Molecular analysis of human papillomavirus in never-smokers with non-small cell lung cancer

Isa,Shun‑Ichi; Kurahara,Yu; Yamamoto,Satomi; Tamiya,Akihiro; Omachi,Naoki; Asami,Kazuhiro; Okishio,Kyoichi; Utsumi,Tomoki; Ito,Norimasa; Yoon,Hyung‑Eun; Matsumura,Akihide; Atagi,Shinji; Kawaguchi,Tomoya

doi:10.3892/ol.2014.2713

February-2015 Volume 9 Issue 2

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February-2015 Volume 9 Issue 2

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Article

Molecular analysis of human papillomavirus in never-smokers with non-small cell lung cancer

Authors:
- Shun‑Ichi Isa
- Yu Kurahara
- Satomi Yamamoto
- Akihiro Tamiya
- Naoki Omachi
- Kazuhiro Asami
- Kyoichi Okishio
- Tomoki Utsumi
- Norimasa Ito
- Hyung‑Eun Yoon
- Akihide Matsumura
- Shinji Atagi
- Tomoya Kawaguchi
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Affiliations: Clinical Research Center, National Hospital Organization Kinki‑Chuo Chest Medical Center, Sakai, Osaka, Japan, Division of Internal Medicine, National Hospital Organization Kinki‑Chuo Chest Medical Center, Sakai, Osaka, Japan, Division of Chest Surgery, National Hospital Organization Kinki‑Chuo Chest Medical Center, Sakai, Osaka, Japan
Pages: 927-929
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Published online on: November 19, 2014

https://doi.org/10.3892/ol.2014.2713
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Abstract

The causes of lung cancer in never‑smokers remain unclear. The potential contribution of human papillomavirus (HPV) to the carcinogenesis of non‑small cell lung cancer (NSCLC) has been reported. In 2008, a prospective registry of never‑smokers with NSCLC was established at the Kinki‑Chuo Chest Medical Center, Sakai, Osaka, Japan. Never‑smokers with NSCLC were consecutively enrolled onto the registry. Of these patients, 114 with large tumor specimens, the majority of which were surgical tissues, were selected. In total, 23 of the most clinically relevant HPV types were assayed using polymerase chain reaction amplification of the viral genome. Following exclusion of samples with suboptimal quality, DNA was extracted from 96 formalin‑fixed paraffin‑embedded samples. These 96 cases consisted of 82 females (85.4%) and 14 males (14.6%), with a median age of 67 years (range, 29‑83). Almost all cases (93.8%) were of the adenocarcinoma histological subtype. Despite confirmation of the quality and amount of DNA, HPV type 6 was detected in only one case (1.1%). Furthermore, no other samples examined were positive for any other HPV types. The results therefore suggest that HPV does not play a major role as the driving oncogenic event in never‑smokers with NSCLC.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Molecular analysis of human papillomavirus in never-smokers with non-small cell lung cancer

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Abstract

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