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microRNA‑99a is downregulated and promotes proliferation, migration and invasion in non‑small cell lung cancer A549 and H1299 cells

  • Authors:
    • Changjin Chen
    • Ziyi Zhao
    • Yu Liu
    • Dezhi Mu

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  • Published online on: January 14, 2015     https://doi.org/10.3892/ol.2015.2873
  • Pages: 1128-1134

  • Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

There is increasing evidence that microRNAs (miRNAs) are able to play a key role in the diagnosis and therapy of cancer. miRNA‑99a (miR‑99a), which is downregulated in several human malignancies, has been reported as a potential tumor suppressor. However, to the best of our knowledge, the expression and function of miR‑99a has not been investigated in human non‑small cell lung cancer (NSCLC) at present. The aim of the current study was to evaluate the association between NSCLC and miR‑99a. miR‑99a expression was analyzed in 15 pairs of NSCLC and non‑cancerous tissue samples by reverse transcription‑quantitative polymerase chain reaction. In addition, the NSCLC A549 and H1299 cell lines were transfected with miR‑99a mimics, and the effect of miR‑99a on the cell cycle, cell proliferation, migration and colony formation of A549 and H1299 cells was investigated. It was found that the level of miR‑99a expression was significantly downregulated in NSCLC tissues and that ectopic overexpression of miR‑99a significantly inhibited the growth of A549 and H1299 cells. Additionally, ectopic overexpression of miR‑99a inhibited A549 and H1299 cell migration and invasion by inhibiting epithelial to mesenchymal transition. The downregulation of insulin‑like growth factor 1 receptor (IGF‑1R) by miR‑99a and knockdown of IGF‑1R mediated by siRNA were each found to phenocopy the effect of miR‑99a overexpression in NSCLC. To the best of our knowledge, the present study demonstrated for the first time that, in NSCLC, miR‑99a is downregulated and thus regulates proliferation, colony formation and migration through the IGF‑1R pathway, which indicates that miR‑99a is a diagnostic biomarker for NSCLC.
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March-2015
Volume 9 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen C, Zhao Z, Liu Y and Mu D: microRNA‑99a is downregulated and promotes proliferation, migration and invasion in non‑small cell lung cancer A549 and H1299 cells. Oncol Lett 9: 1128-1134, 2015
APA
Chen, C., Zhao, Z., Liu, Y., & Mu, D. (2015). microRNA‑99a is downregulated and promotes proliferation, migration and invasion in non‑small cell lung cancer A549 and H1299 cells. Oncology Letters, 9, 1128-1134. https://doi.org/10.3892/ol.2015.2873
MLA
Chen, C., Zhao, Z., Liu, Y., Mu, D."microRNA‑99a is downregulated and promotes proliferation, migration and invasion in non‑small cell lung cancer A549 and H1299 cells". Oncology Letters 9.3 (2015): 1128-1134.
Chicago
Chen, C., Zhao, Z., Liu, Y., Mu, D."microRNA‑99a is downregulated and promotes proliferation, migration and invasion in non‑small cell lung cancer A549 and H1299 cells". Oncology Letters 9, no. 3 (2015): 1128-1134. https://doi.org/10.3892/ol.2015.2873