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Article

miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression

  • Authors:
    • Kun Duan
    • Yong‑Chao Ge
    • Xue‑Pei Zhang
    • Shu‑Yi Wu
    • Jin‑Shun Feng
    • Shi‑Lin Chen
    • Li Zhang
    • Zhi‑Hao Yuan
    • Chao‑Hong Fu
  • View Affiliations / Copyright

    Affiliations: Department of Urology Surgery, The Third People's Hospital of Zhengzhou, Zhengzhou, Henan, P.R. China, Department of Urology Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, P.R. China, Department of Stem Cell and Organ Transplantation Laboratory, The Third People's Hospital of Zhengzhou, Zhengzhou, Henan, P.R. China
  • Pages: 3223-3227
    |
    Published online on: August 26, 2015
       https://doi.org/10.3892/ol.2015.3645
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Abstract

MicroRNA-34a (miR-34a) functions as a tumor suppressor gene and inhibits abnormal cell growth by regulating the expression of other genes. The role of miR‑34a in regulating sirtuin 1 (SIRT1) in prostate cancer remains unclear. The objective of the present study was to investigate the biological function and molecular mechanisms of miR‑34a regulation of SIRT1 in human prostate cancer samples and the human prostate cancer cell line, PC‑3. Fresh prostate tissues were obtained from patients, and the miR‑34a expression in prostate cancer tissues was measured using reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR). qPCR and western blotting were performed to assess the effects of miR‑34a overexpression on SIRT1 regulation in PC‑3 cells, and the cell growth was assessed by Cell Counting Kit‑8 (CCK‑8). Flow cytometry was used to assess the cell cycle status of the cells. The miR‑34a expression levels in prostate cancer tissues were significantly reduced compared with adjacent normal prostate tissues (P<0.05). SIRT1 expression levels in PC‑3 cells with over‑expression of miR‑34a were significantly reduced compared with those in the negative control (P<0.05). The over‑expression of miR‑34a inhibited PC‑3 cells growth and resulted in increased cell cycle arrest compared with the negative control (P<0.05). In conclusion, miR‑34a inhibits the human prostate cancer cell proliferation, in part, through the downregulation of SIRT1 expression.
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Copy and paste a formatted citation
Spandidos Publications style
Duan K, Ge YC, Zhang XP, Wu SY, Feng JS, Chen SL, Zhang L, Yuan ZH and Fu CH: miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression. Oncol Lett 10: 3223-3227, 2015.
APA
Duan, K., Ge, Y., Zhang, X., Wu, S., Feng, J., Chen, S. ... Fu, C. (2015). miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression. Oncology Letters, 10, 3223-3227. https://doi.org/10.3892/ol.2015.3645
MLA
Duan, K., Ge, Y., Zhang, X., Wu, S., Feng, J., Chen, S., Zhang, L., Yuan, Z., Fu, C."miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression". Oncology Letters 10.5 (2015): 3223-3227.
Chicago
Duan, K., Ge, Y., Zhang, X., Wu, S., Feng, J., Chen, S., Zhang, L., Yuan, Z., Fu, C."miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression". Oncology Letters 10, no. 5 (2015): 3223-3227. https://doi.org/10.3892/ol.2015.3645
Copy and paste a formatted citation
x
Spandidos Publications style
Duan K, Ge YC, Zhang XP, Wu SY, Feng JS, Chen SL, Zhang L, Yuan ZH and Fu CH: miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression. Oncol Lett 10: 3223-3227, 2015.
APA
Duan, K., Ge, Y., Zhang, X., Wu, S., Feng, J., Chen, S. ... Fu, C. (2015). miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression. Oncology Letters, 10, 3223-3227. https://doi.org/10.3892/ol.2015.3645
MLA
Duan, K., Ge, Y., Zhang, X., Wu, S., Feng, J., Chen, S., Zhang, L., Yuan, Z., Fu, C."miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression". Oncology Letters 10.5 (2015): 3223-3227.
Chicago
Duan, K., Ge, Y., Zhang, X., Wu, S., Feng, J., Chen, S., Zhang, L., Yuan, Z., Fu, C."miR-34a inhibits cell proliferation in prostate cancer by downregulation of SIRT1 expression". Oncology Letters 10, no. 5 (2015): 3223-3227. https://doi.org/10.3892/ol.2015.3645
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