholm G: Interleukin-8 is the single most up-regulated gene in whole genome profiling of H. Pylori exposed gastric epithelial cells. BMC Microbiol. 12:92012.

25 

Crabtree JE, Farmery SM, Lindley IJ, et al: CagA/cytotoxic strains of Helicobacter pylori and interleukin-8 in gastric epithelial cell lines. J Clin Pathol. 47:945–950. 1994.

26 

Sharma SA, Tummuru MK, Miller GG and Blaser MJ: Interleukin-8 response of gastric epithelial cell lines to Helicobacter pylori stimulation in vitro. Infect Immun. 63:1681–1687. 1995.

27 

Uemura N, Okamoto S, Yamamoto S, et al: Helicobacter pylori infection and the development of gastric cancer. N Engl J Med. 345:784–789. 2001.

28 

Correa P and Houghton J: Carcinogenesis of Helicobacter pylori. Gastroenterology. 133:659–672. 2007.

29 

World Health Organization; International Agency for Research on Cancer (IARC). IARC monographs on the evaluation of carcinogenic risks to humans. 61. World Health Organization; Geneva, Switzerland: pp. 177–240. 1994

30 

Waugh DJ and Wilson C: The interleukin-8 pathway in cancer. Clin Cancer Res. 14:6735–6741. 2008.

31 

Takagi A, Kamiya S, Koga Y, et al: Analysis of interleukin-8 secretion induced by Helicobacter pylori from the gastric epithelial cell line MKN45: a mechanism independent of the intensity of cytotoxicity. J Gastroenterol Hepatol. 12:368–372. 1997.

32 

Kitadai Y, Haruma K, Sumii K, et al: Expression of interleukin-8 correlates with vascularity in human gastric carcinomas. Am J Pathol. 152:93–100. 1998.

33 

Kido S, Kitadai Y, Hattori N, et al: Interleukin-8 and vascular endothelial growth factor - prognostic factors in human gastric carcinomas? Eur J Cancer. 37:1482–1487. 2001.

34 

Ju D, Sun D, Xiu L, et al: Interleukin-8 is associated with adhesion, migration and invasion in human gastric cancer SCG-7901 cells. Med Oncol. 29:91–99. 2012.

35 

Zhu YM and Woll PJ: Mitogenic effects of interleukin-8/CXCL8 on cancer cells. Future Oncol. 1:699–704. 2005.

36 

Gabellini C, Trisciuoglio D, Desideri M, et al: Functional activity of CXCL8 receptors, CXCR1 and CXCR2, on human malignant melanoma progression. Eur J Cancer. 45:2618–2627. 2009.

37 

Christofakis EP, Miyazaki H, Rubink DS and Yeudall WA: Roles of CXCL8 in squamous cell carcinoma proliferation and migration. Oral Oncol. 44:920–926. 2008.

38 

Wu S, Shang H, Cui L, et al: Targeted blockade of interleukin-8 abrogates its promotion of cervical cancer growth and metastasis. Mol Cell Biochem. 375:69–79. 2013.

39 

Wang Y, Xu RC, Zhang XL, et al: Interleukin-8 secretion by ovarian cancer cells increases anchorage-independent growth, proliferation, angiogenic potential, adhesion and invasion. Cytokine. 59:145–155. 2012.

40 

Luppi F, Longo AM, de Boer WI, Rabe KF and Hiemstra PS: Interleukin-8 stimulates cell proliferation in non-small cell lung cancer through epidermal growth factor receptor transactivation. Lung Cancer. 56:25–33. 2007.

41 

Ning Y, Manegold PC, Hong YK, et al: Interleukin-8 is associated with proliferation, migration, angiogenesis and chemosensitivity in vitro and in vivo in colon cancer cell line models. Int J Cancer. 128:2038–2049. 2011.

42 

Shao N, Chen LH, Ye RY, Lin Y and Wang SM: The depletion of interleukin-8 causes cell cycle arrest and increases the efficacy of docetaxel in breast cancer cells. Biochem Biophys Res Commun. 431:535–541. 2013.

43 

Iwai M, Matsuda M and Iwai Y: Cloning of a cancer cell-producing hepatocyte growth factor, vascular endothelial growth factor, and interleukin-8 from gastric cancer cells. In Vitro Cell Dev Biol Anim. 39:288–290. 2003.

44 

Kubo F, Ueno S, Hiwatashi K, et al: Interleukin-8 in human hepatocellular carcinoma correlates with cancer cell invasion of vessels but not with tumor angiogenesis. Ann Surg Oncol. 12:800–807. 2005.

45 

Lin Y, Wang SM, Lü WM and Huang RP: Effect of interleukin-8 in cell invasion and proliferation of human breast cancer. Zhonghua Wai Ke Za Zhi. 43:1541–1544. 2005.(In Chinese).

46 

Jain S, Filipe MI, Hall PA, et al: Prognostic value of proliferating cell nuclear antigen in gastric carcinoma. J Clin Pathol. 44:655–659. 1991.

47 

Li N: Proliferating cell nuclear antigen (PCNA/cyclin) in gastric carcinoma in relation to its prognosis. Zhonghua Zhong Liu Za Zhi. 15:34–36. 1993.(In Chinese).

48 

Shibata W and Maeda S: Mechanism of H. pylori-induced gastric inflammation and carcinogenesis. Nihon Rinsho. 71:1346–1351. 2013.(In Japanese).

49 

Song H, Michel A, Nyrén O, Ekström AM, Pawlita M and Ye W: A CagA-independent cluster of antigens related to the risk of noncardia gastric cancer: associations between Helicobacter pylori antibodies and gastric adenocarcinoma explored by multiplex serology. Int J Cancer. 134:2942–2950. 2014.

50 

Yoshida T, Kato J, Inoue I, Yoshimura N, Deguchi H, Mukoubayashi C, Oka M, Watanabe M, Enomoto S, Niwa T, et al: Cancer development based on chronic active gastritis and resulting gastric atrophy as assessed by serum levels of pepsinogen and Helicobacter pylori antibody titer. Int J Cancer. 134:1445–1457. 2014.

51 

Berber U, Yılmaz I, Erkul BE and Kaplan M: Peptic ulcer and intestinal metaplasia associated with Helicobacter pylori colonization in gastric heterotopia of the tongue. Turk J Gastroenterol. 25:224–225. 2014.

52 

Kara N, Urganci N, Kalyoncu D and Yilmaz B: The association between Helicobacter pylori gastritis and lymphoid aggregates, lymphoid follicles and intestinal metaplasia in gastric mucosa of children. J Paediatr Child Health. 50:605–609. 2014.

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Copy and paste a formatted citation
Spandidos Publications style
Shi J and Wei P: Interleukin-8 does not influence proliferation of the SGC7901 gastric cancer cell line. Oncol Lett 8: 2475-2480, 2014.
APA
Shi, J., & Wei, P. (2014). Interleukin-8 does not influence proliferation of the SGC7901 gastric cancer cell line. Oncology Letters, 8, 2475-2480. https://doi.org/10.3892/ol.2014.2531
MLA
Shi, J., Wei, P."Interleukin-8 does not influence proliferation of the SGC7901 gastric cancer cell line". Oncology Letters 8.6 (2014): 2475-2480.
Chicago
Shi, J., Wei, P."Interleukin-8 does not influence proliferation of the SGC7901 gastric cancer cell line". Oncology Letters 8, no. 6 (2014): 2475-2480. https://doi.org/10.3892/ol.2014.2531
Copy and paste a formatted citation
x
Spandidos Publications style
Shi J and Wei P: Interleukin-8 does not influence proliferation of the SGC7901 gastric cancer cell line. Oncol Lett 8: 2475-2480, 2014.
APA
Shi, J., & Wei, P. (2014). Interleukin-8 does not influence proliferation of the SGC7901 gastric cancer cell line. Oncology Letters, 8, 2475-2480. https://doi.org/10.3892/ol.2014.2531
MLA
Shi, J., Wei, P."Interleukin-8 does not influence proliferation of the SGC7901 gastric cancer cell line". Oncology Letters 8.6 (2014): 2475-2480.
Chicago
Shi, J., Wei, P."Interleukin-8 does not influence proliferation of the SGC7901 gastric cancer cell line". Oncology Letters 8, no. 6 (2014): 2475-2480. https://doi.org/10.3892/ol.2014.2531
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