Polyamine analog TBP inhibits proliferation of human K562 chronic myelogenous leukemia cells by induced apoptosis

Wang,Qing; Wang,Yan‑Lin; Wang,Kai; Yang,Jian‑Lin; Cao,Chun‑Yu

doi:10.3892/ol.2014.2615

January-2015 Volume 9 Issue 1

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January-2015 Volume 9 Issue 1

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Article

Polyamine analog TBP inhibits proliferation of human K562 chronic myelogenous leukemia cells by induced apoptosis

Authors:
- Qing Wang
- Yan‑Lin Wang
- Kai Wang
- Jian‑Lin Yang
- Chun‑Yu Cao
View Affiliations / Copyright

Affiliations: Institute of Molecular Biology, Medical College, China Three Gorges University, Yichang, Hubei 443002, P.R. China, College of Chemical and Pharmacy, Wuhan Engineering University, Wuhan, Hubei 430073, P.R. China
Pages: 278-282
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Published online on: October 13, 2014

https://doi.org/10.3892/ol.2014.2615
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Abstract

The aim of the present study was to investigate the effects of the novel polyamine analog tetrabutyl propanediamine (TBP) on the growth of K562 chronic myelogenous leukemia (CML) cells and the underlying mechanism of these effects. MTT was used for the analysis of cell proliferation and flow cytometry was performed to analyze cell cycle distribution. DNA fragmentation analysis and Annexin V/propidium iodide double staining were used to identify apoptotic cells. The activity of the key enzymes in polyamine catabolism was detected using chemiluminescence. TBP can induce apoptosis and significantly inhibit K562 cell proliferation in a time‑ and dose‑dependent manner. TBP treatment significantly induced the enzyme activity of spermine oxidase and acetylpolyamine oxidase in K562 cells, and also enhanced the inhibitory effect of the antitumor drug doxorubicin on K562 cell proliferation. As a novel polyamine analog, TBP significantly inhibited proliferation and induced apoptosis in K562 cells by upregulating the activity of the key enzymes in the polyamine catabolic pathways. TBP also increased the sensitivity of the K562 cells to the antitumor drug doxorubicin. These data indicate an important potential value of TBP for clinical therapy of human CML.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Polyamine analog TBP inhibits proliferation of human K562 chronic myelogenous leukemia cells by induced apoptosis

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Abstract

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