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Compound Radix Sophorae Flavescentis exerts antitumor effects by inhibiting the proliferation and inducing the apoptosis of esophageal carcinoma ΤΕ-8 cells

  • Authors:
    • Xiaoyu Yang
    • Weimei Cai
    • Qinghui Yang
    • Zhihong Lu
    • Jinsong Li
    • Jian Yu
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Xinxiang Medical University, Xinxiang, Henan, P.R. China, The First Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2209-2213
    |
    Published online on: August 13, 2015
       https://doi.org/10.3892/ol.2015.3607
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Abstract

The aim of this study was to examine the effects of compound Radix Sophorae Flavescentis on the proliferation of esophageal carcinoma TE-8 cells and to elucidate the mechanisms involved. For this purpose, we incubated TE-8 cells in medium containing various concentrations (0, 0.0125, 0.025, 0.05, 0.1, 0.2, 0.4 and 0.8 mg/ml) of the compound Radix Sophorae Flavescentis injection and its effects on the proliferation of TE-8 cells were examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In addition, we observed the morphological changes and measured the expression levels of apoptosis-related genes (caspase-3, Bcl-2 and Bax) in the cells treated with different doses of the compound (low-dose group, 0.05 mg/ml; medium-dose group, 0.2 mg/ml; and high‑dose group, 0.8 ng/ml) by reverse transcription-quantitative PCR (RT-qPCR). The apoptotic index of the cancer cells treated with different doses of the compound was determined by TUNEL assay. Our results revealed that compared with the control group (untreated cells), the proliferation of the cancer cells treated with the compound was significantly inhibited (P≤0.05); the inhibition of the proliferation of the cancer cells occured in a dose-dependent manner. Compared with the control group, the apoptotic rate of the cells in the low-dose, medium-dose and high-dose groups increased significantly (P<0.05) in a dose-dependent manner. In addition, compared with the control group, the mRNA expression of caspase-3 and Bax increased significantly in the cells treated with the compound. However, the mRNA expression of Bcl-2 markedly decreased (P<0.05). With the gradual increase in the drug concentration, the mRNA expression levels of caspase-3, Bcl-2 and Bax in the cancer cells were altered in a dose‑dependent manner. In conclusion, our data demonstrate that compound Radix Sophorae Flavescentis injection significantly enhances the expression of pro-apoptotic genes in esophageal carcinoma TE-8 cells by increasing apoptosis and inhibiting cell proliferation. Thus, this study provides a theoretical basis for the clinical treatment of esophageal carcinoma.
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1 

Dongxin L: Esophageal cancer molecular epidemiology research in China. Zhonghua Liu Xing Bing Xue Za Zhi. 24:939–943. 2003.(In Chinese).

2 

Chen J, Zhu J, Pan J, Zhu K, Zheng X, Chen M, Wang J and Liao Z: Postoperative radiotherapy improved survival of poor prognostic squamous cell carcinoma esophagus. Ann Thorac Surg. 90:435–442. 2010. View Article : Google Scholar : PubMed/NCBI

3 

Mariette C, Piessen G and Triboulet JP: Therapeutic strategies in oesophageal carcinoma: Role of surgery and other modalities. Lancet Oncol. 8:545–553. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Ando N, Iizuka T, Ide H, Ishida K, Shinoda M, Nishimaki T, Takiyama W, Watanabe H, Isono K, Aoyama N, et al: Japan Clinical Oncology Group: Surgery plus chemotherapy compared with surgery alone for localized squamous cell carcinoma of the thoracic esophagus: A Japan Clinical Oncology Group Study - JCOG9204. J Clin Oncol. 21:4592–4596. 2003. View Article : Google Scholar : PubMed/NCBI

5 

Rice TW, Adelstein DJ, Chidel MA, Rybicki LA, DeCamp MM, Murthy SC and Blackstone EH: Benefit of postoperative adjuvant chemoradiotherapy in locoregionally advanced esophageal carcinoma. J Thorac Cardiovasc Surg. 126:1590–1596. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Qi L, Zhang J and Zhang Z: Determination of four alkaloids in compound Kushen Injection by high performance liquid chromatography with ionic liquid as mobile phase additive. Se Pu. 31:249–253. 2013.(In Chinese). PubMed/NCBI

7 

Dai ZJ, Gao J, Wang XJ, Ji ZZ, Wu WY, Liu XX, Kang HF, Guan HT and Ren HT: Apoptotic mechanism of gastric carcinoma cells induced by matrine injection. Zhonghua Wei Chang Wai Ke Za Zhi. 11:261–265. 2008.(In Chinese). PubMed/NCBI

8 

Dai ZJ, Gao J, Wu WY, Wang XJ, Li ZF, Kang HF, Liu XX and Ma XB: Effect of matrine injections on invasion and metastasis of gastric carcinoma SGC-7901 cells in vitro. Zhong Yao Cai. 30:815–819. 2007.(In Chinese). PubMed/NCBI

9 

Sun Q, Ma W, Gao Y, Zheng W, Zhang B and Peng Y: Meta-analysis: therapeutic effect of transcatheter arterial chemoembolization combined with compound kushen injection in hepatocellular carcinoma. Afr J Tradit Complement Altern Med. 9:178–188. 2012.PubMed/NCBI

10 

Zhu JL and Chen JZ: Short-term observation on the efficacy of matrine injection in treating 79 cases of late stage cancers. Shandong Med J. 43:article 40. 2003.

11 

Willert K, Brown JD, Danenberg E, Duncan AW, Weissman IL, Reya T, Yates JR III and Nusse R: Wnt proteins are lipid-modified and can act as stem cell growth factors. Nature. 423:448–452. 2003. View Article : Google Scholar : PubMed/NCBI

12 

Zhou H and Huang GH: Cognition and treatment research of traditional Chinese medicine for esophagus cancer. Liaoning Zhong Yi Yao Da Xue Xue Bao. 2:212–215. 2012.(In Chinese).

13 

Hai LN, Zhang ZW and Wang JH: Effects of compound Kushen injection on alalgesis, hemostasis and anti-stress in mice. Zhongguo Shi Yan Fang Ji Xue Za Zhi. 50:199–202. 2012.(In Chinese).

14 

Ma Y, Zhang QW, Wang ZM and Gao HM: Advance in study on compound Kushen injection. Zhongguo Shi Yan Fang Ji Xue Za Zhi. 18:23–24. 2012.

15 

Tuo XL and Bai M: Expression and significance of human mismatch repair gene (Smsh2) and proliferating cell nuclear antigen (PCNA) in lung cancer. China J Modem Med. 15:6912005.

16 

Simpson KL, Cawthorne C, Zhou C, Hodgkinson CL, Walker MJ, Trapani F, Kadirvel M, Brown G, Dawson MJ, MacFarlane M, et al: A caspase-3 ‘death-switch’ in colorectal cancer cells for induced and synchronous tumor apoptosis in vitro and in vivo facilitates the development of minimally invasive cell death biomarkers. Cell Death Dis. 4:e6132013. View Article : Google Scholar : PubMed/NCBI

17 

Raisova M, Hossini AM, Eberle J, Riebeling C, Wieder T, Sturm I, Daniel PT, Orfanos CE and Geilen CC: The Bax/Bcl-2 ratio determines the susceptibility of human melanoma cells to CD95/Fas-mediated apoptosis. J Invest Dermatol. 117:333–340. 2001. View Article : Google Scholar : PubMed/NCBI

18 

Salakou S, Kardamakis D, Tsamandas AC, Zolota V, Apostolakis E, Tzelepi V, Papathanasopoulos P, Bonikos DS, Papapetropoulos T, Petsas T, et al: Increased Bax/Bcl-2 ratio up-regulates caspase-3 and increases apoptosis in the thymus of patients with myasthenia gravis. In Vivo. 21:123–132. 2007.PubMed/NCBI

19 

Adams JM and Cory S: Bcl-2-regulated apoptosis: Mechanism and therapeutic potential. Curr Opin Immunol. 19:488–496. 2007. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yang X, Cai W, Yang Q, Lu Z, Li J and Yu J: Compound Radix Sophorae Flavescentis exerts antitumor effects by inhibiting the proliferation and inducing the apoptosis of esophageal carcinoma ΤΕ-8 cells. Oncol Lett 10: 2209-2213, 2015.
APA
Yang, X., Cai, W., Yang, Q., Lu, Z., Li, J., & Yu, J. (2015). Compound Radix Sophorae Flavescentis exerts antitumor effects by inhibiting the proliferation and inducing the apoptosis of esophageal carcinoma ΤΕ-8 cells. Oncology Letters, 10, 2209-2213. https://doi.org/10.3892/ol.2015.3607
MLA
Yang, X., Cai, W., Yang, Q., Lu, Z., Li, J., Yu, J."Compound Radix Sophorae Flavescentis exerts antitumor effects by inhibiting the proliferation and inducing the apoptosis of esophageal carcinoma ΤΕ-8 cells". Oncology Letters 10.4 (2015): 2209-2213.
Chicago
Yang, X., Cai, W., Yang, Q., Lu, Z., Li, J., Yu, J."Compound Radix Sophorae Flavescentis exerts antitumor effects by inhibiting the proliferation and inducing the apoptosis of esophageal carcinoma ΤΕ-8 cells". Oncology Letters 10, no. 4 (2015): 2209-2213. https://doi.org/10.3892/ol.2015.3607
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Cai W, Yang Q, Lu Z, Li J and Yu J: Compound Radix Sophorae Flavescentis exerts antitumor effects by inhibiting the proliferation and inducing the apoptosis of esophageal carcinoma ΤΕ-8 cells. Oncol Lett 10: 2209-2213, 2015.
APA
Yang, X., Cai, W., Yang, Q., Lu, Z., Li, J., & Yu, J. (2015). Compound Radix Sophorae Flavescentis exerts antitumor effects by inhibiting the proliferation and inducing the apoptosis of esophageal carcinoma ΤΕ-8 cells. Oncology Letters, 10, 2209-2213. https://doi.org/10.3892/ol.2015.3607
MLA
Yang, X., Cai, W., Yang, Q., Lu, Z., Li, J., Yu, J."Compound Radix Sophorae Flavescentis exerts antitumor effects by inhibiting the proliferation and inducing the apoptosis of esophageal carcinoma ΤΕ-8 cells". Oncology Letters 10.4 (2015): 2209-2213.
Chicago
Yang, X., Cai, W., Yang, Q., Lu, Z., Li, J., Yu, J."Compound Radix Sophorae Flavescentis exerts antitumor effects by inhibiting the proliferation and inducing the apoptosis of esophageal carcinoma ΤΕ-8 cells". Oncology Letters 10, no. 4 (2015): 2209-2213. https://doi.org/10.3892/ol.2015.3607
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