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Article

Detection of CD133 expression in U87 glioblastoma cells using a novel anti-CD133 monoclonal antibody

  • Authors:
    • Dongyang Wang
    • Yuanxu Guo
    • Yanqing Li
    • Weiling Li
    • Xiaojing Zheng
    • Haibin Xia
    • Qinwen Mao
  • View Affiliations / Copyright

    Affiliations: Co‑Innovation Center for Qinba Regions' Sustainable Development, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710062, P.R. China, Department of Pathology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA
  • Pages: 2603-2608
    |
    Published online on: March 27, 2015
       https://doi.org/10.3892/ol.2015.3079
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Abstract

In glioblastomas, the surface glycoprotein CD133 (prominin-1) indicates the presence of cancer stem cells (CSCs), which are able to initiate tumor growth and are highly resistant to conventional chemo/radiotherapy. However, a number of studies have reported that certain CD133‑ glioma cells are able to self‑renew and retain tumorigenic potential. In addition, the reliability of CD133 as a CSC marker is controversial due to inconsistent findings with regard to the prognostic values and distribution of CD133. Such controversies may be due to the detection limits using currently available anti‑CD133 antibodies. In the present study, novel anti‑human CD133 monoclonal antibodies (mAbs) were generated using two recombinant extracellular domains of human CD133: CD133 ectodomain 1 (amino acids 171‑420) and CD133 ectodomain 2 (amino acids 507‑716). One of the antibodies produced against CD133 ectodomain 2, C2E1, detected high expression levels of CD133 protein in glioblastoma U87 cells, in contrast to previous studies which did not detect CD133 expression in these cells. The cells exhibited a cytoplasmic distribution pattern of CD133 and produced a 95 kDa band following western blot analysis. In addition, C2E1 was able to bind the full‑length glycosylated CD133 on the cell surface and inhibit the proliferation of tumor cells. Therefore, this antibody may be a valuable tool to study CD133 as a CSC marker and may be significant in future cancer treatments.
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Copy and paste a formatted citation
Spandidos Publications style
Wang D, Guo Y, Li Y, Li W, Zheng X, Xia H and Mao Q: Detection of CD133 expression in U87 glioblastoma cells using a novel anti-CD133 monoclonal antibody. Oncol Lett 9: 2603-2608, 2015.
APA
Wang, D., Guo, Y., Li, Y., Li, W., Zheng, X., Xia, H., & Mao, Q. (2015). Detection of CD133 expression in U87 glioblastoma cells using a novel anti-CD133 monoclonal antibody. Oncology Letters, 9, 2603-2608. https://doi.org/10.3892/ol.2015.3079
MLA
Wang, D., Guo, Y., Li, Y., Li, W., Zheng, X., Xia, H., Mao, Q."Detection of CD133 expression in U87 glioblastoma cells using a novel anti-CD133 monoclonal antibody". Oncology Letters 9.6 (2015): 2603-2608.
Chicago
Wang, D., Guo, Y., Li, Y., Li, W., Zheng, X., Xia, H., Mao, Q."Detection of CD133 expression in U87 glioblastoma cells using a novel anti-CD133 monoclonal antibody". Oncology Letters 9, no. 6 (2015): 2603-2608. https://doi.org/10.3892/ol.2015.3079
Copy and paste a formatted citation
x
Spandidos Publications style
Wang D, Guo Y, Li Y, Li W, Zheng X, Xia H and Mao Q: Detection of CD133 expression in U87 glioblastoma cells using a novel anti-CD133 monoclonal antibody. Oncol Lett 9: 2603-2608, 2015.
APA
Wang, D., Guo, Y., Li, Y., Li, W., Zheng, X., Xia, H., & Mao, Q. (2015). Detection of CD133 expression in U87 glioblastoma cells using a novel anti-CD133 monoclonal antibody. Oncology Letters, 9, 2603-2608. https://doi.org/10.3892/ol.2015.3079
MLA
Wang, D., Guo, Y., Li, Y., Li, W., Zheng, X., Xia, H., Mao, Q."Detection of CD133 expression in U87 glioblastoma cells using a novel anti-CD133 monoclonal antibody". Oncology Letters 9.6 (2015): 2603-2608.
Chicago
Wang, D., Guo, Y., Li, Y., Li, W., Zheng, X., Xia, H., Mao, Q."Detection of CD133 expression in U87 glioblastoma cells using a novel anti-CD133 monoclonal antibody". Oncology Letters 9, no. 6 (2015): 2603-2608. https://doi.org/10.3892/ol.2015.3079
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