Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
January-2015 Volume 9 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
January-2015 Volume 9 Issue 1

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

P2X7 mRNA expression in non‑small cell lung cancer: MicroRNA regulation and prognostic value

  • Authors:
    • Laura Boldrini
    • Mirella Giordano
    • Greta Alì
    • Franca Melfi
    • Gaetano Romano
    • Marco Lucchi
    • Gabriella Fontanini
  • View Affiliations / Copyright

    Affiliations: Department of Surgical, Medical, Molecular Pathology and Critical Area, University of Pisa, Pisa 56126, Italy, Unit of Pathological Anatomy III, University Hospital of Pisa, Pisa 56126, Italy, Unit of Thoracic Surgery, University Hospital of Pisa, Pisa 56126, Italy
  • Pages: 449-453
    |
    Published online on: October 15, 2014
       https://doi.org/10.3892/ol.2014.2620
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The human P2X7 receptor is significant and exhibits several functions in neoplasia. At present, little is known with regard to its regulation. P2X7 expression may be regulated post‑transcriptionally and putative microRNA (miRNA) binding sites are considered to be involved. The aim of this study was to determine whether miRNAs (miR‑21, let‑7 g and miR‑205) regulate P2X7 mRNA stability. In addition, the impact of P2X7 expression in patients with non‑small cell lung cancer (NSCLC) was investigated. P2X7 mRNA and mature Let‑7 g, miR‑21, and miR‑205 expression levels were quantified in 96 NSCLC cases using quantitative reverse transcription polymerase chain reaction. In all samples, epidermal growth factor receptor and K‑Ras mutational analysis was also performed. Samples with low P2X7 expression were found to exhibit a higher fold change in miR‑21 expression when compared with samples exhibiting high P2X7 expression. Significantly higher miR‑21 expression was observed in the tumors of NSCLC patients with a K‑Ras mutation when compared with patients who had K‑Ras wild‑type tumors (P=0.003). Additionally, to evaluate the association between P2X7 expression and prognosis in NSCLC patients, survival analysis was performed using the Kaplan‑Meier method. A significant difference in the progression‑free survival and overall survival in the NSCLC patients with high P2X7 expression was identified, when compared with that of patients with low expression (P=0.03 and P=0.02, respetively). Therefore, we hypothesized that high levels of miR‑21 expression in NSCLC patients with K‑Ras mutations may be regulated by a complex circuit, including P2X7 downregulation and together these processes may promote tumor progression.
View Figures

Figure 1

View References

1 

Buell GN, Talabot F, Gos A, Lorenz J, Lai E, Morris MA and Antonarakis SE: Gene structure and chromosomal localization of the human P2X7 receptor. Receptors Channels. 5:347–354. 1998.PubMed/NCBI

2 

Sluyter R and Stokes L: Significance of P2X7 receptor variants to human health and disease. Recent Pat DNA Gene Seq. 5:41–54. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Li X, Qi X, Zhou L, Catera D, Rote NS, Potashkin J, Abdul-Karim FW and Gorodeski GI: Decreased expression of P2X7 in endometrial epithelial pre-cancerous and cancer cells. Gynecol Oncol. 106:233–243. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Zhou L, Qi X, Potashkin JA, Abdul-Karim FW and Gorodeski GI: MicroRNAs miR-186 and miR-150 down-regulate expression of the pro-apoptotic purinergic P2X7 receptor by activation of instability sites at the 3′-untranslated region of the gene that decrease steadystate levels of the transcript. J Biol Chem. 283:28274–28286. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Rahman OA, Sasvari-Szekely M, Szekely A, Faludi G, Guttman A and Nemoda Z: Analysis of a polymorphic microRNA target site in the purinergic receptor P2RX7 gene. Electrophoresis. 31:1790–1795. 2010. View Article : Google Scholar : PubMed/NCBI

6 

Lu J, Getz G, Miska EA, et al: MicroRNA expression profiles classify human cancers. Nature. 435:834–838. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Bagga S, Bracht J, Hunter S, Massirer K, Holtz J, Eachus R and Pasquinelli AE: Regulation by let-7 and lin-4 miRNAs results in target mRNA degradation. Cell. 122:553–563. 2005. View Article : Google Scholar : PubMed/NCBI

8 

Lim LP, Lau NC, Garrett-Engele P, et al: Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature. 433:769–773. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Lai EC: Predicting and validating microRNA targets. Genome Biol. 5:1152004. View Article : Google Scholar : PubMed/NCBI

10 

Lewis BP, Burge CB and Bartel DP: Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 120:15–20. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Xie X, Lu J, Kulbokas EJ, et al: Systematic discovery of regulatory motifs in human promoters and 3′ UTRs by comparison of several mammals. Nature. 434:338–345. 2005. View Article : Google Scholar : PubMed/NCBI

12 

Travis WD, Brambilla E, Muller-Hemerlink HK and Harris CC: World Health Organization Classification of Tumors. Pathology and Genetics of Tumors of the Lung, Pleura, Thymus and Heart Lyon, France: IARC Press; 2004

13 

Travis WD, Brambilla E, Noguchi M, et al: International association for the study of lung cancer/American thoracic society/European respiratory society international multidisciplinary classification of lung adenocarcinoma. J Thorac Oncol. 6:244–285. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Capodanno A, Boldrini L, Alì G, Pelliccioni S, Mussi A and Fontanini G: Phosphatidylinositol-3-kinase α catalytic subunit gene somatic mutations in bronchopulmonary neuroendocrine tumors. Oncol Rep. 28:1559–1566. 2012.PubMed/NCBI

15 

Fuller SJ, Stokes L, SkarRatt KK, Gu BJ and Wiley JS: Genetics of the P2X7 receptor and human disease. Purinergic Signal. 5:257–262. 2009. View Article : Google Scholar : PubMed/NCBI

16 

Brennecke J, Stark A, Russell RB and Cohen SM: Principles of microRNA-target recognition. PLoS Biol. 3:e852005. View Article : Google Scholar : PubMed/NCBI

17 

Mulrane L, McGee SF, Gallagher WM and O’Connor DP: miRNA dysregulation in breast cancer. Cancer Res. 73:6554–6562. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Capodanno A, Boldrini L, Proietti A, et al: Let-7g and miR-21 expression in non-small cell lung cancer: correlation with clinicopathological and molecular features. Int J Oncol. 43:765–774. 2013.PubMed/NCBI

19 

Adinolfi E, Raffaghello L, Giuliani AL, et al: Expression of P2X7 receptor increases in vivo tumor growth. Cancer Res. 72:2957–2969. 2012. View Article : Google Scholar : PubMed/NCBI

20 

White N and Burnstock G: P2 receptors and cancer. Trends Pharmacol Sci. 27:211–217. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Adinolfi E, Melchiorri L, Falzoni S, et al: P2X7 receptor expression in evolutive and indolent forms of chronic B lymphocytic leukemia. Blood. 99:706–708. 2002. View Article : Google Scholar : PubMed/NCBI

22 

Chong JH, Zheng GG, Zhu XF, et al: Abnormal expression of P2X family receptors in Chinese pediatric acute leukemias. Biochem Biophys Res Commun. 391:498–504. 2010. View Article : Google Scholar

23 

Raffaghello L, Chiozzi P, Falzoni S, Di Virgilio F and Pistoia V: The P2X7 receptor sustains the growth of human neuroblastoma cells through a substance P-dependent mechanism. Cancer Res. 66:907–914. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Solini A, Cuccato S, Ferrari D, et al: Increased P2X7 receptor expression and function in thyroid papillary cancer: a new potential marker of the disease? Endocrinology. 149:389–396. 2008. View Article : Google Scholar

25 

Slater M, Danieletto S, Pooley M, Cheng TL, Gidley-Baird A and Barden JA: Differentiation between cancerous and normal hyperplastic lobules in breast lesions. Breast Cancer Res Treat. 83:1–10. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Souza CO, Santoro GF, Figliuolo VR, et al: Extracellular ATP induces cell death in human intestinal epithelial cells. Biochim Biophys Acta. 1820:1867–1878. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Bian S, Sun X, Bai A, et al: P2X7 integrates PI3K/AKT and AMPK-PRAS40-mTOR signaling pathways to mediate tumor cell death. PLoS One. 8:e601842013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Boldrini L, Giordano M, Alì G, Melfi F, Romano G, Lucchi M and Fontanini G: P2X7 mRNA expression in non‑small cell lung cancer: MicroRNA regulation and prognostic value. Oncol Lett 9: 449-453, 2015.
APA
Boldrini, L., Giordano, M., Alì, G., Melfi, F., Romano, G., Lucchi, M., & Fontanini, G. (2015). P2X7 mRNA expression in non‑small cell lung cancer: MicroRNA regulation and prognostic value. Oncology Letters, 9, 449-453. https://doi.org/10.3892/ol.2014.2620
MLA
Boldrini, L., Giordano, M., Alì, G., Melfi, F., Romano, G., Lucchi, M., Fontanini, G."P2X7 mRNA expression in non‑small cell lung cancer: MicroRNA regulation and prognostic value". Oncology Letters 9.1 (2015): 449-453.
Chicago
Boldrini, L., Giordano, M., Alì, G., Melfi, F., Romano, G., Lucchi, M., Fontanini, G."P2X7 mRNA expression in non‑small cell lung cancer: MicroRNA regulation and prognostic value". Oncology Letters 9, no. 1 (2015): 449-453. https://doi.org/10.3892/ol.2014.2620
Copy and paste a formatted citation
x
Spandidos Publications style
Boldrini L, Giordano M, Alì G, Melfi F, Romano G, Lucchi M and Fontanini G: P2X7 mRNA expression in non‑small cell lung cancer: MicroRNA regulation and prognostic value. Oncol Lett 9: 449-453, 2015.
APA
Boldrini, L., Giordano, M., Alì, G., Melfi, F., Romano, G., Lucchi, M., & Fontanini, G. (2015). P2X7 mRNA expression in non‑small cell lung cancer: MicroRNA regulation and prognostic value. Oncology Letters, 9, 449-453. https://doi.org/10.3892/ol.2014.2620
MLA
Boldrini, L., Giordano, M., Alì, G., Melfi, F., Romano, G., Lucchi, M., Fontanini, G."P2X7 mRNA expression in non‑small cell lung cancer: MicroRNA regulation and prognostic value". Oncology Letters 9.1 (2015): 449-453.
Chicago
Boldrini, L., Giordano, M., Alì, G., Melfi, F., Romano, G., Lucchi, M., Fontanini, G."P2X7 mRNA expression in non‑small cell lung cancer: MicroRNA regulation and prognostic value". Oncology Letters 9, no. 1 (2015): 449-453. https://doi.org/10.3892/ol.2014.2620
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team