Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
February-2015 Volume 9 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2015 Volume 9 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Piper betle extracts exhibit antitumor activity by augmenting antioxidant potential

  • Authors:
    • Badrul Alam
    • Rajib Majumder
    • Shahina Akter
    • Sang‑Han Lee
  • View Affiliations / Copyright

    Affiliations: Department of Food Science and Biotechnology, Kyungpook National University, Daegu 702‑701, Republic of Korea, Department of Pharmacy, Atish Dipankar University of Science and Technology, Dhaka 1213, Bangladesh
  • Pages: 863-868
    |
    Published online on: November 25, 2014
       https://doi.org/10.3892/ol.2014.2738
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study was conducted to evaluate the methanolic extract of Piper betle leaves (MPBL) and its organic fractions with regard to antitumor activity against Ehrlich ascites carcinoma (EAC) in Swiss albino mice and to confirm their antioxidant activities. At 24 h post‑intraperitoneal inoculation of tumor cells into mice, extracts were administered at 25, 50 and 100 mg/kg body weight for nine consecutive days. The antitumor effects of the extracts were then assessed according to tumor volume, packed cell count, viable and non‑viable tumor cell count, median survival time and increase in life span of EAC‑bearing mice. Next, hematological profiles and serum biochemical parameters were calculated, and antioxidant properties were assessed by estimating lipid peroxidation, reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT) levels. MPBL and the ethylacetate fraction (EPBL) at a dose of 100 mg/kg induced a significant decrease in tumor volume, packed cell volume and viable cell count and increased the life span of the EAC‑bearing mice (P<0.05). Hematological and serum biochemical profiles were restored to normal levels in the extract‑treated mice compared with the EAC control mice. MPBL and EPBL treatment significantly decreased lipid peroxidation (P<0.05) and restored GSH, SOD and CAT levels towards normal compared with the EAC control. Taken together, the results of the present study demonstrated that Piper betle extracts exhibit significant antitumor activity, which may be attributed to the augmentation of endogenous antioxidant potential.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Rajkumar V, Guha G and Kumar RA: Antioxidant and anti-neoplastic activities of Picrorhiza kurroa extracts. Food Chem Toxicol. 49:363–369. 2011. View Article : Google Scholar

2 

Suja KP, Jayalekshmy A and Arumughan C: Free radical scavenging behavior of antioxidant compounds of sesame (Sesamum indicum L.) in DPPH* system. J Agric Food Chem. 52:912–915. 2004. View Article : Google Scholar : PubMed/NCBI

3 

Meyskens FL Jr and Szabo E: Diet and cancer: the disconnect between epidemiology and randomized clinical trials. Cancer Epidemiol Biomarkers Prev. 14:1366–1369. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Murukami A, Ali AM, Mat-Salleh K, Koshimizu K and Ohigashi H: Screening for the in vitro anti-tumor-promoting activities of edible plants from Malaysia. Biosci Biotechnol Biochem. 64:9–16. 2000. View Article : Google Scholar

5 

Santhanam G and Nagarajan S: Wound healing activity of Curcuma aromatica and Piper betle. Fitoterapia. 61:458–459. 1990.

6 

Saravanan R, Prakasam A, Ramesh B and Pugalendi KV: Influence of Piper betle on hepatic marker enzymes and tissue antioxidant status in ethanol-treated Wistar rats. J Med Food. 5:197–204. 2002. View Article : Google Scholar

7 

Choudhary D and Kale RK: Antioxidant and non-toxic properties of Piper betle leaf extract: in vitro and in vivo studies. Phytother Res. 16:461–466. 2002. View Article : Google Scholar : PubMed/NCBI

8 

Bhide SV, Zariwala MB, Amonlar AJ and Azuine MA: Chemo-preventive efficacy of betel leaf extract against benzo[a]pyrene induced fore-stomach tumors in mice. J Ethnopharmacol. 34:207–213. 1991. View Article : Google Scholar : PubMed/NCBI

9 

Garg SC and Jain R: Biological activity of the essential oil of Piper betle L. J Essent oil Res. 23:601–606. 1992. View Article : Google Scholar

10 

Singh M, Shakya S, Soni VK, et al: The n-hexane and chloroform fractions of Piper betle L. trigger different arms of immune responses in BALB/c mice and exhibit antifilarial activity against human lymphatic filarid Brugia malayi. Int Immunopharmacol. 9:716–728. 2009. View Article : Google Scholar : PubMed/NCBI

11 

Zahan R, Alam MB, Islam MS, et al: Anticancer Activity of Alangium salviifolium flower in Ehrlich ascites carcinoma bearing mice. Int J Cancer Res. 7:254–262. 2011. View Article : Google Scholar

12 

Sur P, Bag SP and Khanam JA: Choroacetohydroxamic acid as antitumor agent against Ehrlich ascites carcinoma in mice. Neoplasma. 44:197–201. 1997.

13 

Gupta M, Mazumder UK, Kumar RS, et al: Antitumor activity and antioxidant status of Caesalpinia bonducella against Ehrlich ascites carcinoma in Swiss albino mice. J Pharmacol Sci. 94:177–184. 2004. View Article : Google Scholar : PubMed/NCBI

14 

Bergmeyer HU, Scelibe P and Wahlefeld AW: Optimization of methods for aspirate aminotransferase and alanine aminotransferase. Clin Chem. 24:58–73. 1978.PubMed/NCBI

15 

Malloy HT and Evelyn KA: The determination of bilirubin with the photometric colorimeter. J Biol Chem. 119:481–490. 1937.

16 

Lowry OH, Rosebrough NJ, Farr AL and Randall RJ: Protein measurement with the Folin-phenol reagent. J Biol Chem. 193:265–275. 1951.PubMed/NCBI

17 

Ohkawa H, Onishi N and Yagi K: Assay for lipid peroxidation in animal tissue by thiobarbituric acid reaction. Anal Biochem. 95:351–358. 1979. View Article : Google Scholar : PubMed/NCBI

18 

Ellman GL: Tissue sulfhydryl groups. Arch Biochem Biophys. 82:70–77. 1959. View Article : Google Scholar : PubMed/NCBI

19 

Pari L and Latha M: Protective role of Scoparia dulcis plant extract on brain antioxidant status and lipid peroxidation in STZ diabetic male Wistar rats. BMC Compliment Alternat Med. 4:162004. View Article : Google Scholar

20 

Segura JA, Barbero LG and Márquez J: Ehrlich ascites tumor unbalances splenic cell populations and reduced responsiveness of T cells to Staphylococcus aureus enterotoxin B stimulation. Immunomol Lett. 74:111–115. 2000. View Article : Google Scholar

21 

Opare Kennedy D, Kojima A, Hasuma T, et al: Growth inhibitory effects of green tea extract and (−)-epigallocatechin in Ehrlich ascites tumor cells involves a cellular thiol-dependent activation of mitogenic-activated protein kinases. Chem Biol Interac. 134:113–133. 2001. View Article : Google Scholar

22 

Blois MS: Antioxidant determination by the use of a stable free radical. Nature. 181:1199–1200. 1958. View Article : Google Scholar

23 

Jones PJ and AbuMweis SS: Phytosterols as functional food ingredients: linkages to cardiovascular disease and cancer. Curr Opin Clin Nutr Metabol Care. 12:147–151. 2009. View Article : Google Scholar

24 

Valko M, Leibfritz D, Moncol J, et al: Free radicals and antioxidants in normal physiological functions and human disease. Int J Biochem Cell Biol. 39:44–84. 2007. View Article : Google Scholar

25 

Valenzuela A: The biological significance of malondialdehyde determination in the assessment of tissue oxidative stress. Life Sci. 48:301–309. 1991. View Article : Google Scholar : PubMed/NCBI

26 

Yagi K: Lipid peroxides and human diseases. Chem Phys Lipids. 45:337–351. 1987. View Article : Google Scholar : PubMed/NCBI

27 

Szatrowski TP and Nathan CF: Production of large amounts of hydrogen peroxide by human tumor cells. Cancer Res. 51:794–798. 1991.PubMed/NCBI

28 

Seeram NP, Adams LS, Henning SM, et al: In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J Nutr Biochem. 16:360–367. 2005. View Article : Google Scholar : PubMed/NCBI

29 

Haldar PK, Kar B, Bala A, et al: Antitumor activity of Sansevieria roxburghiana rhizome against Ehrlich ascites carcinoma in mice. Pharm Biol. 48:1337–1343. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Rushmore TH and Picket CB: Glutathione S-transferases, structure, regulation and therapeutic implication. J Biol Chem. 268:11475–11478. 1993.PubMed/NCBI

31 

Marklund SL, Westman NG, Lundgren E and Roos G: Copper- and zinc-containing superoxide dismutase, manganese containing superoxide dismutase, catalase, and glutathione peroxidase in normal and neoplastic human cell lines and normal human tissues. Cancer Res. 42:1955–1961. 1982.PubMed/NCBI

32 

Sun Y, Oberley LW, Elwell JH and Sierra-Rivera E: Antioxidant enzyme activities in normal and transformed mice liver cells. Int J Cancer. 44:1028–1033. 1989. View Article : Google Scholar : PubMed/NCBI

33 

Ruby AJ, Kuttan G, Babu KD, Rajasekharan KN and Kuttan R: Anti-tumour and antioxidant activity of natural curcuminoids. Cancer Lett. 94:79–83. 1995. View Article : Google Scholar : PubMed/NCBI

34 

Rakshit S, Mandal L, Pal BC, et al: Involvement of ROS in chlorogenic acid-induced apoptosis of Bcr-Abl+ CML cells. Biochem Pharmacol. 80:1662–1675. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Alam B, Majumder R, Akter S and Lee SH: Piper betle extracts exhibit antitumor activity by augmenting antioxidant potential. Oncol Lett 9: 863-868, 2015.
APA
Alam, B., Majumder, R., Akter, S., & Lee, S. (2015). Piper betle extracts exhibit antitumor activity by augmenting antioxidant potential. Oncology Letters, 9, 863-868. https://doi.org/10.3892/ol.2014.2738
MLA
Alam, B., Majumder, R., Akter, S., Lee, S."Piper betle extracts exhibit antitumor activity by augmenting antioxidant potential". Oncology Letters 9.2 (2015): 863-868.
Chicago
Alam, B., Majumder, R., Akter, S., Lee, S."Piper betle extracts exhibit antitumor activity by augmenting antioxidant potential". Oncology Letters 9, no. 2 (2015): 863-868. https://doi.org/10.3892/ol.2014.2738
Copy and paste a formatted citation
x
Spandidos Publications style
Alam B, Majumder R, Akter S and Lee SH: Piper betle extracts exhibit antitumor activity by augmenting antioxidant potential. Oncol Lett 9: 863-868, 2015.
APA
Alam, B., Majumder, R., Akter, S., & Lee, S. (2015). Piper betle extracts exhibit antitumor activity by augmenting antioxidant potential. Oncology Letters, 9, 863-868. https://doi.org/10.3892/ol.2014.2738
MLA
Alam, B., Majumder, R., Akter, S., Lee, S."Piper betle extracts exhibit antitumor activity by augmenting antioxidant potential". Oncology Letters 9.2 (2015): 863-868.
Chicago
Alam, B., Majumder, R., Akter, S., Lee, S."Piper betle extracts exhibit antitumor activity by augmenting antioxidant potential". Oncology Letters 9, no. 2 (2015): 863-868. https://doi.org/10.3892/ol.2014.2738
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team