search button

MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma

  • Authors:
    • Yuzhi Jiang
    • Yuting Duan
    • Haibin Zhou

  • View Affiliations

  • Published online on: November 11, 2014     https://doi.org/10.3892/ol.2014.2701
  • Pages: 471-477
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

MicroRNAs (miRNAs) are a type of small non‑coding RNA that negatively regulate gene expression levels by binding to the 3'‑untranslated region of specific target mRNAs. To investigate the role of miR‑27a in esophageal squamous cell carcinoma (ESCC), TargetScan software was used to predict the target gene of miR‑27a. Kirsten rat sarcoma viral oncogene homolog (KRAS), which has been implicated as a regulator of cell proliferation, differentiation and transformation, was identified as a potential target gene of miR‑27a and, thus, was the focus of the present study. Luciferase activity in cells transfected with miR‑27a mimics was 48% lower when compared with that of the miRNA‑negative control. Furthermore, expression levels of the K‑ras protein were reduced by ≥50% in cells cotransfected with an expression vector containing miR‑27a and miR‑27a binding sequences, when compared with the control. The expression level of miR‑27a was significantly lower in ESCC cell lines and tissues when compared with healthy esophageal epithelial cells and tissues. However, the expression level of the target gene, KRAS was upregulated and ESCC cell proliferation was significantly inhibited following miR‑27a mimic or small interfering K‑ras transfection. In conclusion, the present study demonstrated that the expression level of miR‑27a was low in ESCC and that miR‑27a directly targets the KRAS gene, resulting in inhibited cell proliferation in esophageal cancer.
View Figures
View References

Related Articles

Journal Cover

January-2015
Volume 9 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 2.9
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 6.3
  • CiteScore Rank: Q2: #119/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.725
  • SCImago Journal Rank (SJR): 0.639
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
Jiang Y, Duan Y and Zhou H: MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma. Oncol Lett 9: 471-477, 2015
APA
Jiang, Y., Duan, Y., & Zhou, H. (2015). MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma. Oncology Letters, 9, 471-477. https://doi.org/10.3892/ol.2014.2701
MLA
Jiang, Y., Duan, Y., Zhou, H."MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma". Oncology Letters 9.1 (2015): 471-477.
Chicago
Jiang, Y., Duan, Y., Zhou, H."MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma". Oncology Letters 9, no. 1 (2015): 471-477. https://doi.org/10.3892/ol.2014.2701