Analysis of p16 gene mutations and their expression using exhaled breath condensate in non‑small‑cell lung cancer

Chen,Jin‑Liang; Chen,Jian‑Rong; Huang,Fen‑Fen; Tao,Guo‑Hua; Zhou,Feng; Tao,Yi‑Jiang

doi:10.3892/ol.2015.3426

September-2015 Volume 10 Issue 3

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September-2015 Volume 10 Issue 3

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Article

Analysis of p16 gene mutations and their expression using exhaled breath condensate in non‑small‑cell lung cancer

Authors:
- Jin‑Liang Chen
- Jian‑Rong Chen
- Fen‑Fen Huang
- Guo‑Hua Tao
- Feng Zhou
- Yi‑Jiang Tao
View Affiliations / Copyright

Affiliations: Department of Respiratory Medicine, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China, Biochemistry Laboratory, The Second Affiliated Hospital of Nantong University, Nantong, Jiangsu 226001, P.R. China
Pages: 1477-1480
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Published online on: June 25, 2015

https://doi.org/10.3892/ol.2015.3426
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Abstract

The aim of the present study was to investigate the mutational status of exons 1 and 2 of the p16 gene in the exhaled breath condensate (EBC) of patients with non‑small‑cell lung cancer (NSCLC) and determine the feasibility and clinical significance of applying EBC in the diagnosis of NSCLC. polymerase chain reaction and DNA sequencing were applied to detect exon 1 and 2 alterations of the p16 gene in EBC by comparing 58 samples from NSCLC patients and 30 from healthy controls. Of the 58 EBC samples from NSCLC patients, 54 were successfully tested and 8 cases of mutations were identified, of which 3 were in exon 1 and 5 in exon 2. The mutation rate was 14.81% (8/54). There were no p16 gene mutations in the 30 samples obtained from healthy controls. EBC p16 gene mutations exhibited no statistically significant differences according to gender, smoking history, pathological type, degree of differentiation and presence or absence of lymph node metastasis. The p16 gene mutation rate was proportional to the tumor stage (P<0.05). Therefore, the detection of the p16 gene mutation in EBC may be used as a novel molecular marker to assist in the diagnosis of NSCLC.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Analysis of p16 gene mutations and their expression using exhaled breath condensate in non‑small‑cell lung cancer

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Abstract

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