Imaging of human pancreatic cancer xenografts by single-photon emission computed tomography with 99mTc-Hynic-PEG-AE105

  • Authors:
    • Xin Zhang
    • Ye Tian
    • Fangfang Sun
    • Hongbo Feng
    • Chun Yang
    • Xiaoyan Gong
    • Guang Tan
  • View Affiliations

  • Published online on: July 17, 2015     https://doi.org/10.3892/ol.2015.3504
  • Pages: 2253-2258
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Abstract

The elevated expression of urokinase-type plasminogen activator receptor (uPAR) is associated with the poor prognosis of pancreatic cancer patients. Thus, uPAR is a promising candidate as a molecular target for the non‑invasive imaging of pancreatic cancer. The present study aimed to develop a technetium-99m (99mTc)‑labeled uPAR‑binding peptide for non‑invasive single-photon emission computed tomography (SPECT) assessment of uPAR expression in pancreatic cancer xenograft models. A linear high‑affinity uPAR peptide antagonist, Hynic‑PEG‑AE105, was labeled with 99mTc. Human uPAR‑positive pancreatic cancer BxPC‑3 cells were inoculated into nude mice. SPECT was performed in the pancreatic cancer xenograft mice models. The results showed that the rate of the 99mTc labeling of Hynic‑PEG‑AE105 was 97.72±1.73%. The tumor uptake of 99mTc‑Hynic‑PEG‑AE105 was higher than the control inactive peptide 99mTc‑Hynic‑PEG‑AE105mut at 4 h (3.37±0.11 vs. 1.36±0.18; P<0.001) and 6 h (3.64±0.25 vs. 1.28±0.20; P<0.001) (n=10). Moreover, a significant correlation was observed between the tumor uptake of 99mTc‑Hynic‑PEG‑AE105 and uPAR expression (r=0.791, P=0.006). In conclusion, in the present study, a peptide‑based SPECT tracer, 99mTc‑Hynic‑PEG‑AE105, with a high purity and specific radioactivity was synthesized. 99mTc‑Hynic‑PEG‑AE105 is a promising agent for the non-invasive determination of uPAR expression in pancreatic cancer.
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October-2015
Volume 10 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Zhang X, Tian Y, Sun F, Feng H, Yang C, Gong X and Tan G: Imaging of human pancreatic cancer xenografts by single-photon emission computed tomography with 99mTc-Hynic-PEG-AE105. Oncol Lett 10: 2253-2258, 2015
APA
Zhang, X., Tian, Y., Sun, F., Feng, H., Yang, C., Gong, X., & Tan, G. (2015). Imaging of human pancreatic cancer xenografts by single-photon emission computed tomography with 99mTc-Hynic-PEG-AE105. Oncology Letters, 10, 2253-2258. https://doi.org/10.3892/ol.2015.3504
MLA
Zhang, X., Tian, Y., Sun, F., Feng, H., Yang, C., Gong, X., Tan, G."Imaging of human pancreatic cancer xenografts by single-photon emission computed tomography with 99mTc-Hynic-PEG-AE105". Oncology Letters 10.4 (2015): 2253-2258.
Chicago
Zhang, X., Tian, Y., Sun, F., Feng, H., Yang, C., Gong, X., Tan, G."Imaging of human pancreatic cancer xenografts by single-photon emission computed tomography with 99mTc-Hynic-PEG-AE105". Oncology Letters 10, no. 4 (2015): 2253-2258. https://doi.org/10.3892/ol.2015.3504