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Article

Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes

  • Authors:
    • Cong Zu
    • Mingdi Zhang
    • Hui Xue
    • Xiaopeng Cai
    • Lei Zhao
    • Anning He
    • Guangyuan Qin
    • Chunshu Yang
    • Xinyu Zheng
  • View Affiliations / Copyright

    Affiliations: Lab 1, Cancer Institute, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of General Surgery, Xinhua Hospital, Shanghai Jiaotong University, School of Medicine, Shanghai 200092, P.R. China, Department of Gynecology, First Affiliated Hospital of China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Surgical Oncology, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430072, P.R. China, Center of Experiment Technology and Medical Research, China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 2919-2924
    |
    Published online on: August 26, 2015
       https://doi.org/10.3892/ol.2015.3646
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Abstract

The aim of this study was to investigate the effects of emodin on the proliferation of human breast cancer cells Bcap-37 and ZR-75-30. Cell viability following emodin treatment was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of emodin on apoptosis were determined by flow cytometry using Annexin V‑fluorescein isothiocyanate and propidium iodide staining. Quantitative polymerase chain reaction and western blot analysis were used to determine changes in the expression of apoptotic genes and protein, respectively. The effect of emodin on the invasiveness of breast cancer cells was evaluated by Matrigel invasion assay. Treatment of breast cancer cells Bcap‑37 and ZR‑75‑30 with emodin was observed to inhibit the growth and induced apoptosis in a time‑ and dose‑dependent manner. Emodin reduced the level of Bcl‑2 and increased levels of cleaved caspase‑3, PARP, p53 and Bax. These findings indicate that emodin induces growth inhibition and apoptosis in human breast cancer cells. Emodin may be a potential therapeutic agent for the treatment of breast cancer.
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1 

Sanguinetti A, Polistena A, Lucchini R, Monacelli M, Avenia S, Conti C, Barillaro I, Rondelli F, Bugiantella W and Avenia N: Breast cancer in older women: What factors affect the treatment? Int J Surg. 12(Suppl 2): S177–S180. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Ferlay JSI, Ervik M, Dikshit R, Eser S, Mathers C, Rebelo M, Parkin DM, Forman D and Bray F: GLOBOCAN 2012 v1.0, Cancer Incidence and Mortality Worldwide: IARC CancerBase No. 11. (Lyon, France). International Agency for Research on Cancer. 2013.http://globocan.iarc.frAccessed. December 12–2013

3 

Zeng H, Zheng R, Zhang S, Zou X and Chen W: Female breast cancer statistics of 2010 in China: Estimates based on data from 145 population-based cancer registries. J Thorac Dis. 6:466–470. 2014.PubMed/NCBI

4 

Lin G, Zhu W, Yang L, Wu J, Lin B, Xu Y, Cheng Z, Xia C, Gong Q, Song B and Ai H: Delivery of siRNA by MRI-visible nanovehicles to overcome drug resistance in MCF-7/ADR human breast cancer cells. Biomaterials. 35:9495–9507. 2014. View Article : Google Scholar : PubMed/NCBI

5 

Amirzada MI, Ma X, Gong X, Chen Y, Bashir S and Jin J: Recombinant human interleukin 24 reverses Adriamycin resistance in a human breast cancer cell line. Pharmacol Rep. 66:915–919. 2014. View Article : Google Scholar : PubMed/NCBI

6 

Bright-Gbebry M, Makambi KH, Rohan JP, Llanos AA, Rosenberg L, Palmer JR and Adams-Campbell LL: Use of multivitamins, folic acid and herbal supplements among breast cancer survivors: the black women's health study. BMC Complement Altern Med. 11:302011. View Article : Google Scholar : PubMed/NCBI

7 

Spazzapan S, Crivellari D, Bedard P, Lombardi D, Miolo G, Scalone S and Veronesi A: Therapeutic management of breast cancer in the elderly. Expert Opin Pharmacother. 12:945–960. 2011. View Article : Google Scholar : PubMed/NCBI

8 

Li WY, Chan SW, Guo DJ, Chung MK, Leung TY and Yu PH: Water extract of Rheum officinale Baill. induces apoptosis in human lung adenocarcinoma A549 and human breast cancer MCF-7 cell lines. J Ethnopharmacol. 124:251–256. 2009. View Article : Google Scholar : PubMed/NCBI

9 

Wang C, Wu X, Chen M, Duan W, Sun L, Yan M and Zhang L: Emodin induces apoptosis through caspase 3-dependent pathway in HK-2 cells. Toxicology. 231:120–128. 2007. View Article : Google Scholar : PubMed/NCBI

10 

Li WY, Ng YF, Zhang H, Guo ZD, Guo DJ, Kwan YW, Leung GP, Lee SM, Yu PH and Chan SW: Emodin elicits cytotoxicity in human lung adenocarcinoma A549 cells through inducing apoptosis. Inflammopharmacology. 22:127–134. 2014. View Article : Google Scholar : PubMed/NCBI

11 

Li WY, Chan RY, Yu PH and Chan SW: Emodin induces cytotoxic effect in human breast carcinoma MCF-7 cell through modulating the expression of apoptosis-related genes. Pharm Biol. 51:1175–1181. 2013. View Article : Google Scholar : PubMed/NCBI

12 

Sui JQ, Xie KP, Zou W and Xie MJ: Emodin inhibits breast cancer cell proliferation through the ERα-MAPK/Akt-cyclin D1/Bcl-2 signaling pathway. Asian Pac J Cancer Prev. 15:6247–6251. 2014. View Article : Google Scholar : PubMed/NCBI

13 

Pooja T and Karunagaran D: Emodin suppresses Wnt signaling in human colorectal cancer cells SW480 and SW620. Eur J Pharmacol. 742:55–64. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Wei WT, Lin SZ, Liu DL and Wang ZH: The distinct mechanisms of the antitumor activity of emodin in different types of cancer (Review). Oncol Rep. 30:2555–2562. 2013.PubMed/NCBI

15 

Xu JD, Liu S, Wang W, Li LS, Li XF, Li Y, Guo H, Ji T, Feng XY, Hou XL, et al: Emodin induces chloride secretion in rat distal colon through activation of mast cells and enteric neurons. Br J Pharmacol. 165:197–207. 2012. View Article : Google Scholar : PubMed/NCBI

16 

Xue H, Chen Y, Cai X, Zhao L, He A, Guo K and Zheng X: The combined effect of survivin-targeted shRNA and emodin on the proliferation and invasion of ovarian cancer cells. Anticancer Drugs. 24:937–944. 2013. View Article : Google Scholar : PubMed/NCBI

17 

Yaoxian W, Hui Y, Yunyan Z, Yanqin L, Xin G and Xiaoke W: Emodin induces apoptosis of human cervical cancer hela cells via intrinsic mitochondrial and extrinsic death receptor pathway. Cancer Cell Int. 13:712013. View Article : Google Scholar : PubMed/NCBI

18 

Way TD, Huang JT, Chou CH, Huang CH, Yang MH and Ho CT: Emodin represses TWIST1-induced epithelial-mesenchymal transitions in head and neck squamous cell carcinoma cells by inhibiting the β-catenin and Akt pathways. Eur J Cancer. 50:366–378. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Lin SZ, Wei WT, Chen H, Chen KJ, Tong HF, Wang ZH, Ni ZL, Liu HB, Guo HC and Liu DL: Antitumor activity of emodin against pancreatic cancer depends on its dual role: Promotion of apoptosis and suppression of angiogenesis. PLoS One. 7:e421462012. View Article : Google Scholar : PubMed/NCBI

20 

Muppidi J, Porter M and Siegel RM: Measurement of apoptosis and other forms of cell death. Curr Protoc Immunol. View Article : Google Scholar : PubMed/NCBI

21 

Evan GI and Vousden KH: Proliferation, cell cycle and apoptosis in cancer. Nature. 411:342–348. 2001. View Article : Google Scholar : PubMed/NCBI

22 

Kelly PN and Strasser A: The role of Bcl-2 and its pro-survival relatives in tumourigenesis and cancer therapy. Cell Death Differ. 18:1414–1424. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Strasser A, Cory S and Adams JM: Deciphering the rules of programmed cell death to improve therapy of cancer and other diseases. EMBO J. 30:3667–3683. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Heath RM, Jayne DG, O'Leary R, Morrison EE and Guillou PJ: Tumour-induced apoptosis in human mesothelial cells: A mechanism of peritoneal invasion by Fas Ligand/Fas interaction. Br J Cancer. 90:1437–1442. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Geng QQ, Dong DF, Chen NZ, Wu YY, Li EX, Wang J and Wang SM: Induction of p53 expression and apoptosis by a recombinant dual-target MDM2/MDMX inhibitory protein in wild-type p53 breast cancer cells. Int J Oncol. 43:1935–1942. 2013.PubMed/NCBI

26 

Harris SL and Levine AJ: The p53 pathway: positive and negative feedback loops. Oncogene. 24:2899–2908. 2005. View Article : Google Scholar : PubMed/NCBI

27 

Leard LE and Broaddus VC: Mesothelial cell proliferation and apoptosis. Respirology. 9:292–299. 2004. View Article : Google Scholar : PubMed/NCBI

28 

Fang CC, Yen CJ, Tsai TJ, Chen RH, Lee PH and Tomino Y: Antibiotics induce apoptosis of human peritoneal mesothelial cells. Nephrology (Carlton). 8:142–149. 2003. View Article : Google Scholar : PubMed/NCBI

29 

Korsmeyer SJ, Shutter JR, Veis DJ, Merry DE and Oltvai ZN: Bcl-2/Bax: a rheostat that regulates an anti-oxidant pathway and cell death. Semin Cancer Biol. 4:327–332. 1993.PubMed/NCBI

30 

Lindsay J, Esposti MD and Gilmore AP: Bcl-2 proteins and mitochondria-specificity in membrane targeting for death. Biochim Biophys Acta. 1813:532–539. 2011. View Article : Google Scholar : PubMed/NCBI

31 

Ma L and Li W: Emodin inhibits LOVO colorectal cancer cell proliferation via the regulation of the Bcl-2/Bax ratio and cytochrome c. Exp Ther Med. 8:1225–1228. 2014.PubMed/NCBI

32 

Wang X: The expanding role of mitochondria in apoptosis. Genes Dev. 15:2922–2933. 2001.PubMed/NCBI

33 

Liu TY, Tan ZJ, Jiang L, Gu JF, Wu XS, Cao Y, Li ML, Wu KJ and Liu YB: Curcumin induces apoptosis in gallbladder carcinoma cell line GBC-SD cells. Cancer Cell Int. 13:642013. View Article : Google Scholar : PubMed/NCBI

34 

Liu J, Wu N, Ma LN, Zhong JT, Liu G, Zheng LH and Lin XK: p38 MAPK signaling mediates mitochondrial apoptosis in cancer cells induced by oleanolic acid. Asian Pac J Cancer Prev. 15:4519–4525. 2014. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zu C, Zhang M, Xue H, Cai X, Zhao L, He A, Qin G, Yang C and Zheng X: Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes. Oncol Lett 10: 2919-2924, 2015.
APA
Zu, C., Zhang, M., Xue, H., Cai, X., Zhao, L., He, A. ... Zheng, X. (2015). Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes. Oncology Letters, 10, 2919-2924. https://doi.org/10.3892/ol.2015.3646
MLA
Zu, C., Zhang, M., Xue, H., Cai, X., Zhao, L., He, A., Qin, G., Yang, C., Zheng, X."Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes". Oncology Letters 10.5 (2015): 2919-2924.
Chicago
Zu, C., Zhang, M., Xue, H., Cai, X., Zhao, L., He, A., Qin, G., Yang, C., Zheng, X."Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes". Oncology Letters 10, no. 5 (2015): 2919-2924. https://doi.org/10.3892/ol.2015.3646
Copy and paste a formatted citation
x
Spandidos Publications style
Zu C, Zhang M, Xue H, Cai X, Zhao L, He A, Qin G, Yang C and Zheng X: Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes. Oncol Lett 10: 2919-2924, 2015.
APA
Zu, C., Zhang, M., Xue, H., Cai, X., Zhao, L., He, A. ... Zheng, X. (2015). Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes. Oncology Letters, 10, 2919-2924. https://doi.org/10.3892/ol.2015.3646
MLA
Zu, C., Zhang, M., Xue, H., Cai, X., Zhao, L., He, A., Qin, G., Yang, C., Zheng, X."Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes". Oncology Letters 10.5 (2015): 2919-2924.
Chicago
Zu, C., Zhang, M., Xue, H., Cai, X., Zhao, L., He, A., Qin, G., Yang, C., Zheng, X."Emodin induces apoptosis of human breast cancer cells by modulating the expression of apoptosis-related genes". Oncology Letters 10, no. 5 (2015): 2919-2924. https://doi.org/10.3892/ol.2015.3646
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