Germline and somatic mutations of the APC gene in papillary thyroid carcinoma associated with familial adenomatous polyposis: Analysis of three cases and a review of the literature

  • Authors:
    • Kensuke Kumamoto
    • Hideyuki Ishida
    • Tomonori Ohsawa
    • Keiichiro Ishibashi
    • Mineko Ushiama
    • Teruhiko Yoshida
    • Takeo Iwama
  • View Affiliations

  • Published online on: August 6, 2015     https://doi.org/10.3892/ol.2015.3578
  • Pages: 2239-2243
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Abstract

Patients with familial adenomatous polyposis (FAP), which is caused by the dysfunction of the adenomatous polyposis coli (APC) protein, have the possibility of developing extracolonic manifestations, including thyroid cancer (TC), congenital hypertrophy of the retinal pigment epithelium, desmoid tumors, and gastric and duodenal adenomas. The pathogenesis of these disorders associated with FAP is considered to be affected by the site of the germline mutation on the APC gene as a genotype‑phenotype correlation. Moreover, β‑catenin binding sites consist of 20‑amino acid repeats (20‑AARs) in the APC protein, and they are essential for the development of colorectal adenomas and certain other extracolonic manifestations. The present study retrospectively analyzed the germline and somatic mutations of the APC gene in three papillary TC patients with FAP to analyze the association between the remaining number of 20‑AARs and the development of TC. The mutation sites of two TCs did not include 20‑AARs in each allele. In one patient, the remaining number of 20‑AARs was two in the germline mutation and zero in the somatic mutation. Together with the data on 13 FAP‑associated thyroid cancerous lesions in 3 FAP patients reported previously, the majority of the remaining numbers of 20‑AARs was zero in the TC patients with FAP (13/16; 81.3%). Consequently, the APC/β‑catenin signaling pathway may be strongly involved with the pathogenesis of TC with FAP. Further accumulation of FAP patients with TC will be required to confirm the molecular pathogenesis of TC.

Introduction

Thyroid cancer (TC) in a patient with familial adenomatous polyposis (FAP) was first described in 1949 by Crail (1). The association between FAP and TC was subsequently reported in 1968 (2). Approximately 1–2% of FAP patients develop TC within their lifetime (3,4). A previous study from Japan (3) estimated the risk of TC in women with FAP to be ~23-fold higher than that of women without FAP, while the risk of developing TC in young women under the age of 35 years old with FAP has been estimated to be ~160 times that of normal individuals according to the St Mark's Hospital Polyposis Registry (5).

It is well known that dysfunction of the adenomatous poliposis coli (APC) protein due to germline mutations causes development of hundreds and thousands of colorectal polyps, a number of which may progress to colorectal cancer for the majority of patients, unless a prophylactic colectomy is performed. The clinical phenotype, including profuse and attenuated types, appears to be associated with the site of germline mutations on the APC gene (6,7). Moreover, the pathogenesis of extracolonic manifestations, including congenital hypertrophy of the retinal pigment epithelium, desmoid tumors, and gastric and duodenal adenomas, is considered to be associated with the site of the germline mutation on the APC gene. This association is termed the ‘Genotype-Phenotype Correlation’ (6,7). However, a somatic mutation that occurs on the other allele may affect the clinical features of FAP patients. The somatic mutation, termed the ‘second hit’ (8), is usually determined by the site of the germline mutation. This mechanism is widely accepted as the ‘just right’ model (9) and the ‘loose fit’ model (10). Normal APC functions include the degradation of β-catenin following the binding to the APC protein at one of the 7 sites, consisting of 20-amino acid repeats (20-AARs), between codons 1265 and 2034 (11). The remaining number of 20-AAR sites on each allele is counted through the sites of the germline mutation and somatic mutation. A previous study (10,12) demonstrated that the remaining number of 20-AARs in cases of colorectal adenomas range between one and three. The remaining two or three 20-AARs on each allele is associated with gastric and duodenal adenomas, and desmoid tumors (12). Little is known with regard to this in TC patients with FAP. The present study aimed to investigate the remaining number of 20-AARs in TC developed in FAP patients. The study reports the cases of three TC patients with FAP who underwent a resection of the TC in Saitama Medical Center (Saitama, Japan) to investigate the association between the remaining number of 20-AARs by analyzing the germline and somatic mutations on the APC gene. The study then discusses the results together with previously published data.

Patients and methods

Ethics and consent

This study was performed in accordance with ethical guidelines for clinical research with the approval of the institutional ethics committee of Saitama Medical Center, Saitama Medical University. Based upon a presumptive clinical diagnosis of FAP, patients received genetic counseling at the Department of Digestive Tract and General Surgery in Saitama Medical Center, and written informed consent for further genetic analyses was obtained from the individuals included in the study.

Patients

Genetic analysis of the following three patients with FAP-associated TC was performed.

Patient 1

The patient was a 25-year-old female who had been diagnosed with FAP at 20 years old. At this time, a prophylactic colectomy had been performed. A thyroid tumor, 10 mm in diameter, was detected in the right lobe in September 2005. The resected specimen was pathologically diagnosed as cribriform-morula variant of papillary thyroid carcinoma (CMPTC).

Patient 2

A 32-year-old female, whose mother and younger sister had been treated for FAP, was diagnosed with FAP plus early colon cancer and a thyroid tumor, 10 mm in diameter, in the left lobe in April 2008. The patient underwent a total colectomy following the diagnosis of sigmoid cancer. A subtotal thyroidectomy was performed two months after the total colectomy. The pathological diagnosis was of CMPTC.

Patient 3

In February 2008, a 38-year-old female underwent a total colectomy and ileorectal anastomosis for stage IV transverse colon cancer following a complete response to oxaliplatin-based (mFOLFOX6) chemotherapy for multiple liver metastases. The mFOLFOX6 regimen comprised of intravenous infusions of oxaliplatin (85 mg/m2) and leucovorin (200 mg/m2) for 2 h, followed by rapid intravenous bolus infusions of 5-fluorouracil (5-FU; 400 mg/m2) for 5 min and continuous intravenous infusion of 5-FU (2,400 mg/m2) for 46 h. This regimen was repeated twelve times every 2 or 3 weeks. Two years later, the patient underwent a total thyroidectomy for thyroid tumors that were 10 mm, 8 mm and 3 mm in diameter. A pathological examination revealed CMPTC. The patient has remained free from colorectal and thyroid cancer recurrence for 36 months since the thyroidectomy.

Germline mutation analysis

For analysis of the germline mutations, peripheral blood samples were collected from each patient. Genomic DNA extraction was performed using a QIAamp DNA Blood Mini kit (Qiagen, Hilden, Germany). A small piece of thyroid cancer tissue and corresponding normal tissue were taken from the surgically resected specimens to identify any germline and somatic mutations. Part of the genetic analysis of these samples was commissioned to Falco Biosystems Ltd., (Kyoto, Japan). Exon-by-exon polymerase chain reaction (PCR) of the 1st-15th APC exons and their boundary intronic sequences was performed on ~600 ng DNA using AmpliTaq Gold DNA polymerase (Applied Biosystems Life Technologies, Foster City, CA, USA). The PCR conditions were as follows: Initial denaturation at 94°C for 10 min, followed by 35 cycles of 94°C for 20 sec, 62°C for 30 sec and 72°C 1 min, with a final extention of 72°C for 1 min. PCR products were directly sequenced using BigDye Terminator Ready Reaction Mix (version 1.1; Applied Biosystems Life Technologies) and an ABI 3100 Genetic Analyzer with SeqScape Software (version 2.6; Applied Biosystems Life Technologies).

Results

The results of the genetic analysis on the germline and somatic mutations for the three patients are summarized in Table I.

Table I.

Analysis of association between germline/somatic mutation sites and the remaining number of 20 amino acid repeats in reported cases.

Table I.

Analysis of association between germline/somatic mutation sites and the remaining number of 20 amino acid repeats in reported cases.

Germline mutationSomatic mutation


Patient no.Cancerous lesion no.Mutation typeRemaining numbers of 20-AARsMutation typeRemaining numbers of 20-AARsFirst author, year (ref.)
1  1Codon 917 T deletion0Codon 728 AAT-AT; A deletion0Present study
2  2Codon 1179–11817 bp deletion0Codon 607 T deletion0
3  3Codon 1483 C deletion2Codon 295 G deletion0
4  4Codon 554 CGA→TGA0Codon 308 deletion C0Uchino et al, 2006 (14)
  5 Codon 534 1602 deletion A0
  6 Codon 607 1821 deletion T0
  7 Codon 640 1920 deletion G0
  8 Codon 902 2706 deletion 200
  9 Codon 935 2804 insertion A0
510Codon 175 C deletion0Codon 886, CAG→TAG0Miyaki et al, 2000 (13)
11 LOH0
12 Codon 1536 GAA→TAA3
13 LOH0
14 Codon 1554–1556 A insertion3
615Codon 1110 TCA→TGA0Codon 857 GGA→TGA0
16 Codon 1060–1063 AAAAC deletion0

[i] 20-AARs, 20-amino acid repeats; LOH, loss of heterozygosity.

In patient 1, the genetic analysis of the APC gene from a blood sample and the thyroid cancer tissue identified a germ-line mutation (T deletion at codon 917) and a somatic mutation (A deletion at codon 728), which were each considered to form stop codons, resulting in truncated products of the APC gene. These results suggested that none of the 20-AARs were remaining on the APC gene in the TC cells.

The germline mutation of patient 2, which was analyzed using peripheral blood collected from the patient, was a 7-bp deletion (TATAGTTTA→TA) at codons 1179–1181. The somatic mutation was a deletion of T at codon 607. These results indicate that there is no 20-AAR in the TC cells by the germline and somatic mutations on the APC gene.

In patient 3, the genetic analysis of the APC gene from the blood sample and the thyroid cancer tissue identified a germline mutation (C deletion at codon 1483) and a somatic mutation (G deletion at codon 295), which were each considered to form stop codons, resulting in truncated products of the APC genes. These results suggest that two 20-AARs (two 20-AARs on the germline mutation and zero 20-AARs on the somatic mutation) existed on the APC gene in the TC cells of the patient.

Discussion

The remaining number of 20-AARs following germline and somatic mutations on the APC gene were analyzed in the TC cells developed in the patients with FAP. As shown in Table I, the remaining number of 20-AARs following germline and somatic mutations was zero in two cases (patients 1 and 2), while two 20-AARs remained in one case (patient 3) due to the site of the germline mutation. Mutation analyses of three FAP cases (patients 4–6) with a total of 13 TC lesions have previously been reported in Japan (13,14) and are listed in Table I. Of the 13 cancerous lesions, the remaining number of 20-AARs was zero in 11 lesions. Together with the present cases, the distribution of the number of 20-AARs retained in 16 thyroid cancerous lesions is summarized in Table II. These results indicated that the majority of the remaining number of 20-AARs was zero in the TC patients with FAP (13/16; 81.3%).

Table II.

Analysis of association between the number of 20-AARs retained and TC (n=16).

Table II.

Analysis of association between the number of 20-AARs retained and TC (n=16).

Number of 20-AARs retained in germline/somatic mutations

Cancer type0/00/1 or 1/00/2 or 2/00/3 or 3/01/2 or 2/11/3 or 3/12/3 or 3/2
TC13012000

[i] TC, thyroid cancer; 20-AARs, 20-amino acid repeats.

The location of the APC mutation may dictate the phenotype of FAP. Genotype-phenotype correlation with FAP refers to the correlation between the germline mutation on the APC gene and the clinical phenotype, including extracolonic manifestations (6,7). With regard to the extracolonic manifestations of FAP, the majority of the patients with TC showed APC germline mutations at the 5′ to the mutation cluster region (codons 1286–1513) (15); when two groups of patients were created, FAP patients with TC and without TC, and their germline mutations prior to and following codon 1220 were assessed, significantly more patients with TC exhibited the germline mutation prior to 1220 (15). A recent study (16) demonstrated that APC mutations at codon 1061 and those proximal to codon 512 increased the risk for TC in individuals with FAP. According to the aforementioned data, the sites of the majority of the germline mutations in FAP-associated TC are located proximal to codon 1265, from which 20-AARs begin, resulting in the lack of the 20-AARs in the germline-mutated APC protein. Although somatic mutations of the APC gene for FAP-associated TC have not been fully analyzed in comparison with their germline mutations, the results of the present study and previous reports have demonstrated that the majority of the somatic and germline mutations of APC in FAP patients with TC lead to the total absence of 20-AARs in the TC cells, while few patients have three or less 20-AARs.

The current results showing that the remaining number of 20-AARs in FAP-associated TC was nearly zero were completely different from those for colorectal adenomas and other extracolonic manifestations, the majority of which retain two or three 20-AARs (10,12). In the analysis of desmoid tumors derived from FAP patients, the majority of desmoid cases retained two 20-AARs in the germline mutation, while the majority of desmoids with two APC hits had one somatically-mutated allele with no 20-AARs (12,17). The current consensus is that 20-AARs are associated with the binding to β-catenin through the Wnt signaling pathway (9). When APC that is associated with the Wnt signaling pathway functions normally in endothelial cells, β-catenin is constantly degraded by proteasome following the binding to 20-AARs on the APC protein, leading to the suppression of the expression of genes associated with development and carcinogenesis. The remaining number of 20-AARs is associated with the regulation of the amount of β-catenin. A ‘just right’ model has been proposed to predict the pattern of the second hit following the first hit, including those retaining a few 20-AARs (9).

It appears that the presence of a certain number of 20-AARs is essential for the development of colorectal adenomas and certain other extracolonic manifestations, although two or less 20-AARs have been suggested to be defective with regard to the β-catenin degradation. Based on that mechanism, we suggest that the APC/β-catenin signaling pathway, via the binding to 20-AARs, is strongly involved with the development of TC in patients with FAP, since there were few 20-AARs remaining following the germline and somatic mutations in the present and previous studies.

It has been reported that somatic mutations or loss of heterozygosity of the APC gene are extremely rare in FAP-associated TC (18). Previous studies (1921) have described that the APC mutation is extremely rare in sporadic TC. Regarding sporadic colorectal cancer, 60% of all patients have somatic mutations of the APC gene (22), leading to the development of carcinogenesis.

Frequent absence of the second-hit somatic APC mutation may suggest that the Wnt/β-catenin pathway does not have a major role in the pathogenesis of not only sporadic TC, but also TC associated with FAP, unlike colorectal cancer. Although the germline and somatic APC mutations may be associated with the genetic susceptibility to thyroid carcinogenesis, the alternative pathogenesis may be important for the development of TC with FAP. Previously, several studies demonstrated that RET/PTC rearrangements were frequently activated in FAP-associated TC (23,24). In fact, this hypothesis would be based on the pathogenesis of sporadic TC (25). Furthermore, somatic BRAFV600E mutations were shown to frequently occur in sporadic TC (25). These representative genetic mutations in sporadic TC could cause the pathogenesis of FAP-associated TC, although it will not be a specific mechanism of the onset of FAP-associated TC.

With regard to other factors associated with the pathogenesis of FAP-associated TC, it is particularly worthy of note that the epidemiological findings suggest that FAP-associated TC is a female-dominated manifestation in FAP patients. We propose that sex hormones, such as estrogen and progesterone, may also be important in the development of FAP-associated TC.

In the present study, it was found that the remaining number of 20-AARs on the APC gene by the germline and somatic mutations was zero in the majority of the FAP-associated TC cells, indicating that the Wnt signaling pathway is dominantly involved with the pathogenesis of TC in patients with FAP. Our future studies will clarify the molecular mechanism behind the pathogenesis of FAP-associated TC, including the involvement of RAS, BRAF and RET/PTC, once more FAP-associated TC samples have been collected.

References

1 

Crail HW: Multiple primary malignancies arising in the rectum, brain and thyroid; report of a case. U S Nav Med Bull. 49:123–128. 1949.PubMed/NCBI

2 

Camiel MR, Mulé JE, Alexander LL and Benninghoff DL: Association of thyroid carcinoma with Gardner's syndrome in siblings. N Engl J Med. 278:1056–1058. 1968. View Article : Google Scholar : PubMed/NCBI

3 

Iwama T, Mishima Y and Utsunomiya J: The impact of familial adenomatous polyposis on the tumorigenesis and mortality at the several organs. Its rational treatment. Ann Surg. 217:101–108. 1993. View Article : Google Scholar : PubMed/NCBI

4 

Giardiello FM, Offerhaus GJ, Lee DH, Krush AJ, Tersmette AC, Booker SV, Kelley NC and Hamilton SR: Increased risk of thyroid and pancreatic carcinoma in familial adenomatous polyposis. Gut. 34:1394–1396. 1993. View Article : Google Scholar : PubMed/NCBI

5 

Plail RO, Bussey HJ, Glazer G and Thomson JP: Adenomatous polyposis: An association with carcinoma of the thyroid. Br J Surg. 74:377–380. 1987. View Article : Google Scholar : PubMed/NCBI

6 

Groen EJ, Roos A, Muntinghe FL, Enting RH, de Vries J, Kleibeuker JH, Witjes MJ, Links TP and van Beek AP: Extra-intestinal manifestations of familial adenomatous polyposis. Ann Surg Oncol. 15:2439–2450. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Bertario L, Russo A, Sala P, Varesco L, Giarola M, Mondini P, Pierotti M, Spinelli P and Radice P: Hereditary colorectal tumor registry: Multiple approach to the exploration of genotype-phenotype correlations in familial adenomatous polyposis. J Clin Oncol. 21:1698–1707. 2003. View Article : Google Scholar : PubMed/NCBI

8 

Lamlum H, Ilyas M, Rowan A, Clark S, Johnson V, Bell J, Frayling I, Efstathiou J, Pack K, Payne S, et al: The type of somatic mutation at APC in familial adenomatous polyposis is determined by the site of the germline mutation: A new facet to Knudson's ‘two-hit’ hypothesis. Nat Med. 5:1071–1075. 1999. View Article : Google Scholar : PubMed/NCBI

9 

Albuquerque C, Breukel C, van der Luijt R, Fidalgo P, Lage P, Slors FJ, Leitão CN, Fodde R and Smits R: The ‘just-right’ signaling model: APC somatic mutations are selected based on a specific level of activation of the beta-catenin signaling cascade. Hum Mol Genet. 11:1549–1560. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Crabtree M, Sieber OM, Lipton L, Hodgson SV, Lamlum H, Thomas HJ, Neale K, Phillips RK, Heinimann K and Tomlinson IP: Refining the relation between ‘first hits’ and ‘second hits’ at the APC locus: The ‘loose fit’ model and evidence for differences in somatic mutation spectra among patients. Oncogene. 22:4257–4265. 2003. View Article : Google Scholar : PubMed/NCBI

11 

Rubinfeld B, Albert I, Porfiri E, Munemitsu S and Polakis P: Loss of beta-catenin regulation by the APC tumor suppressor protein correlates with loss of structure due to common somatic mutations of the gene. Cancer Res. 57:4624–4630. 1997.PubMed/NCBI

12 

Miyaki M, Yamaguchi T, Iijima T, Takahashi K, Matsumoto H, Yasutome M, Funata N and Mori T: Difference in characteristics of APC mutations between colonic and extracolonic tumors of FAP patients: Variations with phenotype. Int J Cancer. 122:2491–2497. 2008. View Article : Google Scholar : PubMed/NCBI

13 

Miyaki M, Iijima T, Ishii R, Hishima T, Mori T, Yoshinaga K, Takami H, Kuroki T and Iwama T: Molecular evidence for multicentric development of thyroid carcinomas in patients with familial adenomatous polyposis. Am J Pathol. 157:1825–1827. 2000. View Article : Google Scholar : PubMed/NCBI

14 

Uchino S, Noguchi S, Yamashita H, Yamashita H, Watanabe S, Ogawa T, Tsuno A, Murakami A and Miyauchi A: Mutational analysis of the APC gene in cribriform-morula variant of papillary thyroid carcinoma. World J Surg. 30:775–779. 2006. View Article : Google Scholar : PubMed/NCBI

15 

Cetta F, Montalto G, Gori M, Curia MC, Cama A and Olschwang S: Germline mutations of the APC gene in patients with familial adenomatous polyposis-associated thyroid carcinoma: Results from a European cooperative study. J Clin Endocrinol Metab. 85:286–292. 2000. View Article : Google Scholar : PubMed/NCBI

16 

Septer S, Slowik V, Morgan R, Dai H and Attard T: Thyroid cancer complicating familial adenomatous polyposis: Mutation spectrum of at-risk individuals. Hered Cancer Clin Pract. 11:132013. View Article : Google Scholar : PubMed/NCBI

17 

Latchford A, Volikos E, Johnson V, Rogers P, Suraweera N, Tomlinson I, Phillips R and Silver A: APC mutations in FAP-associated desmoid tumours are non-random but not ‘just right’. Hum Mol Genet. 16:78–82. 2007. View Article : Google Scholar : PubMed/NCBI

18 

Cetta F, Dhamo A, Malagnino G and Barellini L: Germ-line and somatic mutations of the APC gene and/or β-catenin gene in the occurrence of FAP associated thyroid carcinoma. World J Surg. 31:1366–1367. 2007. View Article : Google Scholar : PubMed/NCBI

19 

Curtis L, Wyllie AH, Shaw JJ, Williams GT, Radulescu A, DeMicco C, Haugen DR, Varhaug JE, Lillehaug JR and Wynford-Thomas D: Evidence against involvement of APC mutation in papillary thyroid carcinoma. Eur J Cancer 30A. 984–987. 1994. View Article : Google Scholar

20 

Colletta G, Sciacchitano S, Palmirotta R, Ranieri A, Zanella E, Cama A, Mariani Costantini R, Battista P and Pontecorvi A: Analysis of adenomatous polyposis coli gene in thyroid tumours. Br J Cancer. 70:1085–1088. 1994. View Article : Google Scholar : PubMed/NCBI

21 

Zeki K, Spambalg D, Sharifi N, Gonsky R and Fagin JA: Mutations of the adenomatous polyposis coli gene in sporadic thyroid neoplasms. J Clin Endocrinol Metab. 79:1317–1321. 1994. View Article : Google Scholar : PubMed/NCBI

22 

Powell SM, Zilz N, BeazerBarclay Y, Bryan TM, Hamilton SR, Thibodeau SN, Vogelstein B and Kinzler KW: APC mutations occur early during colorectal tumorigenesis. Nature. 359:235–237. 1992. View Article : Google Scholar : PubMed/NCBI

23 

Cetta F, Chiappetta G, Melillo RM, Petracci M, Montalto G, Santoro M and Fusco A: The ret/ptc1 oncogene is activated in familial adenomatous polyposis-associated thyroid papillary carcinomas. J Clin Endocrinol Metab. 83:1003–1006. 1998. View Article : Google Scholar : PubMed/NCBI

24 

Soravia C, Sugg SL, Berk T, Mitri A, Cheng H, Gallinger S, Cohen Z, Asa SL and Bapat BV: Familial adenomatous polyposis-associated thyroid cancer: A clinical, pathological and molecular genetics study. Am J Pathol. 154:127–135. 1999. View Article : Google Scholar : PubMed/NCBI

25 

Romei C, Fugazzola L, Puxeddu E, Frasca F, Viola D, Muzza M, Moretti S, Nicolosi ML, Giani C, Cirello V, et al: Modifications in the papillary thyroid cancer gene profile over the last 15 years. J Clin Endocrinol Metab. 97:E1758–E1765. 2012. View Article : Google Scholar : PubMed/NCBI

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October-2015
Volume 10 Issue 4

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Spandidos Publications style
Kumamoto K, Ishida H, Ohsawa T, Ishibashi K, Ushiama M, Yoshida T and Iwama T: Germline and somatic mutations of the APC gene in papillary thyroid carcinoma associated with familial adenomatous polyposis: Analysis of three cases and a review of the literature. Oncol Lett 10: 2239-2243, 2015
APA
Kumamoto, K., Ishida, H., Ohsawa, T., Ishibashi, K., Ushiama, M., Yoshida, T., & Iwama, T. (2015). Germline and somatic mutations of the APC gene in papillary thyroid carcinoma associated with familial adenomatous polyposis: Analysis of three cases and a review of the literature. Oncology Letters, 10, 2239-2243. https://doi.org/10.3892/ol.2015.3578
MLA
Kumamoto, K., Ishida, H., Ohsawa, T., Ishibashi, K., Ushiama, M., Yoshida, T., Iwama, T."Germline and somatic mutations of the APC gene in papillary thyroid carcinoma associated with familial adenomatous polyposis: Analysis of three cases and a review of the literature". Oncology Letters 10.4 (2015): 2239-2243.
Chicago
Kumamoto, K., Ishida, H., Ohsawa, T., Ishibashi, K., Ushiama, M., Yoshida, T., Iwama, T."Germline and somatic mutations of the APC gene in papillary thyroid carcinoma associated with familial adenomatous polyposis: Analysis of three cases and a review of the literature". Oncology Letters 10, no. 4 (2015): 2239-2243. https://doi.org/10.3892/ol.2015.3578