Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
March-2015 Volume 9 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2015 Volume 9 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Quantitative detection of methylated NDRG4 gene as a candidate biomarker for diagnosis of colorectal cancer

  • Authors:
    • Wenhua Xiao
    • Huixia Zhao
    • Weiwei Dong
    • Quiwen Li
    • Jianhua Zhu
    • Guanghui Li
    • Shufang Zhang
    • Ming Ye
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of Chinese PLA General Hospital, Beijing 100048, P.R. China
  • Pages: 1383-1387
    |
    Published online on: December 19, 2014
       https://doi.org/10.3892/ol.2014.2815
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The present study explored the use of methylated NDRG4 gene as a candidate biomarker for diagnosis of colorectal cancer (CRC). Methylated NDRG4 gene expression from colorectal carcinoma tissue, paracarcinoma tissues, stools, blood and urine were detected successfully in DNA samples from 84 patients, by nested methylation‑specific polymerase chain reaction and denaturing high‑performance liquid chromatography. The sensitivity and specificity of methylated NDRG4 gene expression for us as a biomarker in colorectal cancer was analyzed and compared with 16 age‑matched healthy controls. The positive detection rate of methylated NDRG4 was 81% in carcinoma tissue, 8.3% in paracarcinoma tissues, 54.8% in blood, 72.6% in urine and 76.2% in stools. Considering the convenience of the acquisition of urine samples, an additional group of 76 patients with CRC were recruited for verification of detecting methylated NDRG4 in the urine. The positive detection rate of methylated NDRG4 was 72.4% (55/76) in this cohort. The detection of methylated NDRG4 in stools and urine could be used as a novel diagnostic technique for highly sensitive and specific detection of CRC. Due to the ease of collecting urine samples, this novel method could be a potential biomarker for early diagnosis of CRC.
View Figures

Figure 1

View References

1 

Todosi AM, Gavrilescu MM, Aniţei GM, et al: Colon cancer at the molecular level - usefulness of epithelial-mesenchymal transition analysis. Rev Med Chir Soc Med Nat Iasi. 116:1106–1111. 2012.

2 

Wallace PM and Suzuki R: Regional, racial, and gender differences in colorectal cancer screening in middle-aged African-Americans and Whites. J Cancer Educ. 27:703–708. 2012. View Article : Google Scholar : PubMed/NCBI

3 

Zauber AG, Winawer SJ, O’Brien MJ, et al: Colonoscopic polypectomy and long-term prevention of colorectal-cancer deaths. N Engl J Med. 366:687–696. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Nagasaka T, Mori Y, Umeda Y and Fujiwara T: Biomarker for colorectal cancer. Nihon Rinsho. 70:802–808. 2012.(In Japanese). PubMed/NCBI

5 

Imperiale TF, Ransohoff DF, Itzkowitz SH, et al: Fecal DNA versus fecal occult blood for colorectal-cancer screening in an average-risk population. N Engl J Med. 351:2704–2714. 2004. View Article : Google Scholar : PubMed/NCBI

6 

Sonnenberg A and Delcò F: Cost-effectiveness of a single colonoscopy in screening for colorectal cancer. Arch Intern Med. 162:163–168. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Lind GE, Danielsen SA, Ahlquist T, et al: Identification of an epigenetic biomarker panel with high sensitivity and specificity for colorectal cancer and adenomas. Mol Cancer. 10:852011. View Article : Google Scholar : PubMed/NCBI

8 

Mori Y, Olaru AV, Cheng Y, et al: Novel candidate colorectal cancer biomarkers identified by methylation microarray-based scanning. Endocr Relat Cancer. 18:465–478. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Jin X, Lin M, Zhang H, et al: Serum biomarkers of colorectal cancer with AU and NP20 chips including a diagnosis model. Hepatogastroenterology. 59:124–129. 2012.PubMed/NCBI

10 

Lenhard K, Bommer GT, Asutay S, et al: Analysis of promoter methylation in stool: a novel method for the detection of colorectal cancer. Clin Gastroenterol Hepatol. 3:142–149. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Lange CP, Campan M, Hinoue T, et al: Genome-scale discovery of DNA-methylation biomarkers for blood-based detection of colorectal cancer. PloS One. 7:e502662012. View Article : Google Scholar : PubMed/NCBI

12 

Cassinotti E, Melson J, Liggett T, et al: DNA methylation patterns in blood of patients with colorectal cancer and adenomatous colorectal polyps. Int J Cancer. 131:1153–1157. 2012. View Article : Google Scholar :

13 

Lange CP and Laird PW: Clinical applications of DNA methylation biomarkers in colorectal cancer. Epigenomics. 5:105–108. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Ahlquist DA, Zou H, Domanico M, et al: Next-generation stool DNA test accurately detects colorectal cancer and large adenomas. Gastroenterology. 142:248–256. 2012. View Article : Google Scholar

15 

Yamamoto H, Kokame K, Okuda T, et al: NDRG4 protein-deficient mice exhibit spatial learning deficits and vulnerabilities to cerebral ischemia. J Biol Chem. 286:26158–26165. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Melotte V, Lentjes MH, van den Bosch SM, et al: N-Myc downstream-regulated gene 4 (NDRG4): a candidate tumor suppressor gene and potential biomarker for colorectal cancer. J Natl Cancer Inst. 101:916–927. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Kotipatruni RP, Ferraro DJ, Ren X, et al: NDRG4, the N-Myc downstream regulated gene, is important for cell survival, tumor invasion and angiogenesis in meningiomas. Integr Biol (Camb). 4:1185–1197. 2012. View Article : Google Scholar

18 

Fan LP, Shen JZ, Ye BG, et al: Detection of p16 gene methylation status in adult patients with acute leukemia by using n-MSP. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 15:258–261. 2007.PubMed/NCBI

19 

Carraway HE, Wang S, Blackford A, et al: Promoter hypermethylation in sentinel lymph nodes as a marker for breast cancer recurrence. Breast Cancer Res Treat. 114:315–325. 2009. View Article : Google Scholar

20 

Ryan HE, Lo J and Johnson RS: HIF-1 alpha is required for solid tumor formation and embryonic vascularization. EMBO J. 17:3005–3015. 1998. View Article : Google Scholar : PubMed/NCBI

21 

Yi JM, Dhir M, Guzzetta AA, et al: DNA methylation biomarker candidates for early detection of colon cancer. Tumour Biol. 33:363–372. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Ziyab AH, Karmaus W, Holloway JW, et al: DNA methylation of the filaggrin gene adds to the risk of eczema associated with loss-of-function variants. J Eur Acad Dermatol Vernereol. 27:e420–e423. 2013. View Article : Google Scholar

23 

Morioka Y, Hibi K, Sakai M, et al: Aberrant methylation of the CHFR gene in digestive tract cancer. Anticancer Res. 26:1791–1795. 2006.PubMed/NCBI

24 

de la Chapelle A and Hampel H: Clinical relevance of microsatellite instability in colorectal cancer. J Clin Oncol. 28:3380–3387. 2010. View Article : Google Scholar : PubMed/NCBI

25 

Uehara E, Takeuchi S, Yang Y, et al: Aberrant methylation in promoter-associated CpG islands of multiple genes in chronic myelogenous leukemia blast crisis. Oncol Lett. 3:190–192. 2012.PubMed/NCBI

26 

Zhao YF, Zhang YG, Tian XX, Juan D and Jie Z: Aberrant methylation of multiple genes in gastric carcinomas. Int J Surg Pathol. 15:242–251. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Samejima K, Hiramatsu K, Takahashi K, et al: Identification and determination of urinary acetylpolyamines in cancer patients by electrospray ionization and time-of-flight mass spectrometry. Anal Biochem. 401:22–29. 2010. View Article : Google Scholar : PubMed/NCBI

28 

Cai H, Thomasset SC, P-Berry D, et al: Determination of anthocyanins in the urine of patients with colorectal liver metastases after administration of bilberry extract. Biomed Chromatogr. 25:660–663. 2011. View Article : Google Scholar

29 

Jackson C, Best N and Elliott P: UK Biobank Pilot Study: stability of haematological and clinical chemistry analytes. Int J Epidemiol. 37(Suppl 1): i16–i22. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Ahlquist DA, Taylor WR, Mahoney DW, et al: The stool DNA test is more accurate than the plasma septin 9 test in detecting colorectal neoplasia. Clin Gastroenterol Hepatol. 10:272–277. 2012. View Article : Google Scholar

31 

deVos T, Tetzner R, Model F, et al: Circulating methylated SEPT9 DNA in plasma is a biomarker for colorectal cancer. Clin Chem. 55:1337–1346. 2009. View Article : Google Scholar : PubMed/NCBI

32 

Lacks S and Greenberg B: Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation. J Mol Biol. 114:153–168. 1977. View Article : Google Scholar : PubMed/NCBI

33 

Hastings ML, Palma J and Duelli DM: Sensitive PCR-based quantitation of cell-free circulating microRNAs. Methods. 58:144–150. 2012. View Article : Google Scholar : PubMed/NCBI

34 

Ding W, Zhang J, Yoon JG, Shi D, Foltz G and Lin B: NDRG4 is downregulated in glioblastoma and inhibits cell proliferation. OMICS. 16:263–267. 2012. View Article : Google Scholar : PubMed/NCBI

35 

Yang X, An L and Li X: NDRG3 and NDRG4, two novel tumor-related genes. Biomed Pharmacother. 67:681–684. 2013. View Article : Google Scholar : PubMed/NCBI

36 

Hayashi T, Yoshikawa T, Bonam K, et al: The superiority of the seventh edition of the TNM classification depends on the overall survival of the patient cohort: comparative analysis of the sixth and seventh TNM editions in patients with gastric cancer from Japan and the United Kingdom. Cancer. 119:1330–1337. 2013. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Xiao W, Zhao H, Dong W, Li Q, Zhu J, Li G, Zhang S and Ye M: Quantitative detection of methylated NDRG4 gene as a candidate biomarker for diagnosis of colorectal cancer. Oncol Lett 9: 1383-1387, 2015.
APA
Xiao, W., Zhao, H., Dong, W., Li, Q., Zhu, J., Li, G. ... Ye, M. (2015). Quantitative detection of methylated NDRG4 gene as a candidate biomarker for diagnosis of colorectal cancer. Oncology Letters, 9, 1383-1387. https://doi.org/10.3892/ol.2014.2815
MLA
Xiao, W., Zhao, H., Dong, W., Li, Q., Zhu, J., Li, G., Zhang, S., Ye, M."Quantitative detection of methylated NDRG4 gene as a candidate biomarker for diagnosis of colorectal cancer". Oncology Letters 9.3 (2015): 1383-1387.
Chicago
Xiao, W., Zhao, H., Dong, W., Li, Q., Zhu, J., Li, G., Zhang, S., Ye, M."Quantitative detection of methylated NDRG4 gene as a candidate biomarker for diagnosis of colorectal cancer". Oncology Letters 9, no. 3 (2015): 1383-1387. https://doi.org/10.3892/ol.2014.2815
Copy and paste a formatted citation
x
Spandidos Publications style
Xiao W, Zhao H, Dong W, Li Q, Zhu J, Li G, Zhang S and Ye M: Quantitative detection of methylated NDRG4 gene as a candidate biomarker for diagnosis of colorectal cancer. Oncol Lett 9: 1383-1387, 2015.
APA
Xiao, W., Zhao, H., Dong, W., Li, Q., Zhu, J., Li, G. ... Ye, M. (2015). Quantitative detection of methylated NDRG4 gene as a candidate biomarker for diagnosis of colorectal cancer. Oncology Letters, 9, 1383-1387. https://doi.org/10.3892/ol.2014.2815
MLA
Xiao, W., Zhao, H., Dong, W., Li, Q., Zhu, J., Li, G., Zhang, S., Ye, M."Quantitative detection of methylated NDRG4 gene as a candidate biomarker for diagnosis of colorectal cancer". Oncology Letters 9.3 (2015): 1383-1387.
Chicago
Xiao, W., Zhao, H., Dong, W., Li, Q., Zhu, J., Li, G., Zhang, S., Ye, M."Quantitative detection of methylated NDRG4 gene as a candidate biomarker for diagnosis of colorectal cancer". Oncology Letters 9, no. 3 (2015): 1383-1387. https://doi.org/10.3892/ol.2014.2815
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team