MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma

Zhang,Jufeng; Wang,Daping; Xiong,Jianyi; Chen,Lei; Huang,Jianghong

doi:10.3892/ol.2015.3503

MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma

Authors:
- Jufeng Zhang
- Daping Wang
- Jianyi Xiong
- Lei Chen
- Jianghong Huang
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Affiliations: Department of Biological Sciences, School of Life Science, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China, Department of Orthopaedics, Shenzhen Second People's Hospital, Shenzhen, Guangdong 518039, P.R. China
Published online on: July 17, 2015 https://doi.org/10.3892/ol.2015.3503
Pages: 2135-2141
Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

A body of evidence has indicated that microRNAs (miRNAs) may have significant roles in cancer. Aberrant expression of miRNAs has frequently been observed in various human malignancies, including osteosarcoma (OS). However, the roles of miRNAs in OS remain poorly understood. In the present study, high‑throughput deep sequencing was performed to screen for deregulated miRNAs in OS. Screening identified 310 miRNAs which were significantly overexpressed and 41 miRNAs which were significantly downregulated (>2‑fold) in OS samples, compared with adjacent non‑tumor bone tissues. Among these miRNAs, miR‑33a‑5p was notably downregulated. TaqMan reverse transcription-polymerase chain reaction analysis further verified that miR‑33a‑5p expression was significantly reduced in a large cohort of human OS samples. Enhancing miR‑33a‑5p expression via transfection with miR-33a-5p precursor significantly inhibited OS cell growth, suggesting potential antitumor properties of miR‑33a‑5p. The results of the present study provide novel insights into the miRNAs involved in OS, and suggest that miR‑33a‑5p may function as a tumor suppressor in OS. Therefore, miR‑33a‑5p may be able to serve as a diagnostic and therapeutic target for OS treatment.

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