Acquisition of 5-fluorouracil resistance induces epithelial-mesenchymal transitions through the Hedgehog signaling pathway in HCT-8 colon cancer cells

  • Authors:
    • Yanjun Liu
    • Fangfang Du
    • Qiannan Zhao
    • Jian Jin
    • Xin Ma
    • Huazhong Li
  • View Affiliations

  • Published online on: April 23, 2015     https://doi.org/10.3892/ol.2015.3136
  • Pages: 2675-2679
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Abstract

Colon cancer has a high incidence in individuals >60-years-old. The commonly used chemotherapeutic agent, 5-fluorouracil (5-FU), has gradually lost its potency in treating colorectal cancer following the acquisition of resistance. Drug resistance is usually associated with epithelial‑mesenchymal transitions (EMTs) in cancer cells. In the present study, the EMT phenotypes of two colon cancer cell lines, wild‑type (HCT‑8/WT) and 5‑FU‑resistant (HCT‑8/5‑FU), were characterized following the analysis of cellular migration, proliferation, morphology and molecular changes. In order to further clarify the mechanism of EMT in HCT‑8/5‑FU cells, the effect of EMT pathway inhibitors upon drug sensitivity was investigated. The results revealed that the Hedgehog signaling pathway inhibitor, GDC0449, reversed drug resistance. Therefore, inhibition of the Hedgehog pathway may provide a novel chemotherapeutic strategy for the treatment of patients with 5-FU-resistant colon cancer.
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June-2015
Volume 9 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Liu Y, Du F, Zhao Q, Jin J, Ma X and Li H: Acquisition of 5-fluorouracil resistance induces epithelial-mesenchymal transitions through the Hedgehog signaling pathway in HCT-8 colon cancer cells. Oncol Lett 9: 2675-2679, 2015
APA
Liu, Y., Du, F., Zhao, Q., Jin, J., Ma, X., & Li, H. (2015). Acquisition of 5-fluorouracil resistance induces epithelial-mesenchymal transitions through the Hedgehog signaling pathway in HCT-8 colon cancer cells. Oncology Letters, 9, 2675-2679. https://doi.org/10.3892/ol.2015.3136
MLA
Liu, Y., Du, F., Zhao, Q., Jin, J., Ma, X., Li, H."Acquisition of 5-fluorouracil resistance induces epithelial-mesenchymal transitions through the Hedgehog signaling pathway in HCT-8 colon cancer cells". Oncology Letters 9.6 (2015): 2675-2679.
Chicago
Liu, Y., Du, F., Zhao, Q., Jin, J., Ma, X., Li, H."Acquisition of 5-fluorouracil resistance induces epithelial-mesenchymal transitions through the Hedgehog signaling pathway in HCT-8 colon cancer cells". Oncology Letters 9, no. 6 (2015): 2675-2679. https://doi.org/10.3892/ol.2015.3136