Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
November-2015 Volume 10 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
November-2015 Volume 10 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Serum miRNA expression in patients with esophageal squamous cell carcinoma

  • Authors:
    • Beina Hui
    • Xin Chen
    • Lingyun Hui
    • Ruxing Xi
    • Xiaozhi Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, First Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, P.R. China, Department of Clinical Laboratory, First Affiliated Hospital of Xi'an Jiaotong University School of Medicine, Xi'an, Shaanxi 710061, P.R. China
  • Pages: 3008-3012
    |
    Published online on: August 25, 2015
       https://doi.org/10.3892/ol.2015.3642
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to investigate the feasibility and value of serum microRNAs (miRNAs/miRs) as biological markers for the prediction of the behavior and prognosis of esophageal squamous cell cancer (ESCC). The differential expression of serum miRNA was detected by an miRNA microarray of 9 patients with ESCC and 9 healthy volunteers. The result of the miRNA microarray was validated in serum samples of 69 patients with ESCC and 14 healthy volunteers by reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). The association between serum miRNA expression and tumor‑node‑metastasis (TNM) stage was analyzed. A total of 10 serum‑specific miRNAs were identified from the patients with ESCC. Through PCR verification, the expression levels of miR‑129, miR‑451 and miR‑365 were consistent with the microarray results validated by RT‑qPCR, and the difference was statistically significant compared with the healthy volunteers (P=0.007, P=0.007 and P<0.001, respectively). Multivariate logistic regression analysis showed that miR‑365 could serve as potential diagnostic marker for ESCC; the area under the receiver operating characteristic curve was 0.831, with a sensitivity of 80.56% and a specificity of 86.7%, but its expression did not differ significantly among the different TNM stages (stage I‑II vs. III, P=0.052; stage III vs. IV, P=0.069). The expression level of miRNA‑129 differed significantly among the different stages (stage I‑II vs. III, , P=0.002; stage III vs. IV, P=0.042), while the expression level of miR‑451 did not differ significantly between stage III and IV (P=0.308). In conclusion, serum microRNAs are novel biomarkers for ESCC, and miRNA‑365 and miRNA‑129 can be used for the early prediction of cancer and the prediction of clinical stage, respectively.
View Figures

Figure 1

Figure 2

Figure 3

View References

1 

Chen W, Zheng R, Zhang S, Zhao P, Zeng H, Zou X and He J: Annual report on status of cancer in China, 2010. Chin J Cancer Res. 26:48–58. 2014.PubMed/NCBI

2 

Wang GQ, Jiao GG, Chang FB, et al: Long-term results of operation for 420 patients with early squamous cell esophageal carcinoma discovered by screening. Ann Throac surg. 77:1740–1744. 2004. View Article : Google Scholar

3 

Campbell F, Lauwers GY and Williams GT: Tumors of the esophagus and stomach. Diagnostic Histopathology of Tumors. Fletcher CDM: (3rd). Churchill Livingstone Elsevier. (London). 328–329. 2007.

4 

Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Resinick KE, Alder H, Hagan JP, et al: The detection of differentially expressed microRNA from the serum of ovarian cancer patients using a novel real-time PCR platform. Gynecol Oncol. 112:55–59. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Taylor DD and Gercel-Taylor C: MicroRNA signatures of tumor-derived exosomes as diagnostic biomarkers of ovarian cancer. Gynecol Oncol. 110:13–21. 2008. View Article : Google Scholar : PubMed/NCBI

7 

Lawrie CH, Gal S, Dunlop HM, et al: Detection of elevated levels of tumor-associated microRNAs in serum of patients with diffuse large B-cell lymphoma. Br J Haematol. 141:672–675. 2008. View Article : Google Scholar : PubMed/NCBI

8 

Chin LJ and Slack FJ: A truth serum for cancer-microRNAs have major potential as cancer biomarkers. Cell Res. 18:983–984. 2008. View Article : Google Scholar : PubMed/NCBI

9 

Zhou J, Yu L, Gao X, et al: A Plasma MicroRNA panel to diagnose hepatitis B virus related hepatocellular carcinoma. J Clin Oncol. 29:4781–4788. 2011. View Article : Google Scholar : PubMed/NCBI

10 

Hu J, Wang Z, Liao BY, et al: Human miR-1228 as a stable endogenous control for the quantification of circulating microRNAs in cancer patients. Int J Cancer. 135:1187–1194. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Pigati L, Yaddanapudi SC, Iyengar R, Kim DJ, Hearn SA, Danforth D, Hastings ML and Duelli DM: Selective release of microRNA species from normal and malignant mammary epithelial cells. PLoS One. 5:e135152010. View Article : Google Scholar : PubMed/NCBI

12 

Iguchi H, Kosaka N and Ochiya T: Secretory microRNAs as a versatile communication tool. Commun Integr Biol. 3:478–481. 2010. View Article : Google Scholar : PubMed/NCBI

13 

Zhou M, Liu W, Ma S, et al: A novel onco-miR-365 induces cutaneous squamous cell carcinoma. Carcinogenesis. 34:1653–1659. 2013. View Article : Google Scholar : PubMed/NCBI

14 

Ogawa R, Ishiguro H, Kuwabara Y, et al: Expression profilinf of micro-RNAs in human esophageal squamous cell carcinoma using RT-PCR. Med Mol Morphol. 42:102–109. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Pan X, Wang R and Wang ZX: The potential role of miR-451 in cancer diagnosis, prognosis, and therapy. Mol Cancer Ther. 12:1153–1162. 2013. View Article : Google Scholar : PubMed/NCBI

16 

Kovalchuk O, Filkowski J, Meservy J, et al: Involvement of microRNA-451 in resistance of the MCF-7 breast cancer cells to chemotherapeutic drug doxorubicin. Mol Cancer Ther. 7:2152–2159. 2008. View Article : Google Scholar : PubMed/NCBI

17 

Zhu H, Wu H, Liu X, et al: Role of MicroRNA miR-27a and miR-451 in the regulation of MDR1/P-glycoprotein expression in human cancer cells. Biochem Pharmacol. 76:582–588. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Wang XC, Tian LL, Jiang XY, et al: The expression and function of miRNA-451 in non-small cell lung cancer. Cancer Lett. 311:203–209. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Banders E, Bitarte N, Arias F, et al: MicroRNA-451 regulates macrophage migration inhibitory factor production and proliferation of gastrointestinal cancer cells. Clin Cancer Res. 15:2281–2290. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Wang R, Wang ZX, Yang JS, Pan X, De W and Chen LB: MicroRNA-451 functions as a tumor suppressor in human non-small cell lung cancer by targeting ras-related protein 14 (RAB14). Oncogene. 30:2644–2658. 2011. View Article : Google Scholar : PubMed/NCBI

21 

Hui AB, Lenarduzzi M, Krushel T, et al: Comprehensive MicroRNA profiling for head and neck squamous cell carcinomas. Clin Cancer Res. 16:1129–1139. 2010. View Article : Google Scholar : PubMed/NCBI

22 

Wang T, Zang WQ, Li M, Wang N, Zheng YL and Zhao GQ: Effect of miR-451 on the biological behavior of esophageal carcinoma cell line EC9706. Dig Dis Sci. 58:706–714. 2013. View Article : Google Scholar : PubMed/NCBI

23 

Tanaka Y, Kamohara H, Kinoshita K, et al: Clinical impact of serum exosomal microRNA-21 as a clinical biomarker in human esophageal squamous cell carcinoma. Cancer. 119:1159–1167. 2013. View Article : Google Scholar : PubMed/NCBI

24 

Cai EH, Gao YX, Wei ZZ, Chen WY, Yu P and Li K: Serum miR-21 expression in human esophageal squamous cell carcinomas. Asian Pac J Cancer Prev. 13:1563–1567. 2012. View Article : Google Scholar : PubMed/NCBI

25 

Kurashige J, Kamohara H, Watanabe M, et al: Serum microRNA-21 is a novel biomarker in patients with esophageal squamous cell carcinoma. J Surg Oncol. 106:188–192. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Basati G, Razavi A Emami, Abdi S and Mirzaei A: Elevated level of microRNA-21 in the serum of patients with colorectal cancer. Med Oncol. 31:2052014. View Article : Google Scholar : PubMed/NCBI

27 

Yang X, Guo Y, Du Y, et al: Serum microRNA-21 as a diagnostic marker for lung carcinoma: A systematic review and meta-analysis. PLoS One. 9:e974602014. View Article : Google Scholar : PubMed/NCBI

28 

Song J, Bai Z, Zhang J, et al: Serum microRNA-21 levels are related to tumor size in gastric cancer patients but cannot predict prognosis. Oncol Lett. 6:1733–1737. 2013.PubMed/NCBI

29 

Zhang C, Wang C, Chen X, et al: Expression profile of microRNAs in serum: A fingerprint for esophageal squamous cell carcinoma. Clin Chem. 56:1871–1879. 2010. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Hui B, Chen X, Hui L, Xi R and Zhang X: Serum miRNA expression in patients with esophageal squamous cell carcinoma. Oncol Lett 10: 3008-3012, 2015.
APA
Hui, B., Chen, X., Hui, L., Xi, R., & Zhang, X. (2015). Serum miRNA expression in patients with esophageal squamous cell carcinoma. Oncology Letters, 10, 3008-3012. https://doi.org/10.3892/ol.2015.3642
MLA
Hui, B., Chen, X., Hui, L., Xi, R., Zhang, X."Serum miRNA expression in patients with esophageal squamous cell carcinoma". Oncology Letters 10.5 (2015): 3008-3012.
Chicago
Hui, B., Chen, X., Hui, L., Xi, R., Zhang, X."Serum miRNA expression in patients with esophageal squamous cell carcinoma". Oncology Letters 10, no. 5 (2015): 3008-3012. https://doi.org/10.3892/ol.2015.3642
Copy and paste a formatted citation
x
Spandidos Publications style
Hui B, Chen X, Hui L, Xi R and Zhang X: Serum miRNA expression in patients with esophageal squamous cell carcinoma. Oncol Lett 10: 3008-3012, 2015.
APA
Hui, B., Chen, X., Hui, L., Xi, R., & Zhang, X. (2015). Serum miRNA expression in patients with esophageal squamous cell carcinoma. Oncology Letters, 10, 3008-3012. https://doi.org/10.3892/ol.2015.3642
MLA
Hui, B., Chen, X., Hui, L., Xi, R., Zhang, X."Serum miRNA expression in patients with esophageal squamous cell carcinoma". Oncology Letters 10.5 (2015): 3008-3012.
Chicago
Hui, B., Chen, X., Hui, L., Xi, R., Zhang, X."Serum miRNA expression in patients with esophageal squamous cell carcinoma". Oncology Letters 10, no. 5 (2015): 3008-3012. https://doi.org/10.3892/ol.2015.3642
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team