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Article

Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase‑2 expression

  • Authors:
    • Kun Sun
    • Xiao‑He Tang
    • Yi‑Kui Xie
  • View Affiliations / Copyright

    Affiliations: Department of Gastroenterology, The First Hospital of Zibo, Zibo, Shandong 255200, P.R. China
  • Pages: 1649-1654
    |
    Published online on: June 25, 2015
       https://doi.org/10.3892/ol.2015.3425
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Abstract

Cyclooxygenase‑2 (COX‑2) has a critical role in the invasiveness and metastasis of gastric cancer. In addition, paclitaxel (PTX) and harmine (HM) were reported to be potential therapeutic drug candidates for cancer therapy; however, the synergistic antitumor effect of PTX and HM combined treatment on the human gastric cancer cells remains to be elucidated. The aim of the present study was to evaluate the effects of PTX and/or HM on the cell migration and invasion in two human gastric cancer cell lines, SGC‑7901 and MKN‑45. MTT assay was used to detect the growth inhibition induced by PTX and HM . The Transwell assay was employed to assess the effects of PTX and HM on the cell migration and invasion. The expression levels of COX-2 and matrix metalloproteinase-9 (MMP-9) were analyzed by western blot analysis. The results demonstrated that PTX and HM inhibited cell proliferation in a dose‑dependent manner. Individually PTX and HM were able to inhibit the migration and invasion of two human gastric cancer cells; however, the combination of PTX and HM exerted synergistic effects on migration and invasion inhibition, with downregulation of COX‑2 and matrix metalloproteinase (MMP)‑9. In conclusion, the results of the present study indicated that combination chemotherapy using PTX with HM exerted an antitumor effect, which may be implicated for the treatment of gastric cancer. Of note, the combination of the two drugs inhibited migration and invasion more effectively compared with each drug alone, the mechanism of which proceeded via the downregulation of COX‑2 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Sun K, Tang XH and Xie YK: Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase‑2 expression. Oncol Lett 10: 1649-1654, 2015.
APA
Sun, K., Tang, X., & Xie, Y. (2015). Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase‑2 expression. Oncology Letters, 10, 1649-1654. https://doi.org/10.3892/ol.2015.3425
MLA
Sun, K., Tang, X., Xie, Y."Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase‑2 expression". Oncology Letters 10.3 (2015): 1649-1654.
Chicago
Sun, K., Tang, X., Xie, Y."Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase‑2 expression". Oncology Letters 10, no. 3 (2015): 1649-1654. https://doi.org/10.3892/ol.2015.3425
Copy and paste a formatted citation
x
Spandidos Publications style
Sun K, Tang XH and Xie YK: Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase‑2 expression. Oncol Lett 10: 1649-1654, 2015.
APA
Sun, K., Tang, X., & Xie, Y. (2015). Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase‑2 expression. Oncology Letters, 10, 1649-1654. https://doi.org/10.3892/ol.2015.3425
MLA
Sun, K., Tang, X., Xie, Y."Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase‑2 expression". Oncology Letters 10.3 (2015): 1649-1654.
Chicago
Sun, K., Tang, X., Xie, Y."Paclitaxel combined with harmine inhibits the migration and invasion of gastric cancer cells through downregulation of cyclooxygenase‑2 expression". Oncology Letters 10, no. 3 (2015): 1649-1654. https://doi.org/10.3892/ol.2015.3425
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