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Guggulsterone inhibits human cholangiocarcinoma Sk-ChA-1 and Mz-ChA-1 cell growth by inducing caspase-dependent apoptosis and downregulation of survivin and Bcl-2 expression

  • Authors:
    • Fei Zhong
    • Jing Yang
    • Zhu-Ting Tong
    • Liu-Liu Chen
    • Lu-Lu Fan
    • Fang Wang
    • Xia-Li Zha
    • Jun Li
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China, Department of Neurology, Anhui Provincial Hospital, Hefei, Anhui 230001, P.R. China, Department of Radiation Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, P.R. China
    Copyright: © Zhong et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1416-1422
    |
    Published online on: June 19, 2015
       https://doi.org/10.3892/ol.2015.3391
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Abstract

Guggulsterone has recently been reported to demonstrate anti-tumor effects in a variety of cancers. The present study aims to investigate the biological roles and underlying mechanism of the action of guggulsterone in cholangiocarcinoma. The immortalized human cholangiocarcinoma Sk‑ChA‑1 and Mz‑ChA‑1 cell lines were treated with various concentrations of the trans isomer of guggulsterone, Z‑guggulsterone. Cellular proliferation was determined using the XTT assay. The apoptotic status of cholangiocarcinoma cells was assessed by Hoechst 33258 staining, DNA fragmentation assay and flow cytometry. Specific caspase inhibitor was used to explore the role of caspase in guggulsterone‑induced apoptosis. A colorimetric assay was performed to measure the alterations of the activities of caspase‑3, ‑8 and ‑9. Western blot analysis was used to detect the protein expression of survivin, B‑cell lymphoma 2 (Bcl‑2), Bcl‑2‑associated X protein and cleaved poly (adenosine diphosphate‑ribose) polymerase (PARP). As revealed by the present data, guggulsterone significantly inhibited the growth of the two human cholangiocarcinoma cell lines by inducing cellular apoptosis. In addition, guggulsterone‑induced apoptosis of cholangiocarcinoma cells was demonstrated to be partially inhibited by the caspase inhibitors z‑VAD‑fmk, z‑LEHD‑fmk and z‑IETD‑fmk, accompanied by the activation of caspases‑3, ‑8 and ‑9, accumulation of cleaved PARP and decreased expression of survivin and Bcl‑2. In conclusion, the present study demonstrated that guggulsterone was able to suppress the proliferation of cholangiocarcinoma by inducing caspase‑dependent apoptosis and downregulating survivin and Bcl-2.
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View References

1 

Lazaridis KN and Gores GJ: Cholangiocarcinoma. Gastroenterology. 128:1655–1667. 2005. View Article : Google Scholar : PubMed/NCBI

2 

West J, Wood H, Logan RF, Quinn M and Aithal GP: Trends in the incidence of primary liver and biliary tract cancers in England and Wales 1971–2001. Br J Cancer. 94:1751–1758. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Anderson CD, Pinson CW, Berlin J and Chari RS: Diagnosis and treatment of cholangiocarcinoma. Oncologist. 9:43–57. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Sirica AE: Cholangiocarcinoma: Molecular targeting strategies for chemoprevention and therapy. Hepatology. 41:5–15. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Fulda S, Gorman AM, Hori O and Samali A: Cellular stress responses: Cell survival and cell death. Int J Cell Biol. 2010:2140742010. View Article : Google Scholar : PubMed/NCBI

6 

Fabregat I, Roncero C and Fernández M: Survival and apoptosis: A dysregulated balance in liver cancer. Liver Int. 27:155–162. 2007. View Article : Google Scholar : PubMed/NCBI

7 

Ferreira CG, Epping M, Kruyt FA and Giaccone G: Apoptosis: Target of cancer therapy. Clin Cancer Res. 8:2024–2034. 2002.PubMed/NCBI

8 

Kim ND, Im E, Yoo YH and Choi YH: Modulation of the cell cycle and induction of apoptosis in human cancer cells by synthetic bile acids. Curr Cancer Drug Targets. 6:681–689. 2006. View Article : Google Scholar : PubMed/NCBI

9 

Gujral ML, Sareen K, Tangri KK, Amma MK and Roy AK: Antiarthritic and anti-inflammatory activity of gum guggul (Balsamodendron mukul Hook). Indian J Physiol Pharmacol. 4:267–273. 1960.PubMed/NCBI

10 

Sharma JN and Sharma JN: Comparison of the anti-inflammatory activity of Commiphora mukul (an indigenous drug) with those of phenylbutazone and ibuprofen in experimental arthritis induced by mycobacterial adjuvant. Arzneimittelforschung. 27:1455–1457. 1977.PubMed/NCBI

11 

Urizar NL and Moore DD: GUGULIPID: A natural cholesterol-lowering agent. Annu Rev Nutr. 23:303–313. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Urizar NL, Liverman AB, Dodds DT, Silva FV, Ordentlich P, Yan Y, Gonzalez FJ, Heyman RA, Mangelsdorf DJ and Moore DD: A natural product that lowers cholesterol as an antagonist ligand for FXR. Science. 296:1703–1706. 2002. View Article : Google Scholar : PubMed/NCBI

13 

Singh SV, Choi S, Zeng Y, Hahm ER and Xiao D: Guggulsterone-induced apoptosis in human prostate cancer cells is caused by reactive oxygen intermediate dependent activation of c-Jun NH2 -terminal kinase. Cancer Res. 67:7439–7449. 2007. View Article : Google Scholar : PubMed/NCBI

14 

Shishodia S, Sethi G, Ahn KS and Aggarwal BB: Guggulsterone inhibits tumor cell proliferation, induces S-phase arrest, and promotes apoptosis through activation of c-Jun N-terminal kinase, suppression of Akt pathway, and downregulation of antiapoptotic gene products. Biochem Pharmacol. 74:118–130. 2007. View Article : Google Scholar : PubMed/NCBI

15 

Samudio I, Konopleva M, Safe S, McQueen T and Andreeff M: Guggulsterones induce apoptosis and differentiation in acute myeloid leukemia: Identification of isomer-specific antileukemic activities of the pregnadienedione structure. Mol Cancer Ther. 4:1982–1992. 2005. View Article : Google Scholar : PubMed/NCBI

16 

An MJ, Cheon JH, Kim SW, Kim ES, Kim TI and Kim WH: Guggulsterone induces apoptosis in colon cancer cells and inhibits tumor growth in murine colorectal cancer xenografts. Cancer Letters. 279:93–100. 2009. View Article : Google Scholar : PubMed/NCBI

17 

Macha MA, Rachagani S, Gupta S, Pai P, Ponnusamy MP, Batra SK and Jain M: Guggulsterone decreases proliferation and metastatic behavior of pancreatic cancer cells by modulating JAK/STAT and Src/FAK signaling. Cancer Lett. 341:166–177. 2013. View Article : Google Scholar : PubMed/NCBI

18 

Guan B, Li H, Yang Z, Hoque A and Xu X: Inhibition of farnesoid X receptor controls esophageal cancer cell growth in vitro and in nude mouse xenografts. Cancer. 119:1321–1329. 2013. View Article : Google Scholar : PubMed/NCBI

19 

Herrmann M, Lorenz HM, Voll R, Grünke M, Woith W and Kalden JR: A rapid and simple method for the isolation of apoptotic DNA fragments. Nucleic Acids Res. 22:5506–5507. 1994. View Article : Google Scholar : PubMed/NCBI

20 

Kanjeekal S, Chambers A, Fung MF and Verma S: Systemic therapy for advanced uterine sarcoma: A systematic review of the literature. Gynecol Oncol. 97:624–637. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Wiseman LR and Spencer CM: Paclitaxel. An update of its use in the treatment of metastatic breast cancer and ovarian and other gynaecological cancers. Drugs Aging. 12:305–334. 1998. View Article : Google Scholar : PubMed/NCBI

22 

Jiang J, Liang X, Zhou X, Huang L, Huang R, Chu Z and Zhan Q: A meta-analysis of randomized controlled trials comparing irinotecan/platinum with etoposide/platinum in patients with previously untreated extensive-stage small cell lung cancer. J Thorac Oncol. 5:867–873. 2010. View Article : Google Scholar : PubMed/NCBI

23 

Karikas GA: Anticancer and chemopreventing natural products: Some biochemical and therapeutic aspects. J BUON. 15:627–638. 2010.PubMed/NCBI

24 

Reed JC: Apoptosis-regulating proteins as targets for drug discovery. Trends Mol Med. 7:314–319. 2001. View Article : Google Scholar : PubMed/NCBI

25 

Desagher S and Martinou JC: Mitochondria as the central control point of apoptosis. Trends Cell Biol. 10:369–377. 2000. View Article : Google Scholar : PubMed/NCBI

26 

Van Loo G, Saelens X, Van Gurp M, MacFarlane M, Martin SJ and Vandenabeele P: The role of mitochondrial factors in apoptosis: A Russian roulette with more than one bullet. Cell Death Differ. 9:1031–1042. 2002. View Article : Google Scholar : PubMed/NCBI

27 

Thornberry NA and Lazebnik Y: Caspases: Enemies within. Science. 281:1312–1316. 1998. View Article : Google Scholar : PubMed/NCBI

28 

Fulda S: Targeting extrinsic apoptosis in cancer: Challenges and opportunities. Semin Cell Dev Biol. 39:20–25. 2015. View Article : Google Scholar : PubMed/NCBI

29 

Liang Q, Li W and Zhou B: Caspase-independent apoptosis in yeast. Biochim Biophys Acta. 1783:1311–1319. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Susin SA, Lorenzo HK, Zamzami N, Marzo I, Snow BE, Brothers GM, Mangion J, Jacotot E, Costantini P, Loeffler M, et al: Molecular characterization of mitochondrial apoptosis-inducing factor. Nature. 397:441–446. 1999. View Article : Google Scholar : PubMed/NCBI

31 

Li F: Survivin study: What is the next wave? J Cell Physiol. 197:8–29. 2003. View Article : Google Scholar : PubMed/NCBI

32 

Li F, Ambrosini G, Chu EY, Plescia J, Tognin S, Marchisio PC and Altieri DC: Control of apoptosis and mitotic spindle checkpoint by survivin. Nature. 396:580–584. 1998. View Article : Google Scholar : PubMed/NCBI

33 

Mirza A, McGuirk M, Hockenberry TN, Wu Q, Ashar H, Black S, Wen SF, Wang L, Kirschmeier P, Bishop WR, et al: Human survivin is negatively regulated by wild-type p53 and participates in p53-dependent apoptotic pathway. Oncogene. 21:2613–2622. 2002. View Article : Google Scholar : PubMed/NCBI

34 

Beltrami E, Plescia J, Wilkinson JC, Duckett CS and Altieri DC: Acute ablation of survivin uncovers p53-dependent mitotic checkpoint functions and control of mitochondrial apoptosis. J Biol Chem. 279:2077–2084. 2004. View Article : Google Scholar : PubMed/NCBI

35 

Chang Q, Liu ZR, Wang DY, Kumar M, Chen YB and Qin RY: Survivin expression induced by doxorubicin in cholangiocarcinoma. World J Gastroenterol. 10:415–418. 2004.PubMed/NCBI

36 

Javle MM, Tan D, Yu J, LeVea CM, Li F, Kuvshinoff BW and Gibbs JF: Nuclear survivin expression predicts poor outcome in cholangiocarcinoma. Hepatogastroenterol. 51:1653–1657. 2004.

37 

Obama K, Ura K, Li M, Katagiri T, Tsunoda T, Nomura A, Satoh S, Nakamura Y and Furukawa Y: Genome-wide analysis of gene expression in human intrahepatic cholangiocarcinoma. Hepatology. 41:1339–1348. 2005. View Article : Google Scholar : PubMed/NCBI

38 

Antonsson B: Mitochondria and the Bcl-2 family proteins in apoptosis signaling pathways. Mol Cell Biochem. 256–257:141–155. 2004. View Article : Google Scholar

39 

Nascimento Pde S, Ornellas AA, Campos MM, Scheiner MA, Fiedler W and Alves G: Bax and bcl-2 imbalance and HPB infection in penile tumors and adjacent tissues. Prog Urol. 14:353–359. 2004.(In French). PubMed/NCBI

40 

Reed JC: Double identity for proteins of the Bcl-2 family. Nature. 387:773–776. 1997. View Article : Google Scholar : PubMed/NCBI

41 

Wei MC, Zong WX, Cheng EH, Lindsten T, Panoutsakopoulou V, Ross AJ, MacGregor GR, Thompson CB and Korsmeyer SJ: Proapoptotic BAX and BAK: A requisite gateway to mitochondrial dysfunction and death. Science. 292:727–730. 2001. View Article : Google Scholar : PubMed/NCBI

42 

Zhang CL, Wu LJ, Tashiro S, Onodera S and Ikejima T: Oridonin induces apoptosis of HeLa cells via altering expression of Bcl-2/Bax and activating caspase-3/ICAD pathway. Acta Pharmacol Sin. 25:691–698. 2004.PubMed/NCBI

43 

Manion MK and Hockenbery DM: Targeting BCL-2-related proteins in cancer therapy. Cancer Biol Ther. 2((Sul 1)): S105–S114. 2003.PubMed/NCBI

44 

Kanwar JR, Kamalapuram SK and Kanwar RK: Targeting survivin in cancer: The cell-signalling perspective. Drug Discov Today. 16:485–494. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhong F, Yang J, Tong Z, Chen L, Fan L, Wang F, Zha X and Li J: Guggulsterone inhibits human cholangiocarcinoma Sk-ChA-1 and Mz-ChA-1 cell growth by inducing caspase-dependent apoptosis and downregulation of survivin and Bcl-2 expression. Oncol Lett 10: 1416-1422, 2015.
APA
Zhong, F., Yang, J., Tong, Z., Chen, L., Fan, L., Wang, F. ... Li, J. (2015). Guggulsterone inhibits human cholangiocarcinoma Sk-ChA-1 and Mz-ChA-1 cell growth by inducing caspase-dependent apoptosis and downregulation of survivin and Bcl-2 expression. Oncology Letters, 10, 1416-1422. https://doi.org/10.3892/ol.2015.3391
MLA
Zhong, F., Yang, J., Tong, Z., Chen, L., Fan, L., Wang, F., Zha, X., Li, J."Guggulsterone inhibits human cholangiocarcinoma Sk-ChA-1 and Mz-ChA-1 cell growth by inducing caspase-dependent apoptosis and downregulation of survivin and Bcl-2 expression". Oncology Letters 10.3 (2015): 1416-1422.
Chicago
Zhong, F., Yang, J., Tong, Z., Chen, L., Fan, L., Wang, F., Zha, X., Li, J."Guggulsterone inhibits human cholangiocarcinoma Sk-ChA-1 and Mz-ChA-1 cell growth by inducing caspase-dependent apoptosis and downregulation of survivin and Bcl-2 expression". Oncology Letters 10, no. 3 (2015): 1416-1422. https://doi.org/10.3892/ol.2015.3391
Copy and paste a formatted citation
x
Spandidos Publications style
Zhong F, Yang J, Tong Z, Chen L, Fan L, Wang F, Zha X and Li J: Guggulsterone inhibits human cholangiocarcinoma Sk-ChA-1 and Mz-ChA-1 cell growth by inducing caspase-dependent apoptosis and downregulation of survivin and Bcl-2 expression. Oncol Lett 10: 1416-1422, 2015.
APA
Zhong, F., Yang, J., Tong, Z., Chen, L., Fan, L., Wang, F. ... Li, J. (2015). Guggulsterone inhibits human cholangiocarcinoma Sk-ChA-1 and Mz-ChA-1 cell growth by inducing caspase-dependent apoptosis and downregulation of survivin and Bcl-2 expression. Oncology Letters, 10, 1416-1422. https://doi.org/10.3892/ol.2015.3391
MLA
Zhong, F., Yang, J., Tong, Z., Chen, L., Fan, L., Wang, F., Zha, X., Li, J."Guggulsterone inhibits human cholangiocarcinoma Sk-ChA-1 and Mz-ChA-1 cell growth by inducing caspase-dependent apoptosis and downregulation of survivin and Bcl-2 expression". Oncology Letters 10.3 (2015): 1416-1422.
Chicago
Zhong, F., Yang, J., Tong, Z., Chen, L., Fan, L., Wang, F., Zha, X., Li, J."Guggulsterone inhibits human cholangiocarcinoma Sk-ChA-1 and Mz-ChA-1 cell growth by inducing caspase-dependent apoptosis and downregulation of survivin and Bcl-2 expression". Oncology Letters 10, no. 3 (2015): 1416-1422. https://doi.org/10.3892/ol.2015.3391
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