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Article

MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma

  • Authors:
    • Yuzhi Jiang
    • Yuting Duan
    • Haibin Zhou
  • View Affiliations / Copyright

    Affiliations: Department of Radiology, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China, Department of Thoracic Surgery, Liaocheng People's Hospital, Liaocheng, Shandong 252000, P.R. China
  • Pages: 471-477
    |
    Published online on: November 11, 2014
       https://doi.org/10.3892/ol.2014.2701
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Abstract

MicroRNAs (miRNAs) are a type of small non‑coding RNA that negatively regulate gene expression levels by binding to the 3'‑untranslated region of specific target mRNAs. To investigate the role of miR‑27a in esophageal squamous cell carcinoma (ESCC), TargetScan software was used to predict the target gene of miR‑27a. Kirsten rat sarcoma viral oncogene homolog (KRAS), which has been implicated as a regulator of cell proliferation, differentiation and transformation, was identified as a potential target gene of miR‑27a and, thus, was the focus of the present study. Luciferase activity in cells transfected with miR‑27a mimics was 48% lower when compared with that of the miRNA‑negative control. Furthermore, expression levels of the K‑ras protein were reduced by ≥50% in cells cotransfected with an expression vector containing miR‑27a and miR‑27a binding sequences, when compared with the control. The expression level of miR‑27a was significantly lower in ESCC cell lines and tissues when compared with healthy esophageal epithelial cells and tissues. However, the expression level of the target gene, KRAS was upregulated and ESCC cell proliferation was significantly inhibited following miR‑27a mimic or small interfering K‑ras transfection. In conclusion, the present study demonstrated that the expression level of miR‑27a was low in ESCC and that miR‑27a directly targets the KRAS gene, resulting in inhibited cell proliferation in esophageal cancer.
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1 

Lin SW, Abnet CC, Freedman ND, et al: Measuring telomere length for the early detection of precursor lesions of esophageal squamous cell carcinoma. BMC Cancer. 13:5782013. View Article : Google Scholar : PubMed/NCBI

2 

Wu C, Li M, Hu C and Duan H: Prognostic role of microRNA polymorphisms in patients with advanced esophageal squamous cell carcinoma receiving platinum-based chemotherapy. Cancer Chemother Pharmacol. 73:335–341. 2014. View Article : Google Scholar

3 

Ku GY and Ilson DH: Adjuvant (postoperative) therapy for esophageal cancer. Thorac Surg Clin. 23:525–533. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Liu L, Zuo LF and Guo JW: Reversal of multidrug resistance by the anti-malaria drug artesunate in the esophageal cancer Eca109/ABCG2 cell line. Oncol Lett. 6:1475–1481. 2013.PubMed/NCBI

5 

Liu R, Yang M, Meng Y, et al: Tumor-suppressive function of miR-139–5p in esophageal squamous cell carcinoma. PLoS One. 8:e770682013. View Article : Google Scholar

6 

Marks J, Rice DC and Swisher SG: Salvage esophagectomy in the management of recurrent or persistent esophageal carcinoma. Thorac Surg Clin. 23:559–567. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Migita K, Sho M, Shimada K, et al: Significant involvement of herpesvirus entry mediator in human esophageal squamous cell carcinoma. Cancer. 120:808–817. 2014. View Article : Google Scholar

8 

Moghbeli M, Forghanifard MM, Aarabi A, Mansourian A and Abbaszadegan MR: Clinicopathological sex-related relevance of Musashi1 mRNA expression in esophageal squamous cell carcinoma patients. Pathol Oncol Res. Oct 28–2013.(Epub ahead of print).

9 

Nurkin SJ, Nava HR, Yendamuri S, et al: Outcomes of endoscopic resection for high-grade dysplasia and esophageal cancer. Surg Endosc. 28:1090–1095. 2014. View Article : Google Scholar

10 

Oze I, Matsuo K, Kawakita D, et al: Coffee and green tea consumption is associated with upper aerodigestive tract cancer in Japan. Int J Cancer. 135:391–400. 2014. View Article : Google Scholar

11 

Paul S and Altorki N: Induction therapy for esophageal cancer. Thorac Surg Clin. 23:499–507. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Murata K, Ito H, Yoshitomi H, et al: Inhibition of miR-92a enhances fracture healing via promoting angiogenesis in a model of stabilized fracture in young mice. J Bone Miner Res. 29:316–326. 2014. View Article : Google Scholar

13 

Menigatti M, Staiano T, Manser CN, et al: Epigenetic silencing of monoallelically methylated miRNA loci in precancerous colorectal lesions. Oncogenesis. 2:e562013. View Article : Google Scholar : PubMed/NCBI

14 

Guo L, Zhao Y, Zhang H, Yang S and Chen F: Close association between paralogous multiple isomiRs and paralogous/orthologues miRNA sequences implicates dominant sequence selection across various animal species. Gene. 527:624–629. 2013. View Article : Google Scholar : PubMed/NCBI

15 

Chen Y, Min L, Zhang X, et al: Decreased miRNA-148a is associated with lymph node metastasis and poor clinical outcomes and functions as a suppressor of tumor metastasis in non-small cell lung cancer. Oncol Rep. 30:1832–1840. 2013.PubMed/NCBI

16 

Edelstein LC, McKenzie SE, Shaw C, et al: MicroRNAs in platelet production and activation. J Thromb Haemost. 11(Suppl 1): 340–350. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Gu J, Hu W, Wu J, et al: miRNA genes of an invasive vector mosquito, Aedes albopictus. PLoS One. 8:e676382013. View Article : Google Scholar : PubMed/NCBI

18 

Anwar SL, Albat C, Krech T, et al: Concordant hypermethylation of intergenic microRNA genes in human hepatocellular carcinoma as new diagnostic and prognostic marker. Int J Cancer. 133:660–670. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Biscontin A, Casara S, Cagnin S, et al: New miRNA labeling method for bead-based quantification. BMC Mol Biol. 11:442010. View Article : Google Scholar : PubMed/NCBI

20 

Chen X, Huang Z, Chen D, Yang T and Liu G: MicroRNA-27a is induced by leucine and contributes to leucine-induced proliferation promotion in C2C12 cells. Int J Mol Sci. 14:14076–14084. 2013. View Article : Google Scholar : PubMed/NCBI

21 

No authors listed. Update of the NCCN guidelines for treatment of breast cancer. Oncology (Williston Park). 11:199–220. 1997.

22 

Zhang J, Lu Y, Yue X, et al: MiR-124 suppresses growth of human colorectal cancer by inhibiting STAT3. PLoS One. 8:e703002013. View Article : Google Scholar : PubMed/NCBI

23 

Riedl CC, Slobod E, Jochelson M, et al: Retrospective Analysis of 18F-FDG PET/CT for Staging Asymptomatic Breast Cancer Patients Younger Than 40 Years. J Nucl Med. 55:1578–1583. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Kaspi H, Chapnik E, Levy M, et al: Brief report: miR-290–295 regulate embryonic stem cell differentiation propensities by repressing Pax6. Stem Cells. 31:2266–2272. 2013. View Article : Google Scholar : PubMed/NCBI

25 

Liao WT, Li TT, Wang ZG, et al: microRNA-224 promotes cell proliferation and tumor growth in human colorectal cancer by repressing PHLPP1 and PHLPP2. Clin Cancer Res. 19:4662–4672. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Lu J and Bushel PR: Dynamic expression of 3′ UTRs revealed by Poisson hidden Markov modeling of RNA-Seq: implications in gene expression profiling. Gene. 527:616–623. 2013. View Article : Google Scholar : PubMed/NCBI

27 

Pincini A, Tornillo G, Orso F, et al: Identification of p130Cas/ErbB2-dependent invasive signatures in transformed mammary epithelial cells. Cell Cycle. 12:2409–2422. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Xu Q, He CY, Liu JW and Yuan Y: Pre-miR-27a rs895819A/G polymorphisms in cancer: a meta-analysis. PLoS One. 8:e652082013. View Article : Google Scholar : PubMed/NCBI

29 

Wang X, Wang ZH, Wu YY, et al: Melatonin attenuates scopolamine-induced memory/synaptic disorder by rescuing EPACs/miR-124/Egr1 pathway. Mol Neurobiol. 47:373–381. 2013. View Article : Google Scholar

30 

Lin XZ, Luo J, Zhang LP, et al: MiR-27a suppresses triglyceride accumulation and affects gene mRNA expression associated with fat metabolism in dairy goat mammary gland epithelial cells. Gene. 521:15–23. 2013. View Article : Google Scholar : PubMed/NCBI

31 

Chen L, Hu X, Dai Y, et al: MicroRNA-27a activity is not suppressed in porcine oocytes. Front Biosci (Elite Ed). 4:2679–2685. 2012. View Article : Google Scholar

32 

Fletcher CE, Dart DA, Sita-Lumsden A, et al: Androgen-regulated processing of the oncomir miR-27a, which targets Prohibitin in prostate cancer. Hum Mol Genet. 21:3112–3127. 2012. View Article : Google Scholar : PubMed/NCBI

33 

Yoon KA, Yoon H, Park S, et al: The prognostic impact of microRNA sequence polymorphisms on the recurrence of patients with completely resected non-small cell lung cancer. J Thorac Cardiovasc Surg. 144:794–807. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Hezova R, Kovarikova A, Bienertova-Vasku J, et al: Evaluation of SNPs in miR-196-a2, miR-27a and miR-146a as risk factors of colorectal cancer. World J Gastroenterol. 18:2827–2831. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Huang Z, Chen X, Yu B, He J and Chen D: MicroRNA-27a promotes myoblast proliferation by targeting myostatin. Biochem Biophys Res Commun. 423:265–269. 2012. View Article : Google Scholar : PubMed/NCBI

36 

Lü MH, Li CZ, Hu CJ, et al: microRNA-27b suppresses mouse MSC migration to the liver by targeting SDF-1α in vitro. Biochem Biophys Res Commun. 421:389–395. 2012. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Jiang Y, Duan Y and Zhou H: MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma. Oncol Lett 9: 471-477, 2015.
APA
Jiang, Y., Duan, Y., & Zhou, H. (2015). MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma. Oncology Letters, 9, 471-477. https://doi.org/10.3892/ol.2014.2701
MLA
Jiang, Y., Duan, Y., Zhou, H."MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma". Oncology Letters 9.1 (2015): 471-477.
Chicago
Jiang, Y., Duan, Y., Zhou, H."MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma". Oncology Letters 9, no. 1 (2015): 471-477. https://doi.org/10.3892/ol.2014.2701
Copy and paste a formatted citation
x
Spandidos Publications style
Jiang Y, Duan Y and Zhou H: MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma. Oncol Lett 9: 471-477, 2015.
APA
Jiang, Y., Duan, Y., & Zhou, H. (2015). MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma. Oncology Letters, 9, 471-477. https://doi.org/10.3892/ol.2014.2701
MLA
Jiang, Y., Duan, Y., Zhou, H."MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma". Oncology Letters 9.1 (2015): 471-477.
Chicago
Jiang, Y., Duan, Y., Zhou, H."MicroRNA‑27a directly targets KRAS to inhibit cell proliferation in esophageal squamous cell carcinoma". Oncology Letters 9, no. 1 (2015): 471-477. https://doi.org/10.3892/ol.2014.2701
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