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MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma

  • Authors:
    • Jufeng Zhang
    • Daping Wang
    • Jianyi Xiong
    • Lei Chen
    • Jianghong Huang
  • View Affiliations / Copyright

    Affiliations: Department of Biological Sciences, School of Life Science, Guangdong Pharmaceutical University, Guangzhou, Guangdong 510006, P.R. China, Department of Orthopaedics, Shenzhen Second People's Hospital, Shenzhen, Guangdong 518039, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2135-2141
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    Published online on: July 17, 2015
       https://doi.org/10.3892/ol.2015.3503
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Abstract

A body of evidence has indicated that microRNAs (miRNAs) may have significant roles in cancer. Aberrant expression of miRNAs has frequently been observed in various human malignancies, including osteosarcoma (OS). However, the roles of miRNAs in OS remain poorly understood. In the present study, high‑throughput deep sequencing was performed to screen for deregulated miRNAs in OS. Screening identified 310 miRNAs which were significantly overexpressed and 41 miRNAs which were significantly downregulated (>2‑fold) in OS samples, compared with adjacent non‑tumor bone tissues. Among these miRNAs, miR‑33a‑5p was notably downregulated. TaqMan reverse transcription-polymerase chain reaction analysis further verified that miR‑33a‑5p expression was significantly reduced in a large cohort of human OS samples. Enhancing miR‑33a‑5p expression via transfection with miR-33a-5p precursor significantly inhibited OS cell growth, suggesting potential antitumor properties of miR‑33a‑5p. The results of the present study provide novel insights into the miRNAs involved in OS, and suggest that miR‑33a‑5p may function as a tumor suppressor in OS. Therefore, miR‑33a‑5p may be able to serve as a diagnostic and therapeutic target for OS treatment.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang J, Wang D, Xiong J, Chen L and Huang J: MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma. Oncol Lett 10: 2135-2141, 2015.
APA
Zhang, J., Wang, D., Xiong, J., Chen, L., & Huang, J. (2015). MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma. Oncology Letters, 10, 2135-2141. https://doi.org/10.3892/ol.2015.3503
MLA
Zhang, J., Wang, D., Xiong, J., Chen, L., Huang, J."MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma". Oncology Letters 10.4 (2015): 2135-2141.
Chicago
Zhang, J., Wang, D., Xiong, J., Chen, L., Huang, J."MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma". Oncology Letters 10, no. 4 (2015): 2135-2141. https://doi.org/10.3892/ol.2015.3503
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang J, Wang D, Xiong J, Chen L and Huang J: MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma. Oncol Lett 10: 2135-2141, 2015.
APA
Zhang, J., Wang, D., Xiong, J., Chen, L., & Huang, J. (2015). MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma. Oncology Letters, 10, 2135-2141. https://doi.org/10.3892/ol.2015.3503
MLA
Zhang, J., Wang, D., Xiong, J., Chen, L., Huang, J."MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma". Oncology Letters 10.4 (2015): 2135-2141.
Chicago
Zhang, J., Wang, D., Xiong, J., Chen, L., Huang, J."MicroRNA-33a-5p suppresses growth of osteosarcoma cells and is downregulated in human osteosarcoma". Oncology Letters 10, no. 4 (2015): 2135-2141. https://doi.org/10.3892/ol.2015.3503
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