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Article

FOXP1 regulation via the PI3K/Akt/p70S6K signaling pathway in breast cancer cells

  • Authors:
    • Sevil Oskay Halacli
    • Ayse Lale Dogan
  • View Affiliations / Copyright

    Affiliations: Pediatric Immunology Unit, Institute of Children's Health, Hacettepe University, Ankara 06100, Turkey, Department of Basic Oncology, Institute of Oncology, Hacettepe University, Ankara 06100, Turkey
  • Pages: 1482-1488
    |
    Published online on: January 16, 2015
       https://doi.org/10.3892/ol.2015.2885
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Abstract

Loss of Forkhead box P1 (FOXP1) protein expression confers a poor prognosis in sporadic and familial breast cancer patients, and the FOXP1 gene maps to a tumor suppressor locus at chromosome 3p14. Although correlation studies have indicated that FOXP1 has a role in tumor suppression, determination of the regulatory mechanism of FOXP1 is required to establish its function in breast cancer. It has previously been identified that FOXP1 is regulated by estrogen in breast cancer and that treatment with bisphenol A is effective for regulating the transformation of the normal human breast epithelial cell line, MCF‑10F. In addition, FOXO‑regulated activation of FOXP1 inhibits the apoptosis of MCF‑10F cells following tamoxifen and Akt inhibitor VIII administration. The present study indicates that FOXP1 regulation occurs via a PI3K/Akt/p70S6 kinase (p70S6K) signaling pathway. Following treatment with wortmannin, an inhibitor of phosphatidylinositol 3‑kinase (PI3K)/Akt, MCF7 and MDA‑MB‑231 breast cancer cells demonstrated decreased FOXP1 protein expression levels; this result was also observed in the small interfering (si)RNA silencing of Akt. By contrast, overexpression of Akt resulted in increased FOXP1 protein expression levels in the MDA‑MB‑231 cells compared with the control cell lysates. Furthermore, treatment with rapamycin, a specific inhibitor of the mammalian target of rapamycin/p70S6K cascade, resulted in decreased FOXP1 expression in the MCF7 cells, but not in the MDA‑MB‑231 cells, which were resistant to rapamycin‑induced inhibition. In addition, silencing of p70S6K using siRNA produced a marked decrease in FOXP1 expression. These data indicate that FOXP1 protein expression is regulated by a PI3K/Akt/p70S6K signaling cascade in breast cancer.
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Copy and paste a formatted citation
Spandidos Publications style
Halacli SO and Dogan AL: FOXP1 regulation via the PI3K/Akt/p70S6K signaling pathway in breast cancer cells. Oncol Lett 9: 1482-1488, 2015.
APA
Halacli, S.O., & Dogan, A.L. (2015). FOXP1 regulation via the PI3K/Akt/p70S6K signaling pathway in breast cancer cells. Oncology Letters, 9, 1482-1488. https://doi.org/10.3892/ol.2015.2885
MLA
Halacli, S. O., Dogan, A. L."FOXP1 regulation via the PI3K/Akt/p70S6K signaling pathway in breast cancer cells". Oncology Letters 9.3 (2015): 1482-1488.
Chicago
Halacli, S. O., Dogan, A. L."FOXP1 regulation via the PI3K/Akt/p70S6K signaling pathway in breast cancer cells". Oncology Letters 9, no. 3 (2015): 1482-1488. https://doi.org/10.3892/ol.2015.2885
Copy and paste a formatted citation
x
Spandidos Publications style
Halacli SO and Dogan AL: FOXP1 regulation via the PI3K/Akt/p70S6K signaling pathway in breast cancer cells. Oncol Lett 9: 1482-1488, 2015.
APA
Halacli, S.O., & Dogan, A.L. (2015). FOXP1 regulation via the PI3K/Akt/p70S6K signaling pathway in breast cancer cells. Oncology Letters, 9, 1482-1488. https://doi.org/10.3892/ol.2015.2885
MLA
Halacli, S. O., Dogan, A. L."FOXP1 regulation via the PI3K/Akt/p70S6K signaling pathway in breast cancer cells". Oncology Letters 9.3 (2015): 1482-1488.
Chicago
Halacli, S. O., Dogan, A. L."FOXP1 regulation via the PI3K/Akt/p70S6K signaling pathway in breast cancer cells". Oncology Letters 9, no. 3 (2015): 1482-1488. https://doi.org/10.3892/ol.2015.2885
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