Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
February-2015 Volume 9 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
February-2015 Volume 9 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder

  • Authors:
    • Huayi Suo
    • Jia-Le Song
    • Yalin Zhou
    • Zhenhu Liu
    • Ruokun Yi
    • Kai Zhu
    • Jie Xie
    • Xin Zhao
  • View Affiliations / Copyright

    Affiliations: College of Food Science, Southwest University, Chongqing 400715, P.R. China, Department of Nutrition and Food Hygiene, School of Public Health, Guilin Medical University, Guilin, Guangxi 541004, P.R. China, Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China, Science and Technology Administration, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China, Department of Food Science and Nutrition, Pusan National University, Busan 609735, Republic of Korea, Department of Biological and Chemical Engineering, Chongqing University of Education, Chongqing 400067, P.R. China
  • Pages: 972-978
    |
    Published online on: December 2, 2014
       https://doi.org/10.3892/ol.2014.2756
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Larimichthys crocea swim bladder is a traditional food and medicine widely used in China. The in vitro anticancer effects of polysaccharide of L. crocea swim bladder (PLCSB) in HCT‑116 human colon cancer cells was investigated by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide assay. At concentrations ranging between 0 and 800 µg/ml PLCSB, cancer cell viability was decreased by PLCSB in a concentration‑dependent manner. In particular, 400 µg/ml PLCSB significantly (P<0.05) induced apoptosis, which was demonstrated by 4,6‑diamidino‑2‑phenylindole staining and flow cytometry analysis. To elucidate the mechanisms underlying the anticancer effect of PLCSB in HCT‑116 cancer cells, the expression of apoptosis and metastasis‑associated genes was analyzed by reverse transcription‑polymerase chain reaction and western blot analysis. A total of 400 µg/ml PLCSB significantly induced apoptosis in HCT‑116 cells (P<0.05) via the upregulation Bax, p53, p21, apoptotic protease activating factor 1, caspase‑3, ‑8, and ‑9, as well as Fas and the downregulation of B‑cell lymphoma 2 (Bcl‑2), Bcl‑extra large and Fas ligand (L). The results of this study demonstrated that PLCSB exhibits an anticancer effect on HCT‑116 colon cancer cells, in vitro.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

Li C and Yao CL: Molecular and expression characterizations of interleukin-8 gene in large yellow croaker (Larimichthys crocea). Fish Shellfish Immunol. 34:799–809. 2013. View Article : Google Scholar : PubMed/NCBI

2 

Lavi I, Friesem D, Geresh S, Hadar Y and Schwartz B: An aqueous polysaccharide extract from the edible mushroom Pleurotus ostreatus induces anti-proliferative and pro-apoptotic effects on HT-29 colon cancer cells. Cancer Lett. 244:61–70. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Li G, Kim DH, Kim TD, et al: Protein-bound polysaccharide from Phellinus linteus induces G2/M phase arrest and apoptosis in SW480 human colon cancer cells. Cancer Lett. 216:175–181. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Lowe SW and Lin AW: Apoptosis in cancer. Carcinogenesis. 21:485–495. 1999. View Article : Google Scholar

5 

Zhao X, Song JL, Wang Q, et al: Comparisons of Shuidouchi, Natto, and Cheonggukjang in their physicochemical properties, and antimutagenic and anticancer effects. Food Sci Biotechnol. 22:1077–1084. 2013. View Article : Google Scholar

6 

Efferth T, Li PC, Konkimalla VS and Kaina B: From traditional Chinese medicine to rational cancer therapy. Trends Mol Med. 13:353–361. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Yan C and Yan X: Study on extraction of Lycium barbarum polysaccharides by different methods and their antioxidant effects in vitro. Food Sci. 29:179–182. 2008.

8 

Zhao X, Deng XX, Park KY, Qiu L and Pang L: Purple bamboo salt has anticancer activity in TCA8113 cells in vitro and preventive effects on buccal mucosa cancer in mice in vivo. Exp Ther Med. 5:549–554. 2013.PubMed/NCBI

9 

Zhao X, Kim SY and Park KY: Bamboo salt has in vitro anticancer activity in HCT-116 cells and exerts anti-metastatic effects in vivo. J Med Food. 16:9–19. 2013. View Article : Google Scholar

10 

Zhao X, Ju JH, Kim HM and Park KY: Antimutagenic activity and in vitro anticancer effects of bamboo salt on HepG2 human hepatoma cells. J Environ Pathol Toxicol Oncol. 32:9–20. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Cao H, Tian XL and Liu X: Study on molecular identification and pharmacology of hemostasis action for isinglass. J Chinese Inst Food Sci Technol. 9:170–176. 2009.

12 

Chen S, Zhu K, Wang R and Zhao X: Preventive effect of polysaccharides from the large yellow croaker swim bladder on HCl/ethanol induced gastric injury in mice. Exp Ther Med. 8:316–322. 2014.PubMed/NCBI

13 

Jiang X, Zhao X, Luo H and Zhu K: Therapeutic effect of polysaccharide of large yellow croaker swim bladder on lupus nephritis of mice. Nutrients. 6:1223–1235. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Ashkenazi A and Dixit VM: Death receptors: signaling and modulation. Science. 281:1305–1308. 1998. View Article : Google Scholar : PubMed/NCBI

15 

Telford WG, King LE and Fraker PJ: Comparative evaluation of several DNA binding dyes in the detection of apoptosis associated chromatin degradation by flow cytometry. Cytometry. 13:137–143. 1992. View Article : Google Scholar

16 

Milanezi F, Leitão D, Ricardo S, Augusto I and Schmitt F: Evaluation of HER2 in breast cancer: reality and expectations. Expert Opin Med Diagn. 3:607–620. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Chao DT and Korsmeyer SJ: Bcl-2 family: regulators of cell death. Annu Rev Immunol. 16:395–419. 1998. View Article : Google Scholar : PubMed/NCBI

18 

Hengartner MO: The biochemistry of apoptosis. Nature. 407:770–776. 2000. View Article : Google Scholar : PubMed/NCBI

19 

Heiser D, Labi V, Erlacher M and Villunger A: The Bcl-2 protein family and its role in the development of neoplastic disease. Exp Gerontol. 39:1125–1135. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Miyashita T, Krajewski S, Krajewska M, et al: Tumor suppressor p53 is a regulator of bcl-2 and bax gene expression in vitro and in vivo. Oncogene. 9:1799–1805. 1994.PubMed/NCBI

21 

Miyashita T and Reed JC: Tumor suppressor p53 is a direct transcriptional activator of the human bax gene. Cell. 80:293–299. 1995. View Article : Google Scholar : PubMed/NCBI

22 

Rodriguez R and Meuth M: Chk1 and p21 cooperate to prevent apoptosis during DNA replication fork stress. Mol Biol Cell. 17:402–412. 2006. View Article : Google Scholar :

23 

Pop C, Timmer J, Sperandio S and Salvesen GS: The apoptosome activates caspase-9 by dimerization. Mol Cell. 22:269–275. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Ashkenazi A and Dixit VM: Death receptors: signaling and modulation. Science. 281:1305–1308. 1998. View Article : Google Scholar : PubMed/NCBI

25 

Cryns V and Yuan JY: Proteases to die for. Genes Dev. 12:1551–7150. 1998. View Article : Google Scholar : PubMed/NCBI

26 

Wajant H, Pfizenmaier K and Scheurich P: Non-apoptotic Fas signaling. Cytokine Growth Factor Rev. 14:53–66. 2003. View Article : Google Scholar

27 

Desagher S and Martinou JC: Mitochondria as the central control point of apoptosis. Trends Cell Biol. 10:369–377. 2000. View Article : Google Scholar : PubMed/NCBI

28 

Imao T and Nagata S: Apaf-1- and Caspase-8-independent apoptosis. Cell Death Differ. 20:343–352. 2013. View Article : Google Scholar :

29 

Zhu K, Li GJ, Sun P, et al: In vitro and in vivo anti-cancer activities of Kuding tea (Ilex kudingcha C.J. Tseng) against oral cancer. Exp Ther Med. 7:709–715. 2014.PubMed/NCBI

30 

Kim YA, Rhee SH, Park KY and Choi YH: Antiproliferative effect of resveratrol in human prostate carcinoma cells. J Med Food. 6:273–280. 2003. View Article : Google Scholar

31 

Choi S, Lew KL, Xiao H, et al: D, L-Sulforaphane-induced cell death in human prostate cancer cells is regulated by inhibitor of apoptosis family proteins and Apaf-1. Carcinogenesis. 28:151–162. 2007. View Article : Google Scholar

32 

Bush JA, Cheung KJ Jr and Li G: Curcumin induces apoptosis in human melanoma cells through a Fas receptor/caspase-8 pathway independent of p53. Exp Cell Res. 271:305–314. 2001. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Suo H, Song J, Zhou Y, Liu Z, Yi R, Zhu K, Xie J and Zhao X: Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder. Oncol Lett 9: 972-978, 2015.
APA
Suo, H., Song, J., Zhou, Y., Liu, Z., Yi, R., Zhu, K. ... Zhao, X. (2015). Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder. Oncology Letters, 9, 972-978. https://doi.org/10.3892/ol.2014.2756
MLA
Suo, H., Song, J., Zhou, Y., Liu, Z., Yi, R., Zhu, K., Xie, J., Zhao, X."Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder". Oncology Letters 9.2 (2015): 972-978.
Chicago
Suo, H., Song, J., Zhou, Y., Liu, Z., Yi, R., Zhu, K., Xie, J., Zhao, X."Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder". Oncology Letters 9, no. 2 (2015): 972-978. https://doi.org/10.3892/ol.2014.2756
Copy and paste a formatted citation
x
Spandidos Publications style
Suo H, Song J, Zhou Y, Liu Z, Yi R, Zhu K, Xie J and Zhao X: Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder. Oncol Lett 9: 972-978, 2015.
APA
Suo, H., Song, J., Zhou, Y., Liu, Z., Yi, R., Zhu, K. ... Zhao, X. (2015). Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder. Oncology Letters, 9, 972-978. https://doi.org/10.3892/ol.2014.2756
MLA
Suo, H., Song, J., Zhou, Y., Liu, Z., Yi, R., Zhu, K., Xie, J., Zhao, X."Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder". Oncology Letters 9.2 (2015): 972-978.
Chicago
Suo, H., Song, J., Zhou, Y., Liu, Z., Yi, R., Zhu, K., Xie, J., Zhao, X."Induction of apoptosis in HCT-116 colon cancer cells by polysaccharide of Larimichthys crocea swim bladder". Oncology Letters 9, no. 2 (2015): 972-978. https://doi.org/10.3892/ol.2014.2756
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team