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December-2014 Volume 8 Issue 6

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Article

Distinct allelic expression patterns of imprinted IGF2 in adenocarcinoma and squamous cell carcinoma of the lung

  • Authors:
    • Satoru Ozaki
    • Ei Kawahara
    • Shiori Maenaka
    • Nguyen Viet Hoang
    • Takeru Oyama
    • Miwa Imai
    • Makoto Oda
    • Seiji Yano
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory Science, Kanazawa University, Ishikawa 920‑0942, Japan, Department of Pathology, Kanazawa University, Ishikawa 920‑0942, Japan, Department of Health Science, Ishikawa Prefectural Nursing University, Ishikawa 929‑1212, Japan, Department of Lung Surgery, Kanazawa University Hospital, Ishikawa 920‑8641, Japan, Department of Internal Medicine, Kanazawa University Cancer Research Institute, Ishikawa 920-1192, Japan
  • Pages: 2561-2564
    |
    Published online on: September 29, 2014
       https://doi.org/10.3892/ol.2014.2572
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Abstract

The insulin-like growth factor 2 gene (IGF2) is an imprinting gene, which mediates cell growth and apoptosis. The loss of imprinting (LOI) of IGF2 has been associated with the development of cancer. In the present study, loss LOI of IGF2 in lung cancer was analyzed using polymerase chain reaction‑restriction fragment length polymorphism (PCR‑RFLP) in combination with DNA sequencing of samples collected by laser capture microdissection. The status of each sample was assigned as imprinting when PCR‑RFLP revealed only one band or sequence with a single peak; otherwise, the case was classified as LOI. LOI was identified in eight out of 13 adenocarcinoma cases (62%), but was not detected in any of the nine squamous cell carcinoma cases (0%). These results suggest that IGF2 LOI is involved in the molecular pathogenesis of lung adenocarcinoma, but not squamous cell carcinoma, and that LOI may be detected through increased IGF2 expression levels.
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Copy and paste a formatted citation
Spandidos Publications style
Ozaki S, Kawahara E, Maenaka S, Hoang NV, Oyama T, Imai M, Oda M and Yano S: Distinct allelic expression patterns of imprinted IGF2 in adenocarcinoma and squamous cell carcinoma of the lung. Oncol Lett 8: 2561-2564, 2014.
APA
Ozaki, S., Kawahara, E., Maenaka, S., Hoang, N.V., Oyama, T., Imai, M. ... Yano, S. (2014). Distinct allelic expression patterns of imprinted IGF2 in adenocarcinoma and squamous cell carcinoma of the lung. Oncology Letters, 8, 2561-2564. https://doi.org/10.3892/ol.2014.2572
MLA
Ozaki, S., Kawahara, E., Maenaka, S., Hoang, N. V., Oyama, T., Imai, M., Oda, M., Yano, S."Distinct allelic expression patterns of imprinted IGF2 in adenocarcinoma and squamous cell carcinoma of the lung". Oncology Letters 8.6 (2014): 2561-2564.
Chicago
Ozaki, S., Kawahara, E., Maenaka, S., Hoang, N. V., Oyama, T., Imai, M., Oda, M., Yano, S."Distinct allelic expression patterns of imprinted IGF2 in adenocarcinoma and squamous cell carcinoma of the lung". Oncology Letters 8, no. 6 (2014): 2561-2564. https://doi.org/10.3892/ol.2014.2572
Copy and paste a formatted citation
x
Spandidos Publications style
Ozaki S, Kawahara E, Maenaka S, Hoang NV, Oyama T, Imai M, Oda M and Yano S: Distinct allelic expression patterns of imprinted IGF2 in adenocarcinoma and squamous cell carcinoma of the lung. Oncol Lett 8: 2561-2564, 2014.
APA
Ozaki, S., Kawahara, E., Maenaka, S., Hoang, N.V., Oyama, T., Imai, M. ... Yano, S. (2014). Distinct allelic expression patterns of imprinted IGF2 in adenocarcinoma and squamous cell carcinoma of the lung. Oncology Letters, 8, 2561-2564. https://doi.org/10.3892/ol.2014.2572
MLA
Ozaki, S., Kawahara, E., Maenaka, S., Hoang, N. V., Oyama, T., Imai, M., Oda, M., Yano, S."Distinct allelic expression patterns of imprinted IGF2 in adenocarcinoma and squamous cell carcinoma of the lung". Oncology Letters 8.6 (2014): 2561-2564.
Chicago
Ozaki, S., Kawahara, E., Maenaka, S., Hoang, N. V., Oyama, T., Imai, M., Oda, M., Yano, S."Distinct allelic expression patterns of imprinted IGF2 in adenocarcinoma and squamous cell carcinoma of the lung". Oncology Letters 8, no. 6 (2014): 2561-2564. https://doi.org/10.3892/ol.2014.2572
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