Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
April-2015 Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2015 Volume 9 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Identification of differentially‑expressed genes by DNA methylation in cervical cancer

  • Authors:
    • Heun‑Sik Lee
    • Jun Ho Yun
    • Junghee Jung
    • Young Yang
    • Bong‑Jo Kim
    • Sung‑Jong Lee
    • Joo Hee Yoon
    • Yong Moon
    • Jeong‑Min Kim
    • Yong‑Il Kwon
  • View Affiliations / Copyright

    Affiliations: Center for Genome Science, Korea National Institute of Health, Osong Health Technology Administration Complex, Cheongju, Chungcheongbuk‑do 363‑951, Republic of Korea, Macrogen Inc., Seoul 153‑023, Republic of Korea, Center for Women's Disease, Department of Biological Science, Sookmyung Women's University, Seoul 140‑742, Republic of Korea, Department of Obstetrics and Gynecology, Saint Vincent's Hospital, the Catholic University, Suwon, Gyeonggi-do 442‑723, Republic of Korea, Department of Public Health Administration, Namseoul University, Cheonan, Chungcheongnam-do 331‑707, Republic of Korea, Department of Obstetrics and Gynecology, Kangdong Sacred Heart Hospital, Hallym University Medical Center, Seoul 134‑701, Republic of Korea
  • Pages: 1691-1698
    |
    Published online on: January 29, 2015
       https://doi.org/10.3892/ol.2015.2917
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

To identify novel cervical cancer‑related genes that are regulated by DNA methylation, integrated analyses of genome‑wide DNA methylation and RNA expression profiles were performed using the normal and tumor regions of tissues from four patients; two with cervical cancer and two with pre‑invasive cancer. The present study identified 19 novel cervical cancer‑related genes showing differential RNA expression by DNA methylation. A number of the identified genes were novel cervical cancer‑related genes and their differential expression was confirmed in a publicly available database. Among the candidate genes, the epigenetic regulation and expression of three genes, CAMK2N1, ALDH1A3 and PPP1R3C, was validated in HeLa cells treated with a demethylating reagent using methylation‑specific polymerase chain reaction (PCR) and quantitative PCR, respectively. From these results, the expression of the CAMK2N1, ALDH1A3 and PPP1R3C genes are were shown to be suppressed in cervical cancers by DNA methylation. These genes may be involved in the progression or initiation of cervical cancer.
View Figures

Figure 1

Figure 2

View References

1 

Forouzanfar MH, Foreman KJ, Delossantos AM, et al: Breast and cervical cancer in 187 countries between 1980 and 2010: a systematic analysis. Lancet. 378:1461–1484. 2011. View Article : Google Scholar : PubMed/NCBI

2 

Woodman CB, Collins SI and Young LS: The natural history of cervical HPV infection: unresolved issues. Nat Rev Cancer. 7:11–22. 2007. View Article : Google Scholar

3 

Jensen KE, Schmiedel S, Frederiksen K, et al: Risk for cervical intraepithelial neoplasia grade 3 or worse in relation to smoking among women with persistent human papillomavirus infection. Cancer Epidemiol Biomarkers Prev. 21:1949–1955. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Werness BA, Levine AJ and Howley PM: Association of human papillomavirus types 16 and 18 E6 proteins with p53. Science. 248:76–79. 1990. View Article : Google Scholar : PubMed/NCBI

5 

Dyson N, Howley PM, Münger K and Harlow E: The human papilloma virus-16 E7 oncoprotein is able to bind to the retinoblastoma gene product. Science. 243:934–937. 1989. View Article : Google Scholar : PubMed/NCBI

6 

Whiteside MA, Siegel EM and Unger ER: Human papillomavirus and molecular considerations for cancer risk. Cancer. 113(10 Suppl): 2981–2994. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Esteller M: Epigenetics in cancer. N Engl J Med. 358:1148–1159. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Feinberg AP and Vogelstein B: Hypomethylation distinguishes genes of some human cancers from their normal counterparts. Nature. 301:89–92. 1983. View Article : Google Scholar : PubMed/NCBI

9 

Herman JG, Merlo A, Mao L, et al: Inactivation of the CDKN2/p16/MTS1 gene is frequently associated with aberrant DNA methylation in all common human cancers. Cancer Res. 55:4525–4530. 1995.PubMed/NCBI

10 

Silva TD, Vidigal VM, Felipe AV, et al: DNA methylation as an epigenetic biomarker in colorectal cancer. Oncol Lett. 6:1687–1692. 2013.PubMed/NCBI

11 

Jeong DH, Youm MY, Kim YN, et al: Promoter methylation of p16, DAPK, CDH1, and TIMP-3 genes in cervical cancer: correlation with clinicopathologic characteristics. Int J Gynecol Cancer. 16:1234–1240. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Neyaz MK, Kumar RS, Hussain S, et al: Effect of aberrant promoter methylation of FHIT and RASSF1A genes on susceptibility to cervical cancer in a North Indian population. Biomarkers. 13:597–606. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Lendvai A, Johannes F, Grimm C, et al: Genome-wide methylation profiling identifies hypermethylated biomarkers in high-grade cervical intraepithelial neoplasia. Epigenetics. 7:1268–1278. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Sun Z, Asmann YW, Kalari KR, et al: Integrated analysis of gene expression, CpG island methylation, and gene copy number in breast cancer cells by deep sequencing. PLoS One. 6:e174902011. View Article : Google Scholar : PubMed/NCBI

15 

Li M, Balch C, Montgomery JS, et al: Integrated analysis of DNA methylation and gene expression reveals specific signaling pathways associated with platinum resistance in ovarian cancer. BMC Med Genomics. 2:342009. View Article : Google Scholar : PubMed/NCBI

16 

Irizarry RA, Ladd-Acosta C, Carvalho B, et al: Comprehensive high-throughput arrays for relative methylation (CHARM). Genome Res. 18:780–790. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Agarwal SM, Raghav D, Singh H and Raghava GP: CCDB: a curated database of genes involved in cervix cancer. Nucleic Acids Res. 39:D975–979. 2011. View Article : Google Scholar :

18 

Shin G, Kang TW, Yang S, et al: GENT: gene expression database of normal and tumor tissues. Cancer Inform. 10:149–157. 2011.PubMed/NCBI

19 

Eisen MB, Spellman PT, Brown PO and Botstein D: Cluster analysis and display of genome-wide expression patterns. Proc Natl Acad Sci USA. 95:14863–14868. 1998. View Article : Google Scholar : PubMed/NCBI

20 

Huang da W, Sherman BT, Tan Q, et al: The DAVID Gene functional classification tool: a novel biological module-centric algorithm to functionally analyze large gene lists. Genome Biol. 8:R1832007. View Article : Google Scholar : PubMed/NCBI

21 

Kim JM, Sohn HY, Yoon SY, et al: Identification of gastric cancer-related genes using a cDNA microarray containing novel expressed sequence tags expressed in gastric cancer cells. Clin Cancer Res. 11:473–482. 2005.PubMed/NCBI

22 

Li LC and Dahiya R: MethPrimer: designing primers for methylation PCRs. Bioinformatics. 18:1427–1431. 2002. View Article : Google Scholar : PubMed/NCBI

23 

Chao A, Wang TH, Lee YS, et al: Molecular characterization of adenocarcinoma and squamous carcinoma of the uterine cervix using microarray analysis of gene expression. Int J Cancer. 119:91–98. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Song JY, Lee JK, Lee NW, et al: Microarray analysis of normal cervix, carcinoma in situ, and invasive cervical cancer: identification of candidate genes in pathogenesis of invasion in cervical cancer. Int J Gynecol Cancer. 18:1051–1059. 2008. View Article : Google Scholar : PubMed/NCBI

25 

Kavanagh E, Tsapara A, Matter K and Balda MS: Tight junctions and the regulation of epithelial cell proliferation and gene expression. Tight Junctions. Springer; pp. 101–115. 2006, View Article : Google Scholar

26 

Togami S, Nomoto M, Higashi M, et al: Expression of mucin antigens (MUC1 and MUC16) as a prognostic factor for mucinous adenocarcinoma of the uterine cervix. J Obstet Gynaecol Res. 36:588–597. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Munro EG, Jain M, Oliva E, et al: Upregulation of MUC4 in cervical squamous cell carcinoma: pathologic significance. Int J Gynecol Pathol. 28:127–133. 2009. View Article : Google Scholar : PubMed/NCBI

28 

Shames DS, Girard L, Gao B, et al: A genome-wide screen for promoter methylation in lung cancer identifies novel methylation markers for multiple malignancies. PLoS Med. 3:e4862006. View Article : Google Scholar : PubMed/NCBI

29 

Zhang W, Yan W, You G, et al: Genome-wide DNA methylation profiling identifies ALDH1A3 promoter methylation as a prognostic predictor in G-CIMP-primary glioblastoma. Cancer Lett. 328:120–125. 2013. View Article : Google Scholar

30 

Wong YF, Cheung TH, Tsao GS, et al: Genome-wide gene expression profiling of cervical cancer in Hong Kong women by oligonucleotide microarray. Int J Cancer. 118:2461–2469. 2006. View Article : Google Scholar

31 

Bonazzi VF, Irwin D and Hayward NK: Identification of candidate tumor suppressor genes inactivated by promoter methylation in melanoma. Genes Chromosomes Cancer. 48:10–21. 2009. View Article : Google Scholar

32 

Kim JH, Dhanasekaran SM, Prensner JR, et al: Deep sequencing reveals distinct patterns of DNA methylation in prostate cancer. Genome Res. 21:1028–1041. 2011. View Article : Google Scholar : PubMed/NCBI

33 

Wang C, Li N, Liu X, Zheng Y and Cao X: A novel endogenous human CaMKII inhibitory protein suppresses tumor growth by inducing cell cycle arrest via p27 stabilization. J Biol Chem. 283:11565–11574. 2008. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Lee HS, Yun JH, Jung J, Yang Y, Kim BJ, Lee SJ, Yoon JH, Moon Y, Kim JM, Kwon YI, Kwon YI, et al: Identification of differentially‑expressed genes by DNA methylation in cervical cancer. Oncol Lett 9: 1691-1698, 2015.
APA
Lee, H., Yun, J.H., Jung, J., Yang, Y., Kim, B., Lee, S. ... Kwon, Y. (2015). Identification of differentially‑expressed genes by DNA methylation in cervical cancer. Oncology Letters, 9, 1691-1698. https://doi.org/10.3892/ol.2015.2917
MLA
Lee, H., Yun, J. H., Jung, J., Yang, Y., Kim, B., Lee, S., Yoon, J. H., Moon, Y., Kim, J., Kwon, Y."Identification of differentially‑expressed genes by DNA methylation in cervical cancer". Oncology Letters 9.4 (2015): 1691-1698.
Chicago
Lee, H., Yun, J. H., Jung, J., Yang, Y., Kim, B., Lee, S., Yoon, J. H., Moon, Y., Kim, J., Kwon, Y."Identification of differentially‑expressed genes by DNA methylation in cervical cancer". Oncology Letters 9, no. 4 (2015): 1691-1698. https://doi.org/10.3892/ol.2015.2917
Copy and paste a formatted citation
x
Spandidos Publications style
Lee HS, Yun JH, Jung J, Yang Y, Kim BJ, Lee SJ, Yoon JH, Moon Y, Kim JM, Kwon YI, Kwon YI, et al: Identification of differentially‑expressed genes by DNA methylation in cervical cancer. Oncol Lett 9: 1691-1698, 2015.
APA
Lee, H., Yun, J.H., Jung, J., Yang, Y., Kim, B., Lee, S. ... Kwon, Y. (2015). Identification of differentially‑expressed genes by DNA methylation in cervical cancer. Oncology Letters, 9, 1691-1698. https://doi.org/10.3892/ol.2015.2917
MLA
Lee, H., Yun, J. H., Jung, J., Yang, Y., Kim, B., Lee, S., Yoon, J. H., Moon, Y., Kim, J., Kwon, Y."Identification of differentially‑expressed genes by DNA methylation in cervical cancer". Oncology Letters 9.4 (2015): 1691-1698.
Chicago
Lee, H., Yun, J. H., Jung, J., Yang, Y., Kim, B., Lee, S., Yoon, J. H., Moon, Y., Kim, J., Kwon, Y."Identification of differentially‑expressed genes by DNA methylation in cervical cancer". Oncology Letters 9, no. 4 (2015): 1691-1698. https://doi.org/10.3892/ol.2015.2917
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team