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Expression changes of cell‑cell adhesion‑related genes in colorectal tumors

  • Authors:
    • Mateusz Bujko
    • Paulina Kober
    • Michal Mikula
    • Marcin Ligaj
    • Jerzy Ostrowski
    • Janusz Aleksander Siedlecki
  • View Affiliations / Copyright

    Affiliations: Department of Molecular and Translational Oncology, Maria Sklodowska‑Curie Memorial Cancer Center and Institute of Oncology, Warsaw 02‑781, Poland, Department of Genetics, Maria Sklodowska‑Curie Memorial Cancer Center and Institute of Oncology, Warsaw 02‑781, Poland, Department of Pathology, Maria Sklodowska‑Curie Memorial Cancer Center and Institute of Oncology, Warsaw 02‑781, Poland
    Copyright: © Bujko et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2463-2470
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    Published online on: April 8, 2015
       https://doi.org/10.3892/ol.2015.3107
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Abstract

Epithelial tissues achieve a highly organized structure due to cell‑cell junction complexes. Carcinogenesis is accompanied by changes in cell interactions and tissue morphology, which appear in the early stages of benign tumors and progress along with invasive potential. The aim of the present study was to analyze the changes in expression levels of genes encoding intercellular junction proteins that have been previously identified to be differentially expressed in colorectal tumors compared with normal mucosa samples (fold change, >2.5) in genome‑wide expression profiling. The expression of 20 selected genes was assessed using quantitative reverse transcription polymerase chain reaction in 26 colorectal cancer, 42 adenoma and 24 normal mucosa samples. Between these tissue types, differences were observed in the mRNA levels of genes encoding adherens junction proteins (upregulation of CDH3 and CDH11, and downregulation of CDH19 and PTPRF), tight junction proteins (upregulation of CLDN1 and CLDN2, and downregulation of CLDN5, CLDN8, CLDN23, CLDN15, JAM2 and CGN) and desmosomes (upregulation of DSC3 and DSG3, and downregulation of DSC2), in addition to a decrease in the expression of certain other genes involved in intercellular connections: PCDHB14, PCDH7, MUPCDH and NEO1. The differences between tissue types were statistically significant, and separate clustering of normal adenoma and carcinoma samples was observed in a hierarchical clustering analysis. These results indicate that the morphological changes in neoplastic colon tissue that occur during the ‘adenoma‑carcinoma sequence’ are accompanied by specific changes in the expression of multiple genes encoding the majority of cell‑cell junction complexes. The particular differential expression patterns appear to be consistent among patients with cancer and adenoma, in addition to normal mucosa samples.
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Copy and paste a formatted citation
Spandidos Publications style
Bujko M, Kober P, Mikula M, Ligaj M, Ostrowski J and Siedlecki JA: Expression changes of cell‑cell adhesion‑related genes in colorectal tumors. Oncol Lett 9: 2463-2470, 2015.
APA
Bujko, M., Kober, P., Mikula, M., Ligaj, M., Ostrowski, J., & Siedlecki, J.A. (2015). Expression changes of cell‑cell adhesion‑related genes in colorectal tumors. Oncology Letters, 9, 2463-2470. https://doi.org/10.3892/ol.2015.3107
MLA
Bujko, M., Kober, P., Mikula, M., Ligaj, M., Ostrowski, J., Siedlecki, J. A."Expression changes of cell‑cell adhesion‑related genes in colorectal tumors". Oncology Letters 9.6 (2015): 2463-2470.
Chicago
Bujko, M., Kober, P., Mikula, M., Ligaj, M., Ostrowski, J., Siedlecki, J. A."Expression changes of cell‑cell adhesion‑related genes in colorectal tumors". Oncology Letters 9, no. 6 (2015): 2463-2470. https://doi.org/10.3892/ol.2015.3107
Copy and paste a formatted citation
x
Spandidos Publications style
Bujko M, Kober P, Mikula M, Ligaj M, Ostrowski J and Siedlecki JA: Expression changes of cell‑cell adhesion‑related genes in colorectal tumors. Oncol Lett 9: 2463-2470, 2015.
APA
Bujko, M., Kober, P., Mikula, M., Ligaj, M., Ostrowski, J., & Siedlecki, J.A. (2015). Expression changes of cell‑cell adhesion‑related genes in colorectal tumors. Oncology Letters, 9, 2463-2470. https://doi.org/10.3892/ol.2015.3107
MLA
Bujko, M., Kober, P., Mikula, M., Ligaj, M., Ostrowski, J., Siedlecki, J. A."Expression changes of cell‑cell adhesion‑related genes in colorectal tumors". Oncology Letters 9.6 (2015): 2463-2470.
Chicago
Bujko, M., Kober, P., Mikula, M., Ligaj, M., Ostrowski, J., Siedlecki, J. A."Expression changes of cell‑cell adhesion‑related genes in colorectal tumors". Oncology Letters 9, no. 6 (2015): 2463-2470. https://doi.org/10.3892/ol.2015.3107
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