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MicroRNA-34a-5p enhances sensitivity to chemotherapy by targeting AXL in hepatocellular carcinoma MHCC-97L cells

  • Authors:
    • Xiao‑Yun Li
    • Jing‑Yun Wen
    • Chang‑Chang Jia
    • Tian‑Tian Wang
    • Xing Li
    • Min Dong
    • Qu Lin
    • Zhan‑Hong Chen
    • Xiao‑Kun Ma
    • Li Wei
    • Ze‑Xiao Lin
    • Dan‑Yun Ruan
    • Jie Chen
    • Dong‑Hao Wu
    • Wei Liu
    • Yan Tai
    • Zhi‑Yong Xiong
    • Xiang‑Yuan Wu
    • Qi Zhang

  • View Affiliations / Copyright

    Affiliations: Department of Medical Oncology, The Third Affiliated Hospital of Sun Yat‑sen University, Guangzhou, Gunagdong 510630, P.R. China, Guangdong Provincial Key Laboratory of Liver Disease Research, Sun Yat‑sen University, Guangzhou, Gunagdong 510630, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 2691-2698
    |
    Published online on: August 28, 2015
       https://doi.org/10.3892/ol.2015.3654
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Abstract

Mature microRNA (miRNA) 34a-5p, which is a well-known tumor suppressor in hepatitis virus‑associated hepatocellular carcinoma (HCC), plays an important role in cell processes, such as cell proliferation and apoptosis, and is therefore an optimal biomarker for future clinical use. However, the role of miRNA‑34a‑5p in chemoresistance has yet to be identified. In the present study, the expression of miRNA‑34a‑5p was assessed by an in situ hybridization assay in HCC tissues and was found to be significantly decreased compared with the pericarcinomatous areas of the tissue specimens, which consisted of samples obtained from 114 patients with HCC. High expression of miRNA‑34a‑5p was found to be associated with a favorable overall survival time in HCC patients. Functional tests performed by transfecting miRNA‑34a‑5p mimics or inhibitors into MHCC‑97L cells illustrated that miRNA‑34a‑5p inhibited proliferation, elevated apoptosis and decreased chemoresistance to cisplatin in HCC cells. AXL is the direct target of miRNA‑34a‑5p, as confirmed by sequence analysis and luciferase assay. Transfection of the cells with small interfering RNA for AXL (siAXL) increased the apoptosis ratio of the MHCC‑97L cell line. Transfection with siAXL led to similar biological behaviors in the MHCC‑97L cells to those induced by ectopic expression of miRNA‑34a‑5p. Thus, it was concluded that miRNA‑34a‑5p enhanced the sensitivity of the cells to chemotherapy by targeting AXL in hepatocellular carcinoma. In addition, low expression of miRNA‑34a‑5p in HCC tissues yielded an unfavorable prognosis for patients with HCC that received radical surgery, due to the promotion of proliferation and an increase in chemoresistance in HCC cells.
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Copy and paste a formatted citation
Spandidos Publications style
Li XY, Wen JY, Jia CC, Wang TT, Li X, Dong M, Lin Q, Chen ZH, Ma XK, Wei L, Wei L, et al: MicroRNA-34a-5p enhances sensitivity to chemotherapy by targeting AXL in hepatocellular carcinoma MHCC-97L cells. Oncol Lett 10: 2691-2698, 2015.
APA
Li, X., Wen, J., Jia, C., Wang, T., Li, X., Dong, M. ... Zhang, Q. (2015). MicroRNA-34a-5p enhances sensitivity to chemotherapy by targeting AXL in hepatocellular carcinoma MHCC-97L cells. Oncology Letters, 10, 2691-2698. https://doi.org/10.3892/ol.2015.3654
MLA
Li, X., Wen, J., Jia, C., Wang, T., Li, X., Dong, M., Lin, Q., Chen, Z., Ma, X., Wei, L., Lin, Z., Ruan, D., Chen, J., Wu, D., Liu, W., Tai, Y., Xiong, Z., Wu, X., Zhang, Q."MicroRNA-34a-5p enhances sensitivity to chemotherapy by targeting AXL in hepatocellular carcinoma MHCC-97L cells". Oncology Letters 10.5 (2015): 2691-2698.
Chicago
Li, X., Wen, J., Jia, C., Wang, T., Li, X., Dong, M., Lin, Q., Chen, Z., Ma, X., Wei, L., Lin, Z., Ruan, D., Chen, J., Wu, D., Liu, W., Tai, Y., Xiong, Z., Wu, X., Zhang, Q."MicroRNA-34a-5p enhances sensitivity to chemotherapy by targeting AXL in hepatocellular carcinoma MHCC-97L cells". Oncology Letters 10, no. 5 (2015): 2691-2698. https://doi.org/10.3892/ol.2015.3654
Copy and paste a formatted citation
x
Spandidos Publications style
Li XY, Wen JY, Jia CC, Wang TT, Li X, Dong M, Lin Q, Chen ZH, Ma XK, Wei L, Wei L, et al: MicroRNA-34a-5p enhances sensitivity to chemotherapy by targeting AXL in hepatocellular carcinoma MHCC-97L cells. Oncol Lett 10: 2691-2698, 2015.
APA
Li, X., Wen, J., Jia, C., Wang, T., Li, X., Dong, M. ... Zhang, Q. (2015). MicroRNA-34a-5p enhances sensitivity to chemotherapy by targeting AXL in hepatocellular carcinoma MHCC-97L cells. Oncology Letters, 10, 2691-2698. https://doi.org/10.3892/ol.2015.3654
MLA
Li, X., Wen, J., Jia, C., Wang, T., Li, X., Dong, M., Lin, Q., Chen, Z., Ma, X., Wei, L., Lin, Z., Ruan, D., Chen, J., Wu, D., Liu, W., Tai, Y., Xiong, Z., Wu, X., Zhang, Q."MicroRNA-34a-5p enhances sensitivity to chemotherapy by targeting AXL in hepatocellular carcinoma MHCC-97L cells". Oncology Letters 10.5 (2015): 2691-2698.
Chicago
Li, X., Wen, J., Jia, C., Wang, T., Li, X., Dong, M., Lin, Q., Chen, Z., Ma, X., Wei, L., Lin, Z., Ruan, D., Chen, J., Wu, D., Liu, W., Tai, Y., Xiong, Z., Wu, X., Zhang, Q."MicroRNA-34a-5p enhances sensitivity to chemotherapy by targeting AXL in hepatocellular carcinoma MHCC-97L cells". Oncology Letters 10, no. 5 (2015): 2691-2698. https://doi.org/10.3892/ol.2015.3654
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