Potentiation of the antitumor activity of adriamycin against osteosarcoma by cannabinoid WIN-55,212-2

  • Authors:
    • Feng Niu
    • Song Zhao
    • Chang‑Yan Xu
    • Hui Sha
    • Gui‑Bin Bi
    • Lin Chen
    • Long Ye
    • Ping Gong
    • Tian‑Hong Nie
  • View Affiliations

  • Published online on: July 23, 2015     https://doi.org/10.3892/ol.2015.3525
  • Pages: 2415-2421
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Abstract

Osteosarcoma is the most frequent primary malignant bone tumor that occurs in children and adolescents. The present study aimed to identify novel therapeutic strategies for osteosarcoma, by assessing the antitumor activity of the cannabinoid WIN‑55,212‑2 and its combined effect with adriamycin (ADM) against the MG‑63 human osteosarcoma cell line. To evaluate the antiproliferative action of these molecules, a Cell Counting kit‑8 (CCK‑8) assay was used. The ability of cannabinoid to inhibit the migration, invasion and angiogenic activity of MG‑63 cells were assessed by scratch, Transwell® chamber and angiogenesis assays, respectively, in vitro. To examine the alterations in expression of targeted genes, quantitative polymerase chain reaction and western blot analysis were used. The administration of cannabinoid combined with ADM was demonstrated to inhibit the growth of MG‑63 cells, resulting in a cell viability of 32.12±3.13%, which was significantly lower (P<0.05) compared with the cell viability following treatment with cannabinoid (70.86±7.55%) and ADM (62.87±5.98%) alone. Greater antimetastasis and antiangiogenic activities were also observed following the coadministration of the two agents compared with individual treatments and controls. In addition, the expression levels of Notch‑1, matrix metalloproteinase‑2 (MMP‑2) and vascular endothelial growth factor (VEGF) in MG‑63 cells were downregulated following the treatments with cannabinoid alone or in combination with ADM. In conclusion, the present findings demonstrated that cannabinoid WIN‑55,212‑2 may significantly potentiate the antiproliferative, antimetastasis and antiangiogenic effects of ADM against MG‑63 cells via the downregulation of Notch‑1, MMP‑2 and VEGF. These findings may offer a novel strategy for the treatment of osteosarcoma.
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October-2015
Volume 10 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Niu F, Zhao S, Xu CY, Sha H, Bi GB, Chen L, Ye L, Gong P and Nie TH: Potentiation of the antitumor activity of adriamycin against osteosarcoma by cannabinoid WIN-55,212-2. Oncol Lett 10: 2415-2421, 2015
APA
Niu, F., Zhao, S., Xu, C., Sha, H., Bi, G., Chen, L. ... Nie, T. (2015). Potentiation of the antitumor activity of adriamycin against osteosarcoma by cannabinoid WIN-55,212-2. Oncology Letters, 10, 2415-2421. https://doi.org/10.3892/ol.2015.3525
MLA
Niu, F., Zhao, S., Xu, C., Sha, H., Bi, G., Chen, L., Ye, L., Gong, P., Nie, T."Potentiation of the antitumor activity of adriamycin against osteosarcoma by cannabinoid WIN-55,212-2". Oncology Letters 10.4 (2015): 2415-2421.
Chicago
Niu, F., Zhao, S., Xu, C., Sha, H., Bi, G., Chen, L., Ye, L., Gong, P., Nie, T."Potentiation of the antitumor activity of adriamycin against osteosarcoma by cannabinoid WIN-55,212-2". Oncology Letters 10, no. 4 (2015): 2415-2421. https://doi.org/10.3892/ol.2015.3525