E6-associated transcription patterns in human papilloma virus 16-positive cervical tissues

Lin,Kezhi; Lu,Xulian; Chen,Jun; Zou,Ruanmin; Zhang,Lifang; Xue,Xiangyang

doi:10.3892/ol.2014.2698

January-2015 Volume 9 Issue 1

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January-2015 Volume 9 Issue 1

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Article

E6-associated transcription patterns in human papilloma virus 16-positive cervical tissues

Authors:
- Kezhi Lin
- Xulian Lu
- Jun Chen
- Ruanmin Zou
- Lifang Zhang
- Xiangyang Xue
View Affiliations / Copyright

Affiliations: Medical Experimental Teaching Center, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, Department of Microbiology and Immunology, Institute of Molecular Virology and Immunology, Institute of Tropical Medicine, Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China, Department of Pathology, Zhuji People's Hospital of Zhejiang Province, Zhuji, Zhejiang 311800, P.R. China
Pages: 478-482
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Published online on: November 11, 2014

https://doi.org/10.3892/ol.2014.2698
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Abstract

The change in transcription pattern induced by post‑transcriptional RNA splicing is an important mechanism in the regulation of the early gene expression of human papilloma virus (HPV). The present study was conducted to establish a method to specifically amplify HPV‑16 E6‑associated transcripts. The E6‑related transcripts from 63 HPV‑16‑positive cervical tumor tissue samples were amplified, consisting of eight cases of low‑risk intraepithelial lesions, 38 cases of high‑risk intraepithelial lesions and 17 cases of cervical cancer (CxCa). The appropriate amplified segments were recovered following agarose gel electrophoresis, and subjected to further sequencing and sequence alignment analysis. Six groups of E6 transcription patterns were identified from HPV‑16‑positive cervical tumor tissue, including five newly‑discovered transcripts. Different HPV‑16 E6‑associated transcription patterns were detected during the development of CxCa. Over the course of the progression of the low‑grade squamous intraepithelial lesions to CxCa, the specific HPV‑16 E6‑associated transcription patterns and the dominant transcripts were all different. As indicated by this study, the transcription pattern of the E6 early gene of HPV‑16 was closely associated with the stages of cervical carcinogenesis, and may also be involved in the development of CxCa.

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1	zur Hausen H: Human papillomaviruses in the pathogenesis of anogenital cancer. Virology. 184:9–13. 1991. View Article : Google Scholar : PubMed/NCBI
2	Frisch M, Fenger C, van den Brule AJ, et al: Variants of squamous cell carcinoma of the anal canal and perianal skin and their relation to human papillomaviruses. Cancer Res. 59:753–757. 1999.PubMed/NCBI
3	Castellsagué X, Díaz M, de Sanjosé S, et al; International Agency for Research on Cancer Multicenter Cervical Cancer Study Group. Worldwide human papillomavirus etiology of cervical adenocarcinoma and its cofactors: implications for screening and prevention. J Natl Cancer Inst. 98:303–315. 2006. View Article : Google Scholar : PubMed/NCBI
4	Scheffner M, Werness BA, Huibregtse JM, Levine AJ and Howley PM: The E6 oncoprotein encoded by human papillomavirus types 16 and 18 promotes the degradation of p53. Cell. 63:1129–1136. 1990. View Article : Google Scholar : PubMed/NCBI
5	Wang J, Sampath A, Raychaudhuri P and Bagchi S: Both Rb and E7 are regulated by the ubiquitin proteasome pathway in HPV-containing cervical tumor cells. Oncogene. 20:4740–4749. 2001. View Article : Google Scholar : PubMed/NCBI
6	Incassati A, Patel D and McCance DJ: Induction of tetraploidy through loss of p53 and upregulation of Plk1 by human papillomavirus type-16 E6. Oncogene. 25:2444–2451. 2006. View Article : Google Scholar
7	Zheng ZM and Baker CC: Papillomavirus genome structure, expression, and post-transcriptional regulation. Front Biosci. 11:2286–2302. 2006. View Article : Google Scholar : PubMed/NCBI
8	Schwartz S: HPV-16 RNA processing. Front Biosci. 13:5880–5891. 2008. View Article : Google Scholar : PubMed/NCBI
9	Klaes R, Woerner SM, Ridder R, et al: Detection of high-risk cervical intraepithelial neoplasia and cervical cancer by amplification of transcripts derived from integrated papillomavirus oncogenes. Cancer Res. 59:6132–6136. 1999.
10	Frohman MA, Dush MK and Martin GR: Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA. 85:8998–9002. 1988. View Article : Google Scholar : PubMed/NCBI
11	Wentzensen N, Ridder R, Klaes R, Vinokurova S, Schaefer U and Doeberitz Mv: Characterization of viral-cellular fusion transcripts in a large series of HPV16 and 18 positive anogenital lesions. Oncogene. 21:419–426. 2002. View Article : Google Scholar : PubMed/NCBI
12	Smotkin D, Prokoph H and Wettstein FO: Oncogenic and nononcogenic human genital papillomaviruses generate the E7 mRNA by different mechanisms. J Virol. 63:1441–1447. 1989.PubMed/NCBI
13	Sedman SA, Barbosa MS, Vass WC, et al: The full-length E6 protein of human papillomavirus type 16 has transforming and trans-activating activities and cooperates with E7 to immortalize keratinocytes in culture. J Virol. 65:4860–4866. 1991.PubMed/NCBI
14	Kraus I, Driesch C, Vinokurova S, et al: The majority of viral-cellular fusion transcripts in cervical carcinomas cotranscribe cellular sequences of known or predicted genes. Cancer Res. 68:2514–2522. 2008. View Article : Google Scholar : PubMed/NCBI
15	Schmitt M, Dalstein V, Waterboer T, Clavel C, Gissmann L and Pawlita M: Diagnosing cervical cancer and high-grade precursors by HPV16 transcription patterns. Cancer Res. 70:249–256. 2010. View Article : Google Scholar
16	Schmitz M, Driesch C, Jansen L, Runnebaum IB and Dürst M: Non-random integration of the HPV genome in cervical cancer. PLoS One. 7:e396322012. View Article : Google Scholar : PubMed/NCBI
17	Cornelissen MT, Smits HL, Briët MA, et al: Uniformity of the splicing pattern of the E6/E7 transcripts in human papillomavirus type 16-transformed human fibroblasts, human cervical premalignant lesions and carcinomas. J Gen Virol. 71:1243–1246. 1990. View Article : Google Scholar : PubMed/NCBI
18	Van Tine BA, Kappes JC, Banerjee NS, et al: Clonal selection for transcriptionally active viral oncogenes during progression to cancer. J Virol. 78:11172–11186. 2004. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

E6-associated transcription patterns in human papilloma virus 16-positive cervical tissues

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