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Article

Silencing of survivin by YM155 induces apoptosis and growth arrest in hepatocellular carcinoma cells

  • Authors:
    • Changhe Zhang
    • Xiaofei Cao
    • Yongxiang Gei
    • Yong Wang
    • Guiyuan Liu
    • Guochang Cheng
    • Qinghong Liu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Taizhou Peoples' Hospital, Taizhou, Jiangsu 225300, P.R. China
  • Pages: 1627-1631
    |
    Published online on: July 2, 2015
       https://doi.org/10.3892/ol.2015.3451
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Abstract

Survivin overactivation is a frequent event in human hepatocellular carcinoma (HCC), due to its function in the induction of hepatocyte proliferation and apoptotic dysfunction. Recently, a novel survivin inhibitor named YM155, has demonstrated broad antitumor effects against various malignant tumors. Therefore, the present study aimed to explore how this agent may impact on HCC and elucidate its underlying mechanism of action. Immunohistochemical analysis was performed on 8 specimens of human HCC, to assess the protein expression of survivin and phosphorylated retinoblastoma tumor suppressor (p‑Rb). In addition, in vitro, HepG2 and Huh7 human HCC cell lines were exposed to 100 µM YM155 for up to 72 h and the cell viability was subsequently determined using MTT assay. Furthermore, the apoptotic status of YM155‑treated HCC cells was investigated by flow cytometry, and the protein levels of survivin, procaspase‑3 and p‑Rb in YM155‑treated HCC cells were assessed by immunoblotting analysis. The results demonstrated that HCC specimens expressed high levels of survivin and p‑Rb protein compared with those of adjacent noncancerous liver tissues. In vitro, YM155 significantly induced HCC cell apoptosis and growth arrest. At the protein level, YM155 markedly inhibited survivin and p‑Rb expression, and elevated procaspase‑3. YM155 demonstrated significant antitumor effects on HCC cells in the present study. These effects were associated with its anti‑proliferative and apoptosis‑induction activities. YM155 requires further investigation as a novel agent for potential use as a therapeutic strategy for the treatment of HCC.
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1 

Guglielmi A, Ruzzenente A, Conci S, et al: Hepatocellular carcinoma: Surgical perspectives beyond the barcelona clinic liver cancer recommendations. World J Gastroenterol. 20:7525–7533. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Zhang Y, Shi ZL, Yang X and Yin ZF: Targeting of circulating hepatocellular carcinoma cells to prevent postoperative recurrence and metastasis. World J Gastroenterol. 20:142–147. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Shindoh J, Kaseb A and Vauthey JN: Surgical strategy for liver cancers in the era of effective chemotherapy. Liver Cancer. 2:47–54. 2013. View Article : Google Scholar : PubMed/NCBI

4 

Zhao X, Ogunwobi OO and Liu C: Survivin inhibition is critical for Bcl-2 inhibitor-induced apoptosis in hepatocellular carcinoma cells. PLoS One. 6:e219802011. View Article : Google Scholar : PubMed/NCBI

5 

Liu JL, Zhang XJ, Zhang Z, Zhang AH, Wang W and Dong JH: Meta-analysis: Prognostic value of survivin in patients with hepatocellular carcinoma. PLoS One. 8:e833502013. View Article : Google Scholar : PubMed/NCBI

6 

Cheung CH, Huang CC, Tsai FY, et al: Survivin - biology and potential as a therapeutic target in oncology. Onco Targets Ther. 6:1453–1462. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Yamanaka K, Nakata M, Kaneko N, et al: YM155, a selective survivin suppressant, inhibits tumor spread and prolongs survival in a spontaneous metastatic model of human triple negative breast cancer. Int J Oncol. 39:569–575. 2011.PubMed/NCBI

8 

Tao YF, Lu J, Du XJ, et al: Survivin selective inhibitor YM155 induce apoptosis in SK-NEP-1 Wilms tumor cells. BMC Cancer. 12:6192012. View Article : Google Scholar : PubMed/NCBI

9 

Lai PC, Chen SH, Yang SH, Cheng CC, Chiu TH and Huang YT: Novel survivin inhibitor YM155 elicits cytotoxicity in glioblastoma cell lines with normal or deficiency DNA-dependent protein kinase activity. Pediatr Neonatol. 53:199–204. 2012. View Article : Google Scholar : PubMed/NCBI

10 

Liang H, Zhang L, Xu R and Ju XL: Silencing of survivin using YM155 induces apoptosis and chemosensitization in neuroblastomas cells. Eur Rev Med Pharmacol Sci. 17:2909–2915. 2013.PubMed/NCBI

11 

Koike H, Nitta T, Sekine Y, et al: YM155 reverses rapamycin resistance in renal cancer by decreasing survivin. J Cancer Res Clin Oncol. 140:1705–1713. 2014. View Article : Google Scholar : PubMed/NCBI

12 

Wang H, Jiang S, Zhang Y, et al: High expression of thymosin beta 10 predicts poor prognosis for hepatocellular carcinoma after hepatectomy. World J Surg Oncol. 12:2262014. View Article : Google Scholar : PubMed/NCBI

13 

Charette N, De Saeger C, Horsmans Y, Leclercq I and Stärkel P: Salirasib sensitizes hepatocarcinoma cells to TRAIL-induced apoptosis through DR5 and survivin-dependent mechanisms. Cell Death Dis. 4:e4712013. View Article : Google Scholar : PubMed/NCBI

14 

Reed CA, Mayhew CN, McClendon AK, Yang X, Witkiewicz A and Knudsen ES: RB has a critical role in mediating the in vivo checkpoint response, mitigating secondary DNA damage and suppressing liver tumorigenesis initiated by aflatoxin B1. Oncogene. 28:4434–4443. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Nojiri S and Joh T: Albumin suppresses human hepatocellular carcinoma proliferation and the cell cycle. Int J Mol Sci. 15:5163–5174. 2014. View Article : Google Scholar : PubMed/NCBI

16 

Kaneko N, Mitsuoka K, Amino N, et al: Combination of YM155, a survivin suppressant, with bendamustine and rituximab: A new combination therapy to treat relapsed/refractory diffuse large B-cell lymphoma. Clin Cancer Res. 20:1814–1822. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Fabregat I: Dysregulation of apoptosis in hepatocellular carcinoma cells. World J Gastroenterol. 15:513–520. 2009. View Article : Google Scholar : PubMed/NCBI

18 

Mobahat M, Narendran A and Riabowol K: Survivin as a preferential target for cancer therapy. Int J Mol Sci. 15:2494–2516. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Coumar MS, Tsai FY, Kanwar JR, Sarvagalla S and Cheung CH: Treat cancers by targeting survivin: Just a dream or future reality? Cancer Treat Rev. 39:802–811. 2013. View Article : Google Scholar : PubMed/NCBI

20 

Rauch A, Hennig D, Schäfer C, et al: Survivin and YM155: How faithful is the liaison? Biochim Biophys Acta. 1845:202–220. 2014.PubMed/NCBI

21 

Zeng J, Sun Y, Wu K, et al: Chemopreventive and chemotherapeutic effects of intravesical silibinin against bladder cancer by acting on mitochondria. Mol Cancer Ther. 10:104–116. 2011. View Article : Google Scholar : PubMed/NCBI

22 

Zhang R, Humphreys I, Sahu RP, Shi Y and Srivastava SK: In vitro and in vivo induction of apoptosis by capsaicin in pancreatic cancer cells is mediated through ROS generation and mitochondrial death pathway. Apoptosis. 13:1465–1478. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Taniguchi H, Horinaka M, Yoshida T, et al: Targeting the glyoxalase pathway enhances TRAIL efficacy in cancer cells by downregulating the expression of antiapoptotic molecules. Mol Cancer Ther. 11:2294–2300. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Sakai T, Eskander RN, Guo Y, et al: Flavokawain B, a kava chalcone, induces apoptosis in synovial sarcoma cell lines. J Orthop Res. 30:1045–1050. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Loor G, Kondapalli J, Iwase H, et al: Mitochondrial oxidant stress triggers cell death in simulated ischemia-reperfusion. Biochim Biophys Acta. 1813:1382–1394. 2011. View Article : Google Scholar : PubMed/NCBI

26 

Baker SJ and Reddy EP: CDK4: A key player in the cell cycle, development and cancer. Genes Cancer. 3:658–669. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Hu H, Li Z, Chen J, et al: P16 reactivation induces anoikis and exhibits anti tumour potency by down regulating Akt/survivin signalling in hepatocellular carcinoma cells. Gut. 60:710–721. 2011. View Article : Google Scholar : PubMed/NCBI

28 

Deng L, Lu Y, Zhao X, et al: Ran GTPase protein promotes human pancreatic cancer proliferation by deregulating the expression of Survivin and cell cycle proteins. Biochem Biophys Res Commun. 440:322–329. 2013. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang C, Cao X, Gei Y, Wang Y, Liu G, Cheng G and Liu Q: Silencing of survivin by YM155 induces apoptosis and growth arrest in hepatocellular carcinoma cells. Oncol Lett 10: 1627-1631, 2015.
APA
Zhang, C., Cao, X., Gei, Y., Wang, Y., Liu, G., Cheng, G., & Liu, Q. (2015). Silencing of survivin by YM155 induces apoptosis and growth arrest in hepatocellular carcinoma cells. Oncology Letters, 10, 1627-1631. https://doi.org/10.3892/ol.2015.3451
MLA
Zhang, C., Cao, X., Gei, Y., Wang, Y., Liu, G., Cheng, G., Liu, Q."Silencing of survivin by YM155 induces apoptosis and growth arrest in hepatocellular carcinoma cells". Oncology Letters 10.3 (2015): 1627-1631.
Chicago
Zhang, C., Cao, X., Gei, Y., Wang, Y., Liu, G., Cheng, G., Liu, Q."Silencing of survivin by YM155 induces apoptosis and growth arrest in hepatocellular carcinoma cells". Oncology Letters 10, no. 3 (2015): 1627-1631. https://doi.org/10.3892/ol.2015.3451
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang C, Cao X, Gei Y, Wang Y, Liu G, Cheng G and Liu Q: Silencing of survivin by YM155 induces apoptosis and growth arrest in hepatocellular carcinoma cells. Oncol Lett 10: 1627-1631, 2015.
APA
Zhang, C., Cao, X., Gei, Y., Wang, Y., Liu, G., Cheng, G., & Liu, Q. (2015). Silencing of survivin by YM155 induces apoptosis and growth arrest in hepatocellular carcinoma cells. Oncology Letters, 10, 1627-1631. https://doi.org/10.3892/ol.2015.3451
MLA
Zhang, C., Cao, X., Gei, Y., Wang, Y., Liu, G., Cheng, G., Liu, Q."Silencing of survivin by YM155 induces apoptosis and growth arrest in hepatocellular carcinoma cells". Oncology Letters 10.3 (2015): 1627-1631.
Chicago
Zhang, C., Cao, X., Gei, Y., Wang, Y., Liu, G., Cheng, G., Liu, Q."Silencing of survivin by YM155 induces apoptosis and growth arrest in hepatocellular carcinoma cells". Oncology Letters 10, no. 3 (2015): 1627-1631. https://doi.org/10.3892/ol.2015.3451
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