Expression and mutation analysis of Cyclin A and Ki‑67 in glioma and their correlation with tumor progression

  • Authors:
    • Cheng Miao
    • Zhiyong Wang
    • Jing Yang
    • Jing Li
    • Xunzhao Gao
  • View Affiliations

  • Published online on: July 8, 2015     https://doi.org/10.3892/ol.2015.3474
  • Pages: 1716-1720
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Abstract

The present study aimed to analyze the expression and mutation of Cyclin A and Ki‑67 in gliomas, and determine their correlation with tumor progression. Tissue samples of 186 diagnosed glioma patients were examined immunohistochemically for Cyclin A and Ki‑67 expression. Gene mutation analysis was performed on genomic DNA extracted from patient samples, using polymerase chain reaction amplification and sequencing. Cyclin A and Ki‑67 expression were observed in the glioma and lymphatic metastasis tissues, and were analyzed using SPSS 14.0 statistical software. Of the total patients, 64 (34.41%) were Cyclin A‑positive and 68 (36.56%) were Ki‑67‑positive. The expression of Cyclin A and Ki‑67 in glioma was positively correlated with lymphatic metastasis. Statistically significant differences were observed in the mutation rate of Ki‑67 (P<0.05), but not Cyclin A (P>0.05), between the gliomas and metastatic tumors. In conclusion, Cyclin A and Ki‑67 are highly expressed in glioma tissues, and their expression and mutation are associated with the lymphatic metastasis of glioma in the brain. It may be concluded that Cyclin A and Ki‑67 may be used as biomarkers to guide the diagnosis of glioma and evaluate the prognosis of affected patients.
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September-2015
Volume 10 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Miao C, Wang Z, Yang J, Li J and Gao X: Expression and mutation analysis of Cyclin A and Ki‑67 in glioma and their correlation with tumor progression. Oncol Lett 10: 1716-1720, 2015
APA
Miao, C., Wang, Z., Yang, J., Li, J., & Gao, X. (2015). Expression and mutation analysis of Cyclin A and Ki‑67 in glioma and their correlation with tumor progression. Oncology Letters, 10, 1716-1720. https://doi.org/10.3892/ol.2015.3474
MLA
Miao, C., Wang, Z., Yang, J., Li, J., Gao, X."Expression and mutation analysis of Cyclin A and Ki‑67 in glioma and their correlation with tumor progression". Oncology Letters 10.3 (2015): 1716-1720.
Chicago
Miao, C., Wang, Z., Yang, J., Li, J., Gao, X."Expression and mutation analysis of Cyclin A and Ki‑67 in glioma and their correlation with tumor progression". Oncology Letters 10, no. 3 (2015): 1716-1720. https://doi.org/10.3892/ol.2015.3474