Carboxy‑terminal domain phosphatase 1 silencing results in the inhibition of tumor formation ability in gastric cancer cells

Fu,Hongbing; Yang,Dejun; Wang,Changming; Xu,Jiapeng; Wang,Weimin; Yan,Ronglin; Cai,Qingping

doi:10.3892/ol.2015.3693

November-2015 Volume 10 Issue 5

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

November-2015 Volume 10 Issue 5

Full Size Image

Article

Carboxy‑terminal domain phosphatase 1 silencing results in the inhibition of tumor formation ability in gastric cancer cells

Authors:
- Hongbing Fu
- Dejun Yang
- Changming Wang
- Jiapeng Xu
- Weimin Wang
- Ronglin Yan
- Qingping Cai
View Affiliations / Copyright

Affiliations: Department of Gastrointestinal Surgery, Shanghai Changzheng Hospital, Second Military Medical University, Shanghai 200003, P.R. China
Pages: 2947-2952
|
Published online on: September 10, 2015

https://doi.org/10.3892/ol.2015.3693
Expand metrics +

Abstract

Gastric cancer (GC), one of the most malignant types of cancer, is the second greatest cause of cancer‑associated mortality worldwide. Novel therapeutic targets for GC treatment are therefore urgently required. Carboxy‑terminal domain phosphatase 1 (CTDP1) has a crucial role in the regulation of gene expression. However, to the best of our knowledge, the role of CTDP1 in GC has not previously been explored. In the present study, reverse transcription‑quantitative polymerase chain reaction analysis was used to detect CTDP1 messenger RNA expression in various GC cell lines. CTDP1 was subsequently silenced in GC cells by lentivirus‑mediated small interfering RNA (siRNA) infection, and the effects of CTDP1 inhibition on cell proliferation were evaluated by cell number counting, cell cycle analysis with propidium iodide staining and fluorescence‑activated cell sorting (FACS) analysis, apoptotic rate with Annexin V staining and FACS analysis, as well as colony formation assay in GC cells. The results revealed that CTDP1 was highly expressed in certain GC cell lines and lentivirus‑mediated siRNA infection was able to effectively silence CTDP1 expression in GC cells. CTDP1 inhibition decreased cell proliferation, arrested the cell cycle at G0/G1 phase and increased cell apoptosis in GC cells. Furthermore, the colony formation ability of GC cells was also suppressed by silencing CTDP1. Taken together these results indicated that CTDP1 has a significant role in the tumor formation ability of GC cells and is a novel and promising therapeutic target for the treatment of GC.

View Figures

View References

1	Torpy JM, Lynm C and Glass RM: JAMA patient page. Stomach cancer. JAMA. 303:17712010. View Article : Google Scholar : PubMed/NCBI
2	Siegel R, Ma J, Zou Z and Jemal A: Cancer statistics, 2014. CA Cancer J Clin. 64:9–29. 2014. View Article : Google Scholar : PubMed/NCBI
3	Jang BG and Kim WH: Molecular pathology of gastric carcinoma. Pathobiology. 78:302–310. 2011. View Article : Google Scholar : PubMed/NCBI
4	Chambers RS and Dahmus ME: Purification and characterization of a phosphatase from HeLa cells which dephosphorylates the C-terminal domain of RNA polymerase II. J Biol Chem. 269:26243–26248. 1994.PubMed/NCBI
5	Maniatis T and Reed R: An extensive network of coupling among gene expression machines. Nature. 416:499–506. 2002. View Article : Google Scholar : PubMed/NCBI
6	Kalaydjieva L: Congenital cataracts-facial dysmorphism-neuropathy. Orphanet J Rare Dis. 1:322006. View Article : Google Scholar : PubMed/NCBI
7	Varon R, Gooding R, Steglich C, Marns L, Tang H, Angelicheva D, Yong KK, Ambrugger P, Reinhold A, Morar B, et al: Partial deficiency of the C-terminal-domain phosphatase of RNA polymerase II is associated with congenital cataracts facial dysmorphism neuropathy syndrome. Nat Genet. 35:185–189. 2003. View Article : Google Scholar : PubMed/NCBI
8	Proudfoot NJ, Furger A and Dye MJ: Integrating mRNA processing with transcription. Cell. 108:501–512. 2002. View Article : Google Scholar : PubMed/NCBI
9	Svetlov V and Nudler E: Basic mechanism of transcription by RNA polymerase II. Biochim Biophys Acta. 1829:20–28. 2013. View Article : Google Scholar : PubMed/NCBI
10	Dahmus ME: Reversible phosphorylation of the C-terminal domain of RNA polymerase II. J Biol Chem. 271:19009–19012. 1996. View Article : Google Scholar : PubMed/NCBI
11	Jasnovidova O and Stefl R: The CTD code of RNA polymerase II: A structural view. Wiley Interdiscip Rev RNA. 4:1–16. 2013. View Article : Google Scholar : PubMed/NCBI
12	Buratowski S: The CTD code. Nat Struct Biol. 10:679–680. 2003. View Article : Google Scholar : PubMed/NCBI
13	Bierhoff H, Dundr M, Michels AA and Grummt I: Phosphorylation by casein kinase 2 facilitates rRNA gene transcription by promoting dissociation of TIF-IA from elongating RNA polymerase I. Mol Cell Biol. 28:4988–4998. 2008. View Article : Google Scholar : PubMed/NCBI
14	Visconti R, Palazzo L, Monica R Della and Grieco D: Fcp1-dependent dephosphorylation is required for M-phase-promoting factor inactivation at mitosis exit. Nat Commun. 3:8942012. View Article : Google Scholar : PubMed/NCBI
15	Hégarat N, Vesely C, Vinod PK, Ocasio C, Peter N, Gannon J, Oliver AW, Novák B and Hochegger H: PP2A/B55 and Fcp1 regulate Greatwall and Ensa dephosphorylation during mitotic exit. PLoS Genet. 10:e10040042014. View Article : Google Scholar : PubMed/NCBI
16	Schauer T, Tombácz I, Ciurciu A, Komonyi O and Boros IM: Misregulated RNA Pol II C-terminal domain phosphorylation results in apoptosis. Cell Mol Life Sci. 66:909–918. 2009. View Article : Google Scholar : PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Carboxy‑terminal domain phosphatase 1 silencing results in the inhibition of tumor formation ability in gastric cancer cells

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Related Articles