MicroRNA let-7g and let-7i inhibit hepatoma cell growth concurrently via downregulation of the anti-apoptotic protein B-cell lymphoma-extra large

Wu,Lingjiao; Wang,Qiangfeng; Yao,Jian; Jiang,Han; Xiao,Cheng; Wu,Fusheng

doi:10.3892/ol.2014.2706

January-2015 Volume 9 Issue 1

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January-2015 Volume 9 Issue 1

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Article

MicroRNA let-7g and let-7i inhibit hepatoma cell growth concurrently via downregulation of the anti-apoptotic protein B-cell lymphoma-extra large

Authors:
- Lingjiao Wu
- Qiangfeng Wang
- Jian Yao
- Han Jiang
- Cheng Xiao
- Fusheng Wu
View Affiliations / Copyright

Affiliations: State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China, Department of Surgical Oncology, The First Affiliated Hospital, Zhejiang University, Hangzhou, Zhejiang 310003, P.R. China
Pages: 213-218
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Published online on: November 12, 2014

https://doi.org/10.3892/ol.2014.2706
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Abstract

Let‑7 family members have been identified as tumor‑suppressing microRNAs, which are important in human hepatocellular carcinoma (HCC). These family members may function differently as a result of different base sequences at the 3'end. The aim of this study was to determine the antitumor effects of miR‑let‑7g/i (let‑7g/i) on HCC cells and to investigate whether let‑7g and let‑7i have a combinatorial effect on HCC. The expression levels of let‑7g/i in hepatoma cells were determined by quantitative reverse transcription polymerase chain reaction. In addition, a 5‑ethynyl‑2'‑deoxyuridine retention assay and flow cytometry analysis were used to detect the effect of let‑7g/i on the proliferation and apoptosis of BEL‑7402 cells, respectively. The expression of anti‑apoptotic protein B‑cell lymphoma‑extra large (Bcl‑xL) was analyzed using western blot analysis. The results revealed that the expression levels of let‑7g/i were significantly decreased in HCC cell lines when compared with L‑02 cells. Furthermore, the overexpression of let‑7g/i significantly suppressed DNA replication, inhibited cell proliferation and promoted apoptosis of BEL‑7402 hepatoma cells. The expression of the anti‑apoptotic protein, Bcl‑xL, was inhibited by the combined role of let‑7g and let‑7i. We hypothesize that let‑7g and let‑7i exhibit a concurrent effect to regulate cell proliferation and the apoptosis of hepatoma cells, and this function is mediated by the Bcl‑xL protein.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

MicroRNA let-7g and let-7i inhibit hepatoma cell growth concurrently via downregulation of the anti-apoptotic protein B-cell lymphoma-extra large

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