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Article

PU.1 affects proliferation of the human acute myeloid leukemia U937 cell line by directly regulating MEIS1

  • Authors:
    • Jing Zhou
    • Xiaofeng Zhang
    • Yuhua Wang
    • Yinghui Guan
  • View Affiliations / Copyright

    Affiliations: Laboratory of Genome Variations and Precision Bio‑Medicine, Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 100101, P.R. China, Department of Chemistry, University of Massachusetts Boston, Boston, MA 02125, USA, Department of Immunology and Infectious Diseases, The Forsyth Institute, Cambridge, MA 02142, USA, Respiratory Department, 2nd Branch of First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 1912-1918
    |
    Published online on: June 22, 2015
       https://doi.org/10.3892/ol.2015.3404
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Abstract

The transcription factor PU.1 is a member of the ETS family, which is expressed in a wide variety of hematopoietic lineages. Accumulating evidence has indicated that PU.1 plays a key role in hematopoiesis, and reduced expression of PU.1 leads to the pathogenesis of human myeloid leukemia. As a multi‑functional factor, PU.1 is also required for mixed lineage leukemia (MLL) stem cell potential and the development of MLL. However, the function of PU.1 in human non‑MLL leukemia and its molecular mechanism remains poorly understood. In the present study, PU.1 siRNA was demonstrated to efficiently inhibit the transcription level of oncogene MEIS1 in the human acute myeloid non‑MLL leukemia U937 cell line. In addition, PU.1, as a positive regulator of MEIS1, performed a crucial role in maintaining cell proliferation. Using electrophoretic mobility shift assay, chromatin immunoprecipitation analysis and luciferase reporter assay, previously unexplored evidence that PU.1 activated the MEIS1 promoter through a conserved binding motif in vitro and in vivo was further defined. Overall, the present study provides insight into the molecular mechanism of the contribution of PU.1 to the pathogenesis of non‑MLL U937 cells, which is mediated by direct regulation of MEIS1 transcription. The present data reveal the possibility of developing an alternative therapy for non‑MLL leukemia by targeting PU.1-mediated MEIS1 gene activation.
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1 

Orkin SH and Zon LI: Hematopoiesis: An evolving paradigm for stem cell biology. Cell. 132:631–644. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Kehrl JH: Hematopoietic lineage commitment: role of transcription factors. Stem Cells. 13:223–241. 1995. View Article : Google Scholar : PubMed/NCBI

3 

Gangenahalli GU, Gupta P, Saluja D, Verma YK, Kishore V, Chandra R, Sharma RK and Ravindranath T: Stem cell fate specification: Role of master regulatory switch transcription factor PU.1 in differential hematopoiesis. Stem Cells Dev. 14:140–152. 2005. View Article : Google Scholar : PubMed/NCBI

4 

Aikawa Y, Katsumoto T, Zhang P, Shima H, Shino M, Terui K, Ito E, Ohno H, Stanley ER, Singh H, et al: PU.1-mediated upregulation of CSF1R is crucial for leukemia stem cell potential induced by MOZ-TIF2. Nat Med. 16:580–585, 1p following 585. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Houston IB, Huang KJ, Jennings SR and DeKoter RP: PU.1 immortalizes hematopoietic progenitors in a GM-CSF-dependent manner. Exp Hematol. 35:374–384. 2007. View Article : Google Scholar : PubMed/NCBI

6 

Mak KS, Funnell AP, Pearson RC and Crossley M: PU.1 and Haematopoietic Cell Fate: Dosage Matters. Int J Cell Biol. 2011:8085242011. View Article : Google Scholar : PubMed/NCBI

7 

Friedman AD: Transcriptional control of granulocyte and monocyte development. Oncogene. 26:6816–6828. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Iwasaki H, Somoza C, Shigematsu H, Duprez EA, Iwasaki-Arai J, Mizuno S, Arinobu Y, Geary K, Zhang P, Dayaram T, et al: Distinctive and indispensable roles of PU.1 in maintenance of hematopoietic stem cells and their differentiation. Blood. 106:1590–1600. 2005. View Article : Google Scholar : PubMed/NCBI

9 

Nutt SL, Metcalf D, D'Amico A, Polli M and Wu L: Dynamic regulation of PU.1 expression in multipotent hematopoietic progenitors. J Exp Med. 201:221–231. 2005. View Article : Google Scholar : PubMed/NCBI

10 

Renneville A, Roumier C, Biggio V, et al: Cooperating gene mutations in acute myeloid leukemia: A review of the literature. Leukemia. 22:915–931. 2008. View Article : Google Scholar : PubMed/NCBI

11 

Seshire A, Rößiger T, Frech M, et al: Direct interaction of PU.1 with oncogenic transcription factors reduces its serine phosphorylation and promoter binding. Leukemia. 26:1338–1347. 2012. View Article : Google Scholar : PubMed/NCBI

12 

Mueller BU, Pabst T, Fos J, et al: ATRA resolves the differentiation block in t (15;17) acute myeloid leukemia by restoring PU.1 expression. Blood. 107:3330–3338. 2006. View Article : Google Scholar : PubMed/NCBI

13 

Vangala RK, Heiss-Neumann MS, Rangatia JS, et al: The myeloid master regulator transcription factor PU.1 is inactivated by AML1-ETO in t (8;21) myeloid leukemia. Blood. 101:270–277. 2003. View Article : Google Scholar : PubMed/NCBI

14 

Wang K, Wang P, Shi J, et al: PML/RARalpha targets promoter regions containing PU.1 consensus and RARE half sites in acute promyelocytic leukemia. Cancer Cell. 17:186–197. 2010. View Article : Google Scholar : PubMed/NCBI

15 

Staber PB, Zhang P, Ye M, et al: Sustained PU.1 levels balance cell-cycle regulators to prevent exhaustion of adult hematopoietic stem cells. Mol Cell. 49:934–946. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Zhou J, Wu J, Li B, et al: PU.1 is essential for MLL leukemia partially via crosstalk with the MEIS/HOX pathway. Leukemia. 28:1436–1448. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Kumar AR, Li Q, Hudson WA, Chen W, Sam T, Yao Q, Lund EA, Wu B, Kowal BJ and Kersey JH: A role for MEIS1 in MLL-fusion gene leukemia. Blood. 113:1756–1758. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Dahl R, Iyer SR, Owens KS, Cuylear DD and Simon MC: The transcriptional repressor GFI-1 antagonizes PU.1 activity through protein-protein interaction. J Biol Chem. 282:6473–6483. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Zeisig BB, Milne T, García-Cuéllar MP, Schreiner S, Martin ME, Fuchs U, Borkhardt A, Chanda SK, Walker J, Soden R, et al: Hoxa9 and Meis1 are key targets for MLL-ENL-mediated cellular immortalization. Mol Cell Biol. 24:617–628. 2004. View Article : Google Scholar : PubMed/NCBI

20 

Hu YL, Fong S, Ferrell C, Largman C and Shen WF: HOXA9 modulates its oncogenic partner Meis1 to influence normal hematopoiesis. Mol Cell Biol. 29:5181–5192. 2009. View Article : Google Scholar : PubMed/NCBI

21 

Xiang P, Lo C, Argiropoulos B, et al: Identification of E74-like factor 1 (ELF1) as a transcriptional regulator of the Hox cofactor MEIS1. Exp Hematol. 38:798, 808 e1-e2. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Orlovsky K, Kalinkovich A, Rozovskaia T, et al: Down-regulation of homeobox genes MEIS1 and HOXA in MLL-rearranged acute leukemia impairs engraftment and reduces proliferation. Proc Natl Acad Sci USA. 108:7956–7961. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Wong P, Iwasaki M, Somervaille TC, So CW and Cleary ML: Meis1 is an essential and rate-limiting regulator of MLL leukemia stem cell potential. Genes Dev. 21:2762–2774. 2007. View Article : Google Scholar : PubMed/NCBI

24 

Gupta P, Gurudutta GU, Saluja D and Tripathi RP: PU.1 and partners: Regulation of haematopoietic stem cell fate in normal and malignant haematopoiesis. J Cell Mol Med. 13:4349–4363. 2009. View Article : Google Scholar : PubMed/NCBI

25 

Dakic A, Metcalf D, Di Rago L, Mifsud S, Wu L and Nutt SL: PU.1 regulates the commitment of adult hematopoietic progenitors and restricts granulopoiesis. J Exp Med. 201:1487–1502. 2005. View Article : Google Scholar : PubMed/NCBI

26 

Rosenbauer F, Wagner K, Kutok JL, et al: Acute myeloid leukemia induced by graded reduction of a lineage-specific transcription factor, PU.1. Nat Genet. 36:624–630. 2004. View Article : Google Scholar : PubMed/NCBI

27 

Suraweera N, Meijne E, Moody J, Carvajal-Carmona LG, Yoshida K, Pollard P, Fitzgibbon J, Riches A, van Laar T, Huiskamp R, et al: Mutations of the PU.1 Ets domain are specifically associated with murine radiation-induced, but not human therapy-related, acute myeloid leukaemia. Oncogene. 24:3678–3683. 2005. View Article : Google Scholar : PubMed/NCBI

28 

Walter MJ, Park JS, Ries RE, Lau SK, McLellan M, Jaeger S, Wilson RK, Mardis ER and Ley TJ: Reduced PU.1 expression causes myeloid progenitor expansion and increased leukemia penetrance in mice expressing PML-RARalpha. Proc Natl Acad Sci USA. 102:12513–12518. 2005. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhou J, Zhang X, Wang Y and Guan Y: PU.1 affects proliferation of the human acute myeloid leukemia U937 cell line by directly regulating MEIS1. Oncol Lett 10: 1912-1918, 2015.
APA
Zhou, J., Zhang, X., Wang, Y., & Guan, Y. (2015). PU.1 affects proliferation of the human acute myeloid leukemia U937 cell line by directly regulating MEIS1. Oncology Letters, 10, 1912-1918. https://doi.org/10.3892/ol.2015.3404
MLA
Zhou, J., Zhang, X., Wang, Y., Guan, Y."PU.1 affects proliferation of the human acute myeloid leukemia U937 cell line by directly regulating MEIS1". Oncology Letters 10.3 (2015): 1912-1918.
Chicago
Zhou, J., Zhang, X., Wang, Y., Guan, Y."PU.1 affects proliferation of the human acute myeloid leukemia U937 cell line by directly regulating MEIS1". Oncology Letters 10, no. 3 (2015): 1912-1918. https://doi.org/10.3892/ol.2015.3404
Copy and paste a formatted citation
x
Spandidos Publications style
Zhou J, Zhang X, Wang Y and Guan Y: PU.1 affects proliferation of the human acute myeloid leukemia U937 cell line by directly regulating MEIS1. Oncol Lett 10: 1912-1918, 2015.
APA
Zhou, J., Zhang, X., Wang, Y., & Guan, Y. (2015). PU.1 affects proliferation of the human acute myeloid leukemia U937 cell line by directly regulating MEIS1. Oncology Letters, 10, 1912-1918. https://doi.org/10.3892/ol.2015.3404
MLA
Zhou, J., Zhang, X., Wang, Y., Guan, Y."PU.1 affects proliferation of the human acute myeloid leukemia U937 cell line by directly regulating MEIS1". Oncology Letters 10.3 (2015): 1912-1918.
Chicago
Zhou, J., Zhang, X., Wang, Y., Guan, Y."PU.1 affects proliferation of the human acute myeloid leukemia U937 cell line by directly regulating MEIS1". Oncology Letters 10, no. 3 (2015): 1912-1918. https://doi.org/10.3892/ol.2015.3404
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